Salmonella enterica colonization and fitness in pre-harvest cantaloupe production

Burris, Kellie P.; Simmons, Otto D.; Webb, Hannah M.; Moore, Robin Grant; Jaykus, Lee‐Ann; Zheng, Jie; Reed, Elizabeth; Ferreira, Christina M.; Brown, Eric W.; Bell, Rebecca

doi:10.1016/j.fm.2020.103612

Cited by 11 publications

(4 citation statements)

References 34 publications

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“…Similarly, between 1973 and 2011, researchers identified 19 U.S. outbreaks of foodborne disease involving cantaloupes, wherein Salmonella was the most commonly identified etiologic agent, causing 1,012 recorded disease cases and 38 fatalities, including one fetal death (Walsh et al, 2014). Burris et al (2021) recently discussed Salmonella capabilities to contaminate melon fruit through blossom contamination as well as internalization into cantaloupe flesh as drivers of melon transmission of the pathogen to consumers.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial-Loaded Polymeric Micelles Inhibit Enteric Bacterial Pathogens on Spinach Leaf Surfaces During Multiple Simulated Pathogen Contamination Events

Yegin

Perez-Lewis

Liu

et al. 2021

Front. Sustain. Food Syst.

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Protecting fresh-packed produce microbiological safety against pre- and post-harvest microbial pathogen contamination requires innovative antimicrobial strategies. Although largely ignored in the scientific literature, there exists the potential for gross failure in food safety protection of fresh fruits and vegetables leading to opportunity for multiple produce contamination events to occur during production and post-harvest handling of food crops. The primary objective of this research was to determine the efficacy of plant-derived antimicrobial-loaded nanoparticles to reduce Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium on spinach leaf surfaces whilst simulating multiple pathogen contamination events (pre-harvest and post-harvest). Spinach samples were inoculated with a blend of E. coli O157:H7 and S. Typhimurium, each diluted to ~8.0 log10 CFU/mL. The inoculated samples were then submerged in solutions containing nanoparticles loaded with geraniol (GPN; 0.5 wt.% geraniol), unencapsulated geraniol (UG; 0.5 wt.%), or 200 ppm chlorine (HOCl; pH 7.0), with untreated samples serving for controls. Following antimicrobial treatment application, samples were collected for surviving pathogen enumeration or were placed under refrigeration (5°C) for up to 10 days, with periodic enumeration of pathogen loads. After 3 days of refrigerated storage, all samples were removed, aseptically opened and subjected to a second inoculation with both pathogens. Treatment of spinach surfaces with encapsulated geraniol reduced both pathogens to non-detectable numbers within 7 days of refrigerated storage, even with a second contamination event occurring 3 days after experiment initiation. Similar results were observed with the UG treatment, except that upon recontamination at day 3, a higher pathogen load was detected on UG-treated spinach vs. GPN-treated spinach. These data fill a research gap by providing a novel tool to reduce enteric bacterial pathogens on spinach surfaces despite multiple contamination events, a potential food safety risk for minimally processed edible produce.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial-Loaded Polymeric Micelles Inhibit Enteric Bacterial Pathogens on Spinach Leaf Surfaces During Multiple Simulated Pathogen Contamination Events

Yegin

Perez-Lewis

Liu

et al. 2021

Front. Sustain. Food Syst.

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“…Furthermore, contaminated water used for irrigation or other foliar applications, where water comes in contact with the edible portion of the plants, is of great concern. Studies involving flowering fruit and vegetable crops have demonstrated that exposure of the blossom to Salmonella often leads to externally and internally contaminated fruit [58][59][60]. For leafy vegetables, others have shown that Salmonella is able to enter the apoplast of the plant through the stomata [61,62].…”

Section: Salmonellamentioning

confidence: 99%

“…For leafy vegetables, others have shown that Salmonella is able to enter the apoplast of the plant through the stomata [61,62]. Moreover, Salmonella has been shown to invade the root system of several plant varieties shortly after transplant, while the plant is still in transplant shock [58][59][60]. In all these instances, once Salmonella has internalized into the plant, post-harvest sanitation steps will not be able to reach or eliminate the pathogen.…”

Section: Salmonellamentioning

confidence: 99%

The Persistence of Bacterial Pathogens in Surface Water and Its Impact on Global Food Safety

et al. 2021

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Water is vital to agriculture. It is essential that the water used for the production of fresh produce commodities be safe. Microbial pathogens are able to survive for extended periods of time in water. It is critical to understand their biology and ecology in this ecosystem in order to develop better mitigation strategies for farmers who grow these food crops. In this review the prevalence, persistence and ecology of four major foodborne pathogens, Shiga toxin-producing Escherichia coli (STEC), Salmonella, Campylobacter and closely related Arcobacter, and Listeria monocytogenes, in water are discussed. These pathogens have been linked to fresh produce outbreaks, some with devastating consequences, where, in a few cases, the contamination event has been traced to water used for crop production or post-harvest activities. In addition, antimicrobial resistance, methods improvements, including the role of genomics in aiding in the understanding of these pathogens, are discussed. Finally, global initiatives to improve our knowledge base of these pathogens around the world are touched upon.

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“…Internalization of pathogens depends upon different factors such as plant species, plant age, microbial ecology, contamination routes and levels of contamination (Deering et al, 2012;Ge et al, 2014). Many internalization studies have focused on vegetables (Erickson, 2012;Hirneisen et al, 2012) and fruits such as tomatoes, melons and cucumbers (Bartz et al, 2015;Burris et al, , 2021. In 2014, the Panel on Biological Hazards of EFSA published a Scientific Opinion highlighting the paucity of information on the potential of Salmonella or Norovirus to be internalized within berries or plants (EFSA, 2014).…”

Section: )mentioning

confidence: 99%

Internalization capacity of Salmonella enterica sv Thompson in strawberry plants via root

et al. 2021

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Salmonella enterica colonization and fitness in pre-harvest cantaloupe production

Cited by 11 publications

References 34 publications

Antimicrobial-Loaded Polymeric Micelles Inhibit Enteric Bacterial Pathogens on Spinach Leaf Surfaces During Multiple Simulated Pathogen Contamination Events

Antimicrobial-Loaded Polymeric Micelles Inhibit Enteric Bacterial Pathogens on Spinach Leaf Surfaces During Multiple Simulated Pathogen Contamination Events

The Persistence of Bacterial Pathogens in Surface Water and Its Impact on Global Food Safety

Internalization capacity of Salmonella enterica sv Thompson in strawberry plants via root

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