Salmonella as an Innovative Therapeutic Antitumor Agent

Chang, Wen‐Wei; Lee, Che‐Hsin

doi:10.3390/ijms150814546

Cited by 52 publications

(47 citation statements)

References 35 publications

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“…There are many advantages of using Salmonella for cancer treatment, including tumor-targeting, immunostimulation and low cost [10, 11]. Salmonella stimulates host immunity and reduces tumor growth [12, 13].…”

Section: Introductionmentioning

confidence: 99%

Salmonella inhibits tumor angiogenesis by downregulation of vascular endothelial growth factor

Chang

Lin

et al. 2016

Oncotarget

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Salmonella is a Gram-negative, facultative anaerobe that is a common cause of host intestinal infections. Salmonella grows under aerobic and anaerobic conditions, and it has been proven capable of inhibiting tumor growth. However, the molecular mechanism by which Salmonella inhibits tumor growth is still unclear. Angiogenesis plays an important role in the development and progression of tumors. We investigated the antitumor effect of Salmonella in a syngeneic murine tumor model. Hypoxia-inducible factor-1 (HIF-1)α plays a significant role in tumor angiogenesis. We examined the molecular mechanism by which Salmonella regulated vascular endothelial growth factor (VEGF), which is an important angiogenic factor. The expression of VEGF in tumor cells was decreased by treatment with Salmonella. The conditioned medium from Salmonella-treated cells inhibited the proliferation of endothelial cells. Salmonella inhibited the expression of HIF-1α as well as downregulated its upstream signal mediator protein kinase B (AKT). Salmonella significantly inhibited tumor growth in vivo, and immunohistochemical studies of the tumors revealed decreased intratumoral microvessel density. These results suggest that Salmonella therapy, which exerts anti-angiogenic activities, represents a promising strategy for the treatment of tumors.

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Section: Introductionmentioning

confidence: 99%

Salmonella inhibits tumor angiogenesis by downregulation of vascular endothelial growth factor

Chang

Lin

et al. 2016

Oncotarget

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“…In another study, when Salmonella was treated synergistically with IFN-γ, it reduced the cytokine production through increasing PD-L1 expression in intestinal epithelial cells (IECs) [26]. Nevertheless, in the tumor immunosuppressive microenvironment, the mechanism of Salmonella in inhibiting tumor growth involves not only recruiting peripheral natural killer and T cells but also cross-presentation of tumor antigens [27]. On top of these, the present study also showed that immune checkpoint molecules played an important role as a target in the multifaceted interplay between Salmonella and tumor cells.…”

Section: Discussionmentioning

confidence: 99%

Salmonella Breaks Tumor Immune Tolerance by Downregulating Tumor Programmed Death-Ligand 1 Expression

Chen

Pangilinan

Lee

2019

Cancers

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Immunotherapy is becoming a popular treatment modality in combat against cancer, one of the world’s leading health problems. While tumor cells influence host immunity via expressing immune inhibitory signaling proteins, some bacteria possess immunomodulatory activities that counter the symptoms of tumors. The accumulation of Salmonella in tumor sites influences tumor protein expression, resulting in T cell infiltration. However, the molecular mechanism by which Salmonella activates T cells remains elusive. Many tumors have been reported to have high expressions of programmed death-ligand 1 (PD-L1), which is an important immune checkpoint molecule involved in tumor immune escape. In this study, Salmonella reduced the expression of PD-L1 in tumor cells. The expression levels of phospho-protein kinase B (P-AKT), phospho-mammalian targets of rapamycin (P-mTOR), and the phospho-p70 ribosomal s6 kinase (P-p70s6K) pathway were revealed to be involved in the Salmonella-mediated downregulation of PD-L1. In a tumor-T cell coculture system, Salmonella increased T cell number and reduced T cell apoptosis. Systemic administration of Salmonella reduced the expressions of PD-L-1 in tumor-bearing mice. In addition, tumor growth was significantly inhibited along with an enhanced T cell infiltration following Salmonella treatment. These findings suggest that Salmonella acts upon the immune checkpoint, primarily PD-L1, to incapacitate protumor effects and thereby inhibit tumor growth.

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“…3A). Theoretically, the cationexchange resin method may not capture any S. Typhimurium, because the surface of S. Typhimurium was reported to be negatively charged [8]. However, the recovery number of S. Typhimurium after the procedures B, C, and D was significantly decreased from 7.10 log CFU/soil to the range of 4.45-4.72 log CFU/soil (p < 0.05).…”

Section: Determination Of Extraction Methods For S Typhimurium From Soilmentioning

confidence: 95%

Novel Approach of a Phage-Based Magnetoelastic Biosensor for the Detection of Salmonella enterica serovar Typhimurium in Soil

Park

Chin

2016

Journal of Microbiology and Biotechnology

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To date, there has been no employment of a magnetoelastic (ME) biosensor method to detect serovar Typhimurium in soil. The ME biosensor method needs to be investigated and modified for its successful performance. The filtration method, cation-exchange resin method, and combinations of both methods were employed for the extraction of. Typhimurium from soil. The number of . Typhimurium and the resonant frequency shift of the ME sensor were then compared using a brilliant green sulfa agar plate and an HP 8751A network analyzer. A blocking study was performed using bovine serum albumin (BSA), polyethylene glycol (PEG), and casein powder suspension. Finally, the modified ME biosensor method was performed to detect. Typhimurium in soil. The number of . Typhimurium was significantly decreased from 7.10 log CFU/soil to 4.45-4.72 log CFU/soil after introduction of the cation-exchange resin method. The greatest resonant frequency shift of the measurement sensor was found when employing centrifugation and filtration procedures. The resonant frequency shift of the PEG-blocked measurement sensor was 3,219 ± 755 Hz, which was significantly greater than those of the BSA- and casein-blocked ME sensor. The optimum concentration of PEG was determined to be 1.0 mg/ml after considering the resonant shift and economic issue. Finally, the modified ME biosensor method was able to detect. Typhimurium in soil in a dose-response manner. Although these modifications of the ME biosensor method sacrificed some advantages, such as cost, time effectiveness, and operator friendliness, this study demonstrated a novel approach of the ME biosensor method to detect . Typhimurium in soil.

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Salmonella as an Innovative Therapeutic Antitumor Agent

Cited by 52 publications

References 35 publications

Salmonella inhibits tumor angiogenesis by downregulation of vascular endothelial growth factor

Salmonella inhibits tumor angiogenesis by downregulation of vascular endothelial growth factor

Salmonella Breaks Tumor Immune Tolerance by Downregulating Tumor Programmed Death-Ligand 1 Expression

Novel Approach of a Phage-Based Magnetoelastic Biosensor for the Detection of Salmonella enterica serovar Typhimurium in Soil

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