Salmonella enteritidis ghost vaccine induces effective protection against lethal challenge in specific-pathogen-free chicks

Peng, Wei; Si, Wei; Lu, Yin; Liu, Huifang; Yu, Shenye; Liu, Siguo; Wang, Chunlai; Chang, Yuehong; Zhang, Zhuo; Hu, Shouping; Du, Yiqi

doi:10.1016/j.imbio.2010.10.001

Cited by 46 publications

(23 citation statements)

References 48 publications

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“…A mutant lysis gene E with a single-base mutation at the 5=-terminal start codon ATG (A/C) and a truncated 201-base-pair (bp) lysis gene was inserted into plasmid pBV220, which harbored a thermosensitive regulator system, p R /p L -cI857, from a lambda bacteriophage (9). The lysis cassette gene p R /p L -cI857-mE was amplified by PCR and cloned into plasmid pMD-18T (TaKaRa) to create the bacteriolytic plasmid pMD-mE.…”

Section: Methodsmentioning

confidence: 99%

“…The rHps-mE clone was inoculated into supplemented TSB (with 100 g/ml ampicillin and 10 g/ml NAD), and the culture was incubated at 28°C to an OD 600 of 0.4 to 0.5. The temperature was subsequently raised to 42°C to induce mutant lysis protein mE-mediated lysis (9)(10)(11). The OD 600 was measured every 30 min until no further decrease in OD 600 was After the completion of lysis, the BGs were harvested by centrifugation (4,000 ϫ g for 10 min at 4°C), washed with phosphate-buffered saline (PBS) (pH 7.2), resuspended in sterile distilled water, lyophilized, and stored at Ϫ20°C.…”

Section: Methodsmentioning

confidence: 99%

“…In recent years, a genetically inactivated vaccine-the ghost vaccine-has emerged as an alternative to traditional inactivated bacterin, which lacks cross-protection and fails to prevent subclinical or chronic infection and pathogen colonization (7)(8)(9).…”

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confidence: 99%

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Protection of Piglets by a Haemophilus parasuis Ghost Vaccine against Homologous Challenge

Xie

et al. 2013

Clin Vaccine Immunol

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bCommercial bacterins for Glässer's disease are widely used for the prevention of this disease caused by Haemophilus parasuis; however, the protective efficacy varies depending on the strain and serovar. Bacterial ghosts (BGs) are empty bacterial envelopes that, unlike classic bacterins, suffer no denaturing steps during their production. These properties may lead to superior protection. In this study, a BG vaccine generated from the Haemophilus parasuis serovar 5 reference strain Nagasaki was prepared and used to inoculate piglets. The efficacy of the BG vaccine was evaluated by clinical, bacteriological, serological, and postmortem examinations. Inactivated bacterin (IB) and a placebo control (PC) were compared with the BG vaccine in this study. The results showed that the piglets inoculated with the BG vaccine developed higher antibody activity and higher gamma interferon and interleukin 4 levels than those vaccinated with IB or those in the PC group after primary and secondary exposure to the antigens and challenge. CD4؉ T lymphocyte levels were observed to increase following secondary immunization more in the BG-vaccinated group than in the IB (P < 0.05) and PC (P < 0.05) groups. CD8؉ T lymphocyte levels increased dramatically in all three groups after challenge, and the differences between groups were all significant (P < 0.05). There were fewer tissue lesions and lower bacterial loads in the tissue homogenates in the BG group after challenge. The results suggest that higher CD4 ؉ T lymphocyte levels and both CD4؉ major histocompatibility complex class II-restricted Th1-type and Th2-type immune responses in the BG group are relevant for protection. Haemophilus parasuis is a Gram-negative, nonhemolytic, NAD-dependent bacterium belonging to the Pasteurellaceae family. H. parasuis is a commensal organism of the upper respiratory tract of conventional pigs that causes Glässer's disease, which is characterized by fibrinous polyserositis, polyarthritis, and meningitis (1-3). This disease causes significant losses in the swine industry worldwide, due to its high mortality and morbidity rates (1, 3). Vaccination generally is considered to be the most effective means to control Glässer's disease; however, attempts to develop effective vaccines against H. parasuis have been hampered by a lack of overall knowledge regarding the virulence factors and protective antigens of this bacterium (1). Inactivated bacterin for H. parasuis is a traditional vaccine used around the world that can elicit efficient protection against homologous challenges; however, due to the serovar diversity of H. parasuis, inactivated bacterin is limited in cross-protection (2-4). In a previous study, subunit vaccines comprising recombinant transferrin-binding protein B (TbpB) or outer membrane protein (OMP) formulations enriched with TbpB conferred only partial protection (5). The OMPs PalA, Omp2, D15, and HPS-06257 induced protective responses against H. parasuis (SH0165) in pigs (3), and the OMPs SmpA, YgiW, and FOG have been shown to be prote...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Protection of Piglets by a Haemophilus parasuis Ghost Vaccine against Homologous Challenge

Xie

et al. 2013

Clin Vaccine Immunol

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“…Since bacterial ghosts maintain the functional and antigenic envelope structures of their native live counterparts, these ghosts are capable of inducing strong humoral and cellmediated immune responses. For example, Salmonella ghosts have been shown to induce protective immune responses in chickens (9)(10)(11).…”

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confidence: 99%

Salmonella enterica Serovar Enteritidis Ghosts Carrying the Escherichia coli Heat-Labile Enterotoxin B Subunit Are Capable of Inducing Enhanced Protective Immune Responses

Jawale

Lee

2014

Clin Vaccine Immunol

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The Escherichia coli heat-labile enterotoxin B subunit (LTB) is a potent vaccine adjuvant. Salmonella enterica serovar Enteritidis ghosts carrying LTB (S. Enteritidis-LTB ghosts) were genetically constructed using a novel plasmid, pJHL187-LTB, designed for the coexpression of the LTB and E lysis proteins. S. Enteritidis-LTB ghosts were characterized using scanning electron microscopy to visualize their transmembrane tunnel structures. The expression of LTB in S. Enteritidis-LTB ghost preparations was confirmed by immunoblot and enzyme-linked immunosorbent assays. The parenteral adjuvant activity of LTB was demonstrated by immunizing chickens with either S. Enteritidis-LTB ghosts or S. Enteritidis ghosts. Chickens were intramuscularly primed at 5 weeks of age and subsequently boosted at 8 weeks of age. In total, 60 chickens were equally divided into three groups

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“…Vaccination has been used as a preventive measure and also for controlling Salmonella Enteritidis outbreaks worldwide (He et al, 2010;Peng et al, 2011). Up to day, few data are present in the literature about the quantitative analysis of live Salmonella Enteritidis live vaccine in chicken at different times following vaccination.The mechanism for the rapid induction of immunity of attenuated Salmonella Enteritidis vaccine remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Replication Kinetics of <I>Salmonella</I> Enteritidis Live Vaccine in the Immune Organs of Chicken after Subcutaneous Immunization

Deng²

2012

J. Poult. Sci.

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We used a real-time PCR for Salmonella Enteritidis to detect the genomic DNA of Salmonella Enteritidis live vaccine in the immune organs, including the bursa of Fabricius, thymus, spleen, and Harderian gland, from chicken after subcutaneously vaccinated at different time points. Significant numbers of Salmonella Enteritidis genomes in the immune organs were first detected at 12 hour (post-vaccination) p.v., and subsequently rose to peak levels during 48 h to 72 h p.v. The rapid early increase of vaccine levels in all samples examined followed by a steady decline from 84 h to 15 days p.v. The real-time PCR analysis of a variety of tissues is significant for further investigation of the mechanism of vaccinal protection, and the optimization of vaccination regimes.

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Salmonella enteritidis ghost vaccine induces effective protection against lethal challenge in specific-pathogen-free chicks

Cited by 46 publications

References 48 publications

Protection of Piglets by a Haemophilus parasuis Ghost Vaccine against Homologous Challenge

Protection of Piglets by a Haemophilus parasuis Ghost Vaccine against Homologous Challenge

Salmonella enterica Serovar Enteritidis Ghosts Carrying the Escherichia coli Heat-Labile Enterotoxin B Subunit Are Capable of Inducing Enhanced Protective Immune Responses

Replication Kinetics of <I>Salmonella</I> Enteritidis Live Vaccine in the Immune Organs of Chicken after Subcutaneous Immunization

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