Salmonella Enteritidis in chicken carcasses and giblets in Southern Chile

Ulloa, J; González, Mercedes Hernández; Hernandez-Suarez, Carlos; Villanueva, María Paz; Fernández, Heriberto

doi:10.3855/jidc.623

Cited by 8 publications

(3 citation statements)

References 8 publications

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“…Different Salmonella serotypes were identified from 1-7 days old chicken (S. Enteritidis, S. Typhimurium, S. infantis, S. apeyeme and S. Bargny) and from birds of more than 7 days old (S. Enteritidis, S. Typhimurium, S. Newport and S. Magherafelt). These results are in agreement with other studies [36][37]. These findings support the assumption that there is a difference in the occurrence of some salmonella types at young ages versus older ages [38][39][40].…”

Section: Discussionsupporting

confidence: 93%

Effect of Age and Season on Salmonella Infection in Broiler Chickens with Special Reference to Virulence and Antibiotic Resistance Genes

Roshdy

Mohamed

Elsebaey³

et al. 2020

Zagazig Veterinary Journal

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Salmonella species are causing high morbidity and mortality rates in broiler chickens and other birds particularly young ages resulting in great economic losses. The present study was performed to detect the effect of age and season on isolation rates of Salmonella species from 120 examined broiler chickens with different ages (1-7 days-old and more than 7days-old) in different seasons (summer, winter, autumn and spring). The highest isolation rates of Salmonella species were obtained from broilers of 1-7 daysold in summer (40%) followed by broilers of more than 7 days old in summer (33.3%). The increased isolation rates of Salmonella species was detected from liver of broilers of both ages (1-7 and more than 7 days old). Seventeen Salmonella isolates were obtained in this study from both ages. Serotyping of these Salmonella isolates revealed S. Enteritidis (5 isolates), S. Typhimurium (3 isolates), S. Infantis, S. Bargny, S. Newport, S. Magherafelt (2 isolates) and finally S. Apeyeme in (1 isolate). The most frequent resistance phenotypes were tetracycline (70%-71.4%), from 1-7 days-old and more than 7 days-old chicks and finally ampicillin (60%, 57.1%). From the result obtained by using VITEK2 system, the highest resistance was recorded against Tetracycline in all Salmonella species. All Salmonella isolates showed-associated genes. The invA gene was identified in all Salmonella serovars isolated from chickens of the two age groups, whereas, avrA gene was detected in all the isolates except S. Magherafelt recovered from the age group of more than 7 days-old birds. The tetracycline resistance gene (tetA) was identified in all the examined isolates.

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Section: Discussionsupporting

confidence: 93%

Effect of Age and Season on Salmonella Infection in Broiler Chickens with Special Reference to Virulence and Antibiotic Resistance Genes

Roshdy

Mohamed

Elsebaey³

et al. 2020

Zagazig Veterinary Journal

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“…Salmonellosis, mainly caused by Salmonella, is one of the most frequent infectious foodborne diseases around the world. Salmonella enterica serovar Enteritidis (SE) is one of the pathogenic bacteria, which causes signi cant economic losses on poultry production and puts severe threat on human health [1][2][3] through contaminated poultry and egg [4][5][6]{Hohmann, 2001 #37;L, 2009 #41;Currie, 2005 #43}. It is estimated that Salmonella enteritidis causes 1.3 million cases of gastroenteritis, and more than 350 died each year in the United States [7].…”

Section: Introductionmentioning

confidence: 99%

Chicken cecal DNA methylome alteration in the response to Salmonella enterica serovar Enteritidis inoculation

Wang

Liu

et al. 2020

Preprint

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Background: Salmonella enterica serovar Enteritidis (SE) is one of the pathogenic bacteria, which affects poultry production and poses a severe threat to public health. Chicken meat and eggs are the main sources of human salmonellosis. DNA methylation is involved in regulatory processes including gene expression, chromatin structure and genomic imprinting. To understand the methylation regulation in the response to SE inoculation in chicken, the genome-wide DNA methylation profile following SE inoculation was analyzed through whole-genome bisulfite sequencing in the current study. Results: There were 185,362,463 clean reads and 126,098,724 unique reads in the control group, and 180,530,750 clean Reads and 126,782,896 unique reads in the inoculated group. The methylation density in the gene body was higher than that in the upstream and downstream regions of the gene. There were 8,946 differentially methylated genes (3,639 hypo-methylated genes, 5,307 hyper-methylated genes) obtained between inoculated and control groups. Methylated genes were mainly enriched in immune-related Gene Ontology (GO) terms and metabolic process terms. Cytokine-cytokine receptor interaction, TGF-beta signaling pathway, FoxO signaling pathway, Wnt signaling pathway and several metabolism-related pathways were significantly enriched. The density of differentially methylated cytosines in miRNAs was the highest. HOX genes were widely methylated. Conclusions: The genome-wide DNA methylation profile in the response to SE inoculation in chicken was analyzed. SE inoculation promoted the DNA methylation in the chicken cecum and caused methylation alteration in immune- and metabolic- related genes. Wnt signal pathway, miRNAs and HOX gene family may play crucial roles in the methylation regulation of SE inoculation in chicken. The findings herein will deepen the understanding of epigenetic regulation in the response to SE inoculation in chicken.

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“…Salmonellosis, mainly caused by Salmonella, is one of the most frequently infectious foodborne diseases around the world. Salmonella enterica serovar Enteritidis (SE) is one of pathogenic bacteria, which causes signi cant economic losses on poultry production and put severe threat on human health [1][2][3] through poultry and egg [4][5][6]. It is estimated that Salmonella enteritidis causes 1.3 million cases of gastroenteritis and more than 350 died each year in the United States [7].…”

Section: Introductionmentioning

confidence: 99%

Cecum methylome response to Salmonella enterica serovar Enteritidis infection in chicken through whole genome bisulfite sequencing

Wang¹,

Liu²,

Li³

et al. 2020

Preprint

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Background: Salmonella enterica serovar Enteritidis (SE) is one of the pathogenic bacteria, which affects poultry production and poses severe threat to public health. Chicken meat and egg are the main source of SE. DNA methylation, an important epigenetic modification, involves in regulatory processes including gene expression, chromatin structure and genomic imprinting. To understand the methylation regulation in response to SE inoculation in chicken, the genome-wide DNA methylation profile following SE inoculation was analyzed through whole genome bisulfite sequencing in the current study. Results: There were 185,362,463 clean reads and 126,098,724 unique reads in the control group, and 180,530,750 clean Reads, 126,782,896 unique reads in the inoculated group. We found that the methylation density in gene body was higher than that in the upstream and downstream regions of gene. There were 8,946 differentially methylated genes (3,639 hypo-methylated genes, 5,307 hyper-methylated genes) obtained between inoculated and control groups. Methylated genes were mainly enriched in immune-related Gene Ontology (GO) terms and metabolic process terms. Cytokine-cytokine receptor interaction, TGF-beta signaling pathway, FoxO signaling pathway, Wnt signaling pathway and several metabolism-related pathways were significantly enriched. The density of differentially methylated cytosines in miRNAs was the highest. HOX genes were widely methylated and mainly distributed in Chr2 and 7. Conclusions: We firstly analyzed the genome-wide DNA methylation in the response to SE inoculation in chicken. SE inoculation promoted the DNA methylation in chicken cecum and caused methylation alteration in immune- and metabolic- related genes. Wnt signal pathway, miRNAs and HOX gene family may play a crucial role in the methylation regulation of SE infection in chicken. The findings herein will deepen the understanding of epigenetic regulation in the response to SE inoculation in chicken.

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Salmonella Enteritidis in chicken carcasses and giblets in Southern Chile

Cited by 8 publications

References 8 publications

Effect of Age and Season on Salmonella Infection in Broiler Chickens with Special Reference to Virulence and Antibiotic Resistance Genes

Effect of Age and Season on Salmonella Infection in Broiler Chickens with Special Reference to Virulence and Antibiotic Resistance Genes

Chicken cecal DNA methylome alteration in the response to Salmonella enterica serovar Enteritidis inoculation

Cecum methylome response to Salmonella enterica serovar Enteritidis infection in chicken through whole genome bisulfite sequencing

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