Salmonella enteritidis in Eggs from Commercial Chicken Layer Flocks Implicated in Human Outbreaks

Henzler, David J.; Ebel, Eric D.; Sanders, John P.; Kradel, David C.; Mason, John

doi:10.2307/1591834

Cited by 81 publications

(44 citation statements)

References 3 publications

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“…14 However, it should be mentioned that Belgium has many laying hens compared to 15 neighbouring countries (Quinet, 2005). The European survey was based on environmental 16 sampling which is considered to be an accurate and representative indicator for the presence 17 of Salmonella in layer flocks and for the probability that hens would lay contaminated eggs 18 (Henzler et al, 1994;Kinde et al, 2005). The persistence of the pathogen in the intestinal 19 tract is more important when infection occurs in young chicks, since bacterial clearance 20 occurs more efficiently in adults.…”

Section: Discussionmentioning

confidence: 99%

Salmonella in Belgian laying hens: An identification of risk factors

Namata

Meroc

Aerts

et al. 2008

Preventive Veterinary Medicine

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Section: Discussionmentioning

confidence: 99%

Salmonella in Belgian laying hens: An identification of risk factors

Namata

Meroc

Aerts

et al. 2008

Preventive Veterinary Medicine

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“…Sampling the hen house environment, when coupled with suitable cultural techniques, has proved a sensitive and cost-effective method of monitoring salmonella carriage and excretion by layer hens (Kradel & Miller, 1991;Henzler et al ., 1994), and there is good agreement between the level of environmental contamination and the level of internal egg contamination and associated human disease (Altekruse et al ., 1993;Henzler et al ., 1994Henzler et al ., , 1998Schlosser et al ., 1995;Mallinson et al ., 2000). The sensitivity of environmental sampling varies between sample types (Davies & Wray, 1996), and in caged layers samples taken from egg belts, from dust close to birds or cage stacks and from naturally accumulated pooled chicken faeces on droppings belts or scrapers have proved especially useful .…”

Section: Introductionmentioning

confidence: 99%

A longitudinal study of environmental salmonella contamination in caged and free-range layer flocks

et al. 2007

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The environmental contamination by salmonella was examined over a 12-month period in 74 commercial layer flocks from eight farms in the UK, which previously had been identified as being contaminated with salmonella. Samples of faeces, dust, litter, egg belt spillage and wildlife vectors were taken, plus swabs of cages, feeders, drinkers, floors, egg belts and boots. Some sampling was performed in each month of the year. Numerous serovars were detected but Salmonella enterica serotype Enteritidis was the only persistent serotype found among single-age flocks. There was a significant correlation between qualitative environmental samples and semi-quantitative faeces samples. The level of environmental contamination increased significantly over time. There were significant temperature and seasonal effects upon contamination. Wildlife vectors proved to be sensitive samples for the detection of salmonella. The efficacy of cleaning and disinfection upon residual salmonella contamination, and upon subsequent flock contamination, was highly variable between and within premises. The variability between detected prevalences over time and between flocks indicates a need for regular, sensitive monitoring of flocks for salmonella to permit targeting of control measures aimed at eliminating contamination of the layer environment by salmonella. There is substantial scope for improvement of cleaning and disinfection procedures.

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“…Egg 3 In addition, it has been reported that Salmonella present on the egg shell surface enters the egg through stomas or cracks (Cho, 2011;Henzler et al, 1994;Jang et al, 1999). As evident from the test results conducted in the present study, bacteria were isolated from both egg contents and egg shells, identical results were obtained from antibiotic resistance tests, and the isolates showed more than 99% homology.…”

Section: Resultsmentioning

confidence: 99%

First Report on Isolation of Salmonella Enteritidis from Eggs at Grocery Stores in Korea

Kim¹,

Song²,

Lim³

et al. 2013

Korean Journal for Food Science of Animal Resources

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Salmonella Enteritidis is responsible for causing foodborne diseases upon consumption of egg products. While cases of S. Enteritidis isolation from eggs have been reported in other countries, no such cases have previously been reported in Korea. In this study, we report the first isolation and identification of S. Enteritidis from domestically distributed eggs in Korea. Eggs were collected from eight countrywide grocery stores during different seasons between 2011 and 2012. Egg contents and washing solution of egg shells were incubated in buffered peptone water, and the enriched broth was further enriched in tetrathionate broth and Rappaport-Vassiliadis. The secondary enriched broth was streaked on xylose lysine desoxycholate agar. The suspected colonies were confirmed to S. Enteritidis by a biochemical test, serotyping, and PCR test. Genetic relatedness among the isolates was analyzed using Diversilab Salmonella kit. Three strains of S. Enteritidis were isolated from egg contents and egg shells collected from grocery stores of the Eumseong-city in the fall of 2011. All three stains showed resistance to chloramphenicol, streptomycin, nalidixic acid, and ampicillin by the disk diffusion method. In addition, the isolates showed more than 99% DNA homology, indicating that they were presumably identical strains. Therefore, there is a requirement to monitor and control against S. Enteritidis from eggs in Korea.

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Salmonella enteritidis in Eggs from Commercial Chicken Layer Flocks Implicated in Human Outbreaks

Cited by 81 publications

References 3 publications

Salmonella in Belgian laying hens: An identification of risk factors

Salmonella in Belgian laying hens: An identification of risk factors

A longitudinal study of environmental salmonella contamination in caged and free-range layer flocks

First Report on Isolation of Salmonella Enteritidis from Eggs at Grocery Stores in Korea

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