Salmonella carrier state in chicken: comparison of expression of immune response genes between susceptible and resistant animals

Sadeyen, Jean-Rémy; Trotereau, Jérôme; Velge, Philippe; Marly, J; Beaumont, Catherine; Barrow, Paul; Bumstead, Nat; Lalmanach, Anne-Christine

doi:10.1016/j.micinf.2004.07.005

Cited by 117 publications

(84 citation statements)

References 48 publications

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“…The overall annual incidence of foodborne salmonellosis in India is nearly 6 per 1,000 inhabitants [20]. The most commonly implicated serotypes for disease outbreaks are Salmonella enterica serotype Typhimurium and Salmonella enterica serotype Enteritidis [21]. Non-typhoid salmonellosis originating from foods of animal origin is an emerging infection in India and is noted to be an increasing trend [22].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of microorganisms of hygienic interest in an organized abattoir in Mumbai, India

Bhandare

Paturkar

Waskar

et al. 2010

J Infect Dev Ctries

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Background: The magnitude of food-borne illnesses in India is unknown because of lack of surveillance networks. Monitoring the prevalence of food-borne pathogens and indicators of contamination in primary production at abattoirs is imperative for creating a data bank and for effective control of such pathogens before they enter the food chain. Methodology: Microorganisms of hygienic interest were screened for their prevalence at Deonar Abattoir, Mumbai. Swab samples from 96 sheep/goat carcass sites were collected and analyzed for Staphylococcus spp., Bacillaceae, Clostridiaceae and Enterobacteriaceae. Results: Average Staphylococcus aureus and Staphylococcus epidermidis counts were 3.15 ± 0.18 and 3.46 ± 0.17 log 10 CFU/cm 2 , respectively. Bacillus cereus, Bacillus subtilis and Clostridium spp. counts were 3.10 ± 0.08, 3.41 ± 0.19 and 0.76 ± 0.06 log 10 CFU/cm 2 , respectively. The Escherichia coli count was 3.54 ± 0.06 and the Klebsiella aerogenes count was 3.22 ± 0.22 log 10 CFU/cm 2 . Counts for Proteus vulgaris and Proteus mirabilis were 3.44 ± 0.14 log 10 CFU/cm 2 and 3.71 ± 0.12 log10 CFU/cm 2 , respectively. S. epidermidis had the highest percentage prevalence at (41.6%), followed by K. aerogenes (31.9%), B. subtilis (28.2%) and P. vulgaris (23.6%). Salmonella spp. were not isolated.Conclusions: The data demonstrate high prevalence and diversity of micro flora on carcasses in the primary Indian production facility, which might be attributed to either human handling or improper dressing especially during evisceration process. Appropriate training for personal and production hygiene is essential for workers in Indian meat production facilities.

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Section: Discussionmentioning

confidence: 99%

Prevalence of microorganisms of hygienic interest in an organized abattoir in Mumbai, India

Bhandare

Paturkar

Waskar

et al. 2010

J Infect Dev Ctries

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“…An earlier study, testing colonization levels of 1-week-old chickens challenged with S. Enteritidis strain 1009 on days 7, 14, 28, 35 and 42 post-challenge, showed maximal colonization levels of the spleen and caecum on day 7 (Sadeyen et al, 2004). Those authors observed similar results when working with 30-week-old chickens challenged with the same bacterial strain (Sadeyen et al, 2006).…”

Section: Discussionmentioning

confidence: 57%

“…compared with those of similar studies, which have tended to range from 10 4 to 10 8 c.f.u. (Berndt et al, 2007;Bohez et al, 2006;Sadeyen et al, 2004Sadeyen et al, , 2006. Preliminary experiments done by our group (data not shown) involving the challenge of 21-day-old broiler chickens and the challenge of 1-day-old SPF chickens with 10 6 , 10 8 and 10 10 c.f.u.…”

Section: Discussionmentioning

confidence: 76%

Salmonella enterica subspecies enterica serovar Enteritidis Salmonella pathogenicity island 2 type III secretion system: role in intestinal colonization of chickens and systemic spread

et al. 2010

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Salmonella enterica subspecies enterica serovar Enteritidis (S. Enteritidis) has been identified as a significant cause of salmonellosis in humans. Salmonella pathogenicity islands 1 and 2 (SPI-1 and SPI-2) each encode a specialized type III secretion system (T3SS) that enables Salmonella to manipulate host cells at various stages of the invasion/infection process. For the purposes of our studies we used a chicken isolate of S. Enteritidis (Sal18). In one study, we orally co-challenged 35-day-old specific pathogen-free (SPF) chickens with two bacterial strains per group. The control group received two versions of the wild-type strain Sal18: Sal18 attTn7 : : tet and Sal18 attTn7 : : cat, while the other two groups received the wild-type strain (Sal18 attTn7 : : tet) and one of two mutant strains. From this study, we concluded that S. Enteritidis strains deficient in the SPI-1 and SPI-2 systems were outcompeted by the wild-type strain. In a second study, groups of SPF chickens were challenged at 1 week of age with four different strains: the wild-type strain, and three other strains lacking either one or both of the SPI-1 and SPI-2 regions. On days 1 and 2 post-challenge, we observed a reduced systemic spread of the SPI-2 mutants, but by day 3, the systemic distribution levels of the mutants matched that of the wild-type strain. Based on these two studies, we conclude that the S. Enteritidis SPI-2 T3SS facilitates invasion and systemic spread in chickens, although alternative mechanisms for these processes appear to exist.

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“…They may not be extrapolated to hens without experimental validation. Indeed, differences in expression of gallinacins observed by Sadeyen et al, (2004) and Sadeyen et al (2006) were found to be associated, in young chicken, with increased susceptibility but, in adults, with resistance. Similar variations with age should also be the case of a large proportion of genes or genome regions found to be involved in resistance (for a review, see Calenge et al, 2010).…”

Section: Response To Selectionmentioning

confidence: 96%

“…Studies compared levels of expression before and after infection, on animals from the same genotypes, or between animals issued from resistant and susceptible poultry lines (for a review, see Calenge et al, 2010). Focussing on the expression of candidate immune genes allows a better understanding of their role (as in Sadeyen et al, 2004;Sadeyen et al, 2006;Swaggerty et al, 2006) in relation with the type of cells as shown by Chausse et al, (2011), when comparing results obtained on the whole caeca by Sadeyen et al (2004) …”

Section: Towards Identification Of Genes or Genome Regions Involved Imentioning

confidence: 99%

Use of Integrated Studies to Elucidate Potential Benefits from Genetic Resistance to Salmonella Carrier State in Fowl

Beaumont¹,

Tran²,

Anne-France³

et al. 2012

Salmonella - Distribution, Adaptation, Control Measures and Molecular Technologies

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Salmonella carrier state in chicken: comparison of expression of immune response genes between susceptible and resistant animals

Cited by 117 publications

References 48 publications

Prevalence of microorganisms of hygienic interest in an organized abattoir in Mumbai, India

Prevalence of microorganisms of hygienic interest in an organized abattoir in Mumbai, India

Salmonella enterica subspecies enterica serovar Enteritidis Salmonella pathogenicity island 2 type III secretion system: role in intestinal colonization of chickens and systemic spread

Use of Integrated Studies to Elucidate Potential Benefits from Genetic Resistance to Salmonella Carrier State in Fowl

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