Salmonella and the food industry ‐ methods for isolation, identification and enumeration

Litchfield, John H.; Insalata, N. F.

doi:10.1080/10408397309527149

Cited by 38 publications

(29 citation statements)

References 192 publications

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“…The column was linearly programmed from 160 to 360 C at 4 C/min with an injection port temperature of 340-350 C and 100 ml/min He carrier gas. Comparison of each sample with (11)gave values differing by less than 2%, confirming the general accuracy of the carbon number distributions reported. Since the wax esters were completely eluted from the GLC column before the overlapping triglyceride and diacyl glyceryl ether peaks ( Fig.…”

Section: Analysis Of Intact Lipidssupporting

confidence: 72%

Composition of wax esters, triglycerides and diacyl glyceryl ethers in the jaw and blubber fats of the Amazon River dolphin (Inia geoffrensis)

Ackman¹,

Eaton²,

Litchfield

1971

Lipids

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The lower jaw fat of the Amazon River dolphinInia geoffrensis contains 52.8% wax ester, 44.7% triglyceride and 2.5% diacyl glyceryl ether, while its dorsal blubber fat is >98% triglyceride. Examination of the intact lipids, the derived fatty acids and the derived fatty alcohols by gas chromatography reveals that the blubber triglycerides show characteristics of freshwater fish fats, but the jaw fat lipids have several distinctive features. Jaw fat wax esters, triglycerides and diacyl glyceryl ethers are all rich in C10, C12 and C14 fatty acids and contain no polyunsaturated acids. The fatty alcohols in the wax esters are over 90% saturated. The major carbon numbers in the jaw fat triglycerides (C38–C46) are considerably lower than those of the blubber triglycerides (C48–C54). The possible adaptation of the jaw lipids for use in the underwater echolocation process of this dolphin is discussed.

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Section: Analysis Of Intact Lipidssupporting

confidence: 72%

Composition of wax esters, triglycerides and diacyl glyceryl ethers in the jaw and blubber fats of the Amazon River dolphin (Inia geoffrensis)

Ackman¹,

Eaton²,

Litchfield

1971

Lipids

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“…Posteriormente as amostras foram semeadas em meios semi-sólidos (ágar XLD e Verde Brilhante), onde foi possível visualizar o crescimento de colônias características sugestivas deste gênero que, posteriormente, foram analisadas por testes bioquímicos de acordo com Litchϐield (1973).…”

Section: Methodsunclassified

Microbiologia de swabs retais e otológicos em carnívoros silvestres do zoológico do Parque Estadual de Dois Irmãos, Pernambuco

et al. 2012

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Pesq. Vet. Bras. 32(2):159-164, fevereiro 2012 159 RESUMO.-O estudo epidemiológico de patógenos em populações selvagens no ambiente in situ e ex situ é fundamental para implementação de programas de prevenção, controle e monitoramento de enfermidades e para elabo- The epidemiological study of pathogens in wild populations in the environment in situ and ex situ is essential for implementation of prevention, control and monitoring of diseases and for development of public policies for public and animal health. The study was conducted at the Zoo of Parque Estadual de Dois Irmãos, Recife/PE, Brazil, from January to July 2011, where we collected anal and otological swabs from 29 Zoo's captive wild carnivores. The analysis of the otological swabs showed that 1/29 (3.4%) were positive for Malassezia pachydermatis on direct examination. In the microbiological examination of otologic swabs we found mainly bacteriae of the genus Bacillus 16/29 (55.2%), followed by Staphylococcus 15/29 (51.7%), Escherichia coli 7/29 (24.1%), Streptobacillus 1/29 (3.4%), Micrococcus 1/29 (3.4%) and Klebsiella 1/29 (3.4%). Regarding the presumptive test for the genus Salmonella from rectal swabs, positive samples were observed in six foxes (Cerdocyon thous) and a raccoon (Procyon cancrivorus). The isolation of Salmonella spp. in C. thous and P. cancrivorus indicates risks to public health, especially for zoo professionals who work directly with these animals. This research reinforces the importance of establishing surveillance strategies aimed at prevention, control and monitoring of potential reservoirs of the etiologic agents of infectious and parasitic diseases in the environment of zoos. Microbiologia de INTRODUÇÃOO estudo epidemiológico de patógenos em populações selvagens no ambiente in situ e ex situ é fundamental para implementação de programas de prevenção, controle e monitoramento de enfermidades e para elaboração de políticas públicas de saúde pública e animal (Jorge et al. 2010). Salmonella spp. são bactérias Gram-negativas, móveis, que podem infectar a maioria das espécies de animais, entre mamíferos, aves e répteis, inclusive os humanos, sendo considerada uma zoonose de grande importância fecal--oral, ocorrendo principalmente pelo conta to direto ou indireto com animais infectados ou pela ingestão de alimentos ou água contaminada (Carvalho 2006).A otite é uma enfermidade multifatorial resultante do processo inϐlamatório da cavidade auditiva tendo origem na região externa e podendo prolongar-se até o sistema vestíbulo-coclear. Esse processo migratório geralmente é acompanhado por complicações mais graves e extensas (Greene 1993). Malassezia pachydermatis é uma levedura de natureza oportunista, tornando-se patogênica quando há uma alteração no ambiente microbiológico da superϐície da pele ou no sistema imunológico do hospedeiro levando a um quadro de otite (Mendes et al. 2011). Outras espécies que também podem provocar otite são Malassezia globosa e M. sympodialis (Coutinho et al. 2006).Objetivou-se com este trabalho realizar um...

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“…At this concentration range sulfadiazine inhibits Pseudomonas sp. which grow well on plain BG agar and interfere with Salmonella isolation from feeds and meats (Litchfield 1973). and without 5 pg/ml and 80 pg/ml (Restaino et al 1977) of N, respectively, and BG agar with 8 mg of sulfadiazine/100 ml of medium was compared for isolating salmonellae from various fresh meat products.…”

Section: Introductionmentioning

confidence: 99%

DETERMINATION OF THE EFFECTIVENESS OF NOVOBIOCIN ADDED TO TWO AGAR PLATING MEDIA FOR THE ISOLATION OF SALMONELLA FROM FRESH MEAT PRODUCTS

Komatsu

Restaino

1981

Journal of Food Safety

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Five plating media, Hektoen enteric (HE) and xylose lysine deoxycholate (XLD) agars with and without 80 and 5 μg/ml of novobiocin (N), respectively, and brilliant greeen sulfadiazine (BGS) agar with 80 μg/ml of the antimicrobial agent, were analyzed for the recovery of salmonellae from various fresh beef, pork, and poultry meat products. Of the total Samonella positive samples, 50.0% and 82.5% were found on XLD and XLD‐N agars, respectively, 75.0% and 85.0% on HE and HE‐N agars, respectively and 65.0% on BGS agar. HE‐N and BGS media isolated three times more false positives than did XLD‐N agar, while XLD and HE agars gave the highest numbers of false positives. The major H2S producing false positive on XLD and HE agars was Proteus mirabilis. With the addition of N, P. mirabilis was eliminated, and the major H2S producing false positive was almost exclusively Citrobacter freundii. The false positives on BGS agar were predominately distributed among C. freundii, Enterobacter sp., and Klebsiella sp.

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Salmonella and the food industry ‐ methods for isolation, identification and enumeration

Cited by 38 publications

References 192 publications

Composition of wax esters, triglycerides and diacyl glyceryl ethers in the jaw and blubber fats of the Amazon River dolphin (Inia geoffrensis)

Composition of wax esters, triglycerides and diacyl glyceryl ethers in the jaw and blubber fats of the Amazon River dolphin (Inia geoffrensis)

Microbiologia de swabs retais e otológicos em carnívoros silvestres do zoológico do Parque Estadual de Dois Irmãos, Pernambuco

DETERMINATION OF THE EFFECTIVENESS OF NOVOBIOCIN ADDED TO TWO AGAR PLATING MEDIA FOR THE ISOLATION OF SALMONELLA FROM FRESH MEAT PRODUCTS

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