Sallimus and the Dynamics of Sarcomere Assembly in Drosophila Flight Muscles

Orfanos, Zacharias; Leonard, Kevin; Elliott, Christopher J.H.; Katzemich, Anja; Bullard, Belinda; Sparrow, John C.

doi:10.1016/j.jmb.2015.04.003

Cited by 31 publications

(35 citation statements)

References 19 publications

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“…It has no muscle phenotype heterozygously (Fig 6E), but shows IFM defects homozygously [38]. We first recombined sls ZCL2144 with Df(3R)Exel6176 , a deficiency uncovering the cher locus.…”

Section: Resultsmentioning

confidence: 99%

“…A smaller Z-disc is a very representative phenotype. Apart from cher , it has only been reported for sls [38]. …”

Section: Discussionmentioning

confidence: 99%

“…The following fly stocks were obtained from the Bloomington Drosophila stock center: Mef2-Gal4 , cher CPTI1399 , cher CPTI847 and cher CPTI1403 [31]; cher MI07480-GFSTF.0 , an insertion that integrates a Flag and an EGFP tag [32]; the RNAi transgene cher JF02077 ; the sls ZCL2144 allele (Sls-GFP) is a protein trap that incorporates a His-Tag and a EGFP sequence into all annotated sls transcripts [38]; sls 1 ( D-Titin 14 ) is an EMS amorph mutant and sls j1D7 is a P{lacW} insertion into an exon encoding part of the PEVK-2 domain that fails to complement sls 1 and sls -uncovering deficiencies [56]; the deficiency line Df(3R)Exel6176 uncovers the entire cher locus and was used in combination with all cher alleles. The RNAi transgene cher KK107451 was obtained from the Vienna Drosophila RNAi Center.…”

Section: Methodsmentioning

confidence: 99%

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Filamin actin-binding and titin-binding fulfill distinct functions in Z-disc cohesion

2017

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Many proteins contribute to the contractile properties of muscles, most notably myosin thick filaments, which are anchored at the M-line, and actin thin filaments, which are anchored at the Z-discs that border each sarcomere. In humans, mutations in the actin-binding protein Filamin-C result in myopathies, but the underlying molecular function is not well understood. Here we show using Drosophila indirect flight muscle that the filamin ortholog Cheerio in conjunction with the giant elastic protein titin plays a crucial role in keeping thin filaments stably anchored at the Z-disc. We identify the filamin domains required for interaction with the titin ortholog Sallimus, and we demonstrate a genetic interaction of filamin with titin and actin. Filamin mutants disrupting the actin- or the titin-binding domain display distinct phenotypes, with Z-discs breaking up in parallel or perpendicularly to the myofibril, respectively. Thus, Z-discs require filamin to withstand the strong contractile forces acting on them.

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“…It has no muscle phenotype heterozygously (Fig 6E), but shows IFM defects homozygously [38]. We first recombined sls ZCL2144 with Df(3R)Exel6176 , a deficiency uncovering the cher locus.…”

Section: Resultsmentioning

confidence: 99%

“…A smaller Z-disc is a very representative phenotype. Apart from cher , it has only been reported for sls [38]. …”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Filamin actin-binding and titin-binding fulfill distinct functions in Z-disc cohesion

2017

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“…Gene CG42355 is associated with sperm chromatin condensation, but not much else is presently known. Genes sallimus ( sls ) and zormin have been well documented to be associated with the development of the striated muscle sarcomeres [ 26 – 28 ]. Sls expression is necessary for myoblast fusion, and the inevitable development of myoblasts into muscle fibres.…”

Section: Resultsmentioning

confidence: 99%

“…actin), aiding in balancing the two halves of the sarcomere. Protein zormin can be also be found near the Z-disc and M-line of the muscles [ 28 ]. These filaments connect the Z-disc with the ends of the thick filaments.…”

Section: Resultsmentioning

confidence: 99%

Effects of evolutionary history on genome wide and phenotypic convergence in Drosophila populations

et al. 2018

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BackgroundStudies combining experimental evolution and next-generation sequencing have found that adaptation in sexually reproducing populations is primarily fueled by standing genetic variation. Consequently, the response to selection is rapid and highly repeatable across replicate populations. Some studies suggest that the response to selection is highly repeatable at both the phenotypic and genomic levels, and that evolutionary history has little impact. Other studies suggest that even when the response to selection is repeatable phenotypically, evolutionary history can have significant impacts at the genomic level. Here we test two hypotheses that may explain this discrepancy. Hypothesis 1: Past intense selection reduces evolutionary repeatability at the genomic and phenotypic levels when conditions change. Hypothesis 2: Previous intense selection does not reduce evolutionary repeatability, but other evolutionary mechanisms may. We test these hypotheses using D. melanogaster populations that were subjected to 260 generations of intense selection for desiccation resistance and have since been under relaxed selection for the past 230 generations.ResultsWe find that, with the exception of longevity and to a lesser extent fecundity, 230 generations of relaxed selection has erased the extreme phenotypic differentiation previously found. We also find no signs of genetic fixation, and only limited evidence of genetic differentiation between previously desiccation resistance selected populations and their controls.ConclusionOur findings suggest that evolution in our system is highly repeatable even when populations have been previously subjected to bouts of extreme selection. We therefore conclude that evolutionary repeatability can overcome past bouts of extreme selection in Drosophila experimental evolution, provided experiments are sufficiently long and populations are not inbred.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5118-7) contains supplementary material, which is available to authorized users.

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Microtubules provide guidance cues for myofibril and sarcomere assembly and growth

Dhanyasi

VijayRaghavan

Shilo

et al. 2020

Developmental Dynamics

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Background: Muscle myofibrils and sarcomeres present exceptional examples of highly ordered cytoskeletal filament arrays, whose distinct spatial organization is an essential aspect of muscle cell functionality. We utilized ultrastructural analysis to investigate the assembly of myofibrils and sarcomeres within developing myotubes of the indirect flight musculature of Drosophila. Results: A temporal sequence composed of three major processes was identified: subdivision of the unorganized cytoplasm of nascent, multi-nucleated myotubes into distinct organelle-rich and filament-rich domains; initial organization of the filament-rich domains into myofibrils harboring nascent sarcomeric units; and finally, maturation of the highly-ordered pattern of sarcomeric thick (myosin-based) and thin (microfilament-based) filament arrays in parallel to myofibril radial growth. Significantly, organized microtubule arrays were present throughout these stages and exhibited dynamic changes in their spatial patterns consistent with instructive roles. Genetic manipulations confirm these notions, and imply specific and critical guidance activities of the microtubule-based cytoskeleton, as well as structural interdependence between the myosin-and actinbased filament arrays. Conclusions: Our observations highlight a surprisingly significant, behindthe-scenes role for microtubules in establishment of myofibril and sarcomere spatial patterns and size, and provide a detailed account of the interplay between major cytoskeletal elements in generating these essential contractile myogenic units.

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Sallimus and the Dynamics of Sarcomere Assembly in Drosophila Flight Muscles

Cited by 31 publications

References 19 publications

Filamin actin-binding and titin-binding fulfill distinct functions in Z-disc cohesion

Filamin actin-binding and titin-binding fulfill distinct functions in Z-disc cohesion

Effects of evolutionary history on genome wide and phenotypic convergence in Drosophila populations

Microtubules provide guidance cues for myofibril and sarcomere assembly and growth

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