Salmonella carrier-state in hens: study of host resistance by a gene expression approach

Sadeyen, Jean-Rémy; Trotereau, Jérôme; Protais, J.; Beaumont, Catherine; Sellier, Nadine; Salvat, Gilles; Velge, Philippe; Lalmanach, Anne-Christine

doi:10.1016/j.micinf.2005.12.014

Cited by 60 publications

(53 citation statements)

References 42 publications

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“…Those authors observed similar results when working with 30-week-old chickens challenged with the same bacterial strain (Sadeyen et al, 2006). Another study testing layer hens challenged with S. Enteritidis Y-24 (phage type 4) showed that the highest colonization in the caecum was achieved on days 3 and 4 post-challenge (Piao et al, 2007).…”

Section: Discussionmentioning

confidence: 57%

“…compared with those of similar studies, which have tended to range from 10 4 to 10 8 c.f.u. (Berndt et al, 2007;Bohez et al, 2006;Sadeyen et al, 2004Sadeyen et al, , 2006. Preliminary experiments done by our group (data not shown) involving the challenge of 21-day-old broiler chickens and the challenge of 1-day-old SPF chickens with 10 6 , 10 8 and 10 10 c.f.u.…”

Section: Discussionmentioning

confidence: 80%

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Salmonella enterica subspecies enterica serovar Enteritidis Salmonella pathogenicity island 2 type III secretion system: role in intestinal colonization of chickens and systemic spread

et al. 2010

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Salmonella enterica subspecies enterica serovar Enteritidis (S. Enteritidis) has been identified as a significant cause of salmonellosis in humans. Salmonella pathogenicity islands 1 and 2 (SPI-1 and SPI-2) each encode a specialized type III secretion system (T3SS) that enables Salmonella to manipulate host cells at various stages of the invasion/infection process. For the purposes of our studies we used a chicken isolate of S. Enteritidis (Sal18). In one study, we orally co-challenged 35-day-old specific pathogen-free (SPF) chickens with two bacterial strains per group. The control group received two versions of the wild-type strain Sal18: Sal18 attTn7 : : tet and Sal18 attTn7 : : cat, while the other two groups received the wild-type strain (Sal18 attTn7 : : tet) and one of two mutant strains. From this study, we concluded that S. Enteritidis strains deficient in the SPI-1 and SPI-2 systems were outcompeted by the wild-type strain. In a second study, groups of SPF chickens were challenged at 1 week of age with four different strains: the wild-type strain, and three other strains lacking either one or both of the SPI-1 and SPI-2 regions. On days 1 and 2 post-challenge, we observed a reduced systemic spread of the SPI-2 mutants, but by day 3, the systemic distribution levels of the mutants matched that of the wild-type strain. Based on these two studies, we conclude that the S. Enteritidis SPI-2 T3SS facilitates invasion and systemic spread in chickens, although alternative mechanisms for these processes appear to exist.

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Section: Discussionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 80%

Salmonella enterica subspecies enterica serovar Enteritidis Salmonella pathogenicity island 2 type III secretion system: role in intestinal colonization of chickens and systemic spread

et al. 2010

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“…Sadeyen et al [14] compared adult hens issued from poultry lines with different rates of Salmonella carrier-state; they observed differences in expression levels of several genes involved in primary immune responses while Proux et al [11] showed the existence of differences in immune humoral response (appreciated by antibody response) between two lines (among which the L2 line). Protais et al [10] obtained similar results on different inbred lines among which those studied by Sadeyen et al [14]; moreover these authors showed that these differences were associated with different vaccine efficiencies, which is in favor of a lower rate or return to the susceptible state in the lines showing higher antibody response. Our parameter estimates make it possible to compare kinetics of Salmonella propagation in the two lines: differences were more marked for the percentage of contamination at the peak than for the duration of contamination.…”

Section: Discussionmentioning

confidence: 99%

Effect of genetic resistance of the hen toSalmonellacarrier-state on incidence of bacterial contamination: synergy with vaccination

Prevost

Magal

Protais³

et al. 2008

Vet. Res.

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-Salmonella is one of the major sources of toxi-infections in humans. The association between egg consumption and Salmonella outbreaks is a serious economic and public health problem. To control the incidence of Salmonella in poultry flocks, many prophylactic means have been developed but none allows a total reduction of the risk. In a previous study, we derived mathematical models for Salmonella transmission and used them to appreciate the most important factors of variation of egg contamination rate and thus of risk of human contamination. Thanks to recent data of a selection experiment for increased or decreased rate of carrier-state (also called divergent selection), we showed that mixing, in an equal proportion, animals issued from a line selected for a lower (denoted Sal-) or higher propensity to carry Salmonella (denoted Sal+) results in a reduction by half of the maximal percentage of contaminated animals but does not accelerate the extinction of the disease. Vaccination and selection should be synergic, since a former contamination reduces the maximal prevalence by 45 and 71%, respectively, in Sal+ or Sal-flocks respectively. These results show the interest in the introduction, even at a rather moderate percentage, of animals selected for a reduced rate of Salmonella carrier-state within commercial flocks. This could be achieved by using one or more selected lines in commercial crosses. These results must be confirmed experimentally while the mathematical model could be extended with minor modifications to other animal species or pathogenic species. Salmonella / mathematical model / genetic / resistance / vaccination

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“…They may not be extrapolated to hens without experimental validation. Indeed, differences in expression of gallinacins observed by Sadeyen et al, (2004) and Sadeyen et al (2006) were found to be associated, in young chicken, with increased susceptibility but, in adults, with resistance. Similar variations with age should also be the case of a large proportion of genes or genome regions found to be involved in resistance (for a review, see Calenge et al, 2010).…”

Section: Response To Selectionmentioning

confidence: 95%

“…They observed differences between outbred poultry lines. Later on, Sadeyen et al, (2006) identified differences between inbred lines using the same protocol while Lindell et al, (1994) used a slightly different protocol.…”

Section: Development Of Protocols Of Measures and Identification Of Gmentioning

confidence: 99%

Use of Integrated Studies to Elucidate Potential Benefits from Genetic Resistance to Salmonella Carrier State in Fowl

Beaumont¹,

Tran²,

Anne-France³

et al. 2012

Salmonella - Distribution, Adaptation, Control Measures and Molecular Technologies

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Salmonella carrier-state in hens: study of host resistance by a gene expression approach

Cited by 60 publications

References 42 publications

Salmonella enterica subspecies enterica serovar Enteritidis Salmonella pathogenicity island 2 type III secretion system: role in intestinal colonization of chickens and systemic spread

Salmonella enterica subspecies enterica serovar Enteritidis Salmonella pathogenicity island 2 type III secretion system: role in intestinal colonization of chickens and systemic spread

Effect of genetic resistance of the hen toSalmonellacarrier-state on incidence of bacterial contamination: synergy with vaccination

Use of Integrated Studies to Elucidate Potential Benefits from Genetic Resistance to Salmonella Carrier State in Fowl

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