Salivary pathogen and serum antibody to assess the progression of chronic periodontitis: a 24‐mo prospective multicenter cohort study

Morozumi, Toshiya; Nakagawa, Taneaki; Nomura, Yasuo; Sugaya, Tsutomu; Kawanami, Masamitsu; Suzuki, Fujio; Takahashi, Keiso; Abe, Yuichiro; Sato, Soh; Makino-Oi, Asako; Saitô, Atsushi; Takano, S; Minabe, Masato; Nakayama, Yohei; Ogata, Yorimasa; Kobayashi, Hiroaki; Izumi, Yuichi; Sugano, Naoyuki; Ito, Koichi; Sekino, Satoshi; Numabe, Yukihiro; Fukaya, Chie; Yoshinari, Nobuo; Fukuda, Mitsuo; Noguchi, Toshihide; Kono, Tomoo; Umeda, Makoto; Fujise, O.; Nishimura, Fusanori; Yoshimura, Atsutoshi; Hara, Yoshitaka; Nakamura, Toshiaki; Noguchi, Kazuyuki; Kakuta, Erika; Hanada, Nobuhiro; Takashiba, Shogo; Yoshie, Hiromasa

doi:10.1111/jre.12353

Cited by 32 publications

(30 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of specific antibod ies for oral bacteria in patients with periodontitis indicates an involvement of adaptive immune responses [68], of which different T-cell subsets play a detrimental role in the pathogenesis of this inflammatory disease. The T-cell-associated cytokine profile in gingival tissue suggests an engagement of T-helper (Th) 1, Th2, and Th17 cells [69][70][71].…”

Section: Leukocytesmentioning

confidence: 99%

Cellular Response Mechanisms in Porphyromonas gingivalis Infection

Khalaf¹,

Palm²,

Bengtsson³

2017

Periodontitis - A Useful Reference

View full text Add to dashboard Cite

The pathogenicity of the periodontal biofilm is highly dependent on a few key species, of which Porphyromonas gingivalis is considered to be one of the most important pathogens.P. gingivalis expresses a broad range of virulence factors, of these cysteine proteases (gin gipains) are of special importance both for the bacterial survival/proliferation and for the pathological outcome. Several cell types, for example, epithelial cells, endothelial cells, dendritic cells, osteoblasts, and fibroblasts, reside in the periodontium and are part of the innate host response, as well as platelets, neutrophils, lymphocytes, and monocytes/mac rophages. These cells recognize and respond to P. gingivalis and its components through pattern recognition receptors (PRRs), for example, Toll-like receptors and protease-acti vated receptors. Ligation of PRRs induces downstream-signaling pathways modifying the activity of transcription factors that regulates the expression of genes linked to inflamma tion. This is followed by the release of inflammatory mediators, for example, cytokines and reactive oxygen species. Periodontal disease is today considered to play a significant role in various systemic conditions such as cardiovascular disease (CVD). The mechanisms by which P. gingivalis and its virulence factors interact with host immune cells and contribute to the pathogenesis of periodontitis and CVD are far from completely understood.

show abstract

Section: Leukocytesmentioning

confidence: 99%

Cellular Response Mechanisms in Porphyromonas gingivalis Infection

Khalaf¹,

Palm²,

Bengtsson³

2017

Periodontitis - A Useful Reference

View full text Add to dashboard Cite

show abstract

“…Each sample thus collected was centrifuged at 3,000 rpm for 5 min at 4ºC. All the samples were collected in the order described above and then immediately sent to a medical examination laboratory (BML Corporation, Tokyo, Japan) for bacterial analysis (7)(8)(9)12).…”

Section: Sample Collectionmentioning

confidence: 99%

“…Briefly, bacterial DNA was extracted using a commercial kit (MagNA Pure LC Total Nucleic Acid Isolation Kit, Roche, Basel, Switzerland). Primary probes and Invader oligos were used as described previously (7,12). Each tube contained 15 μL of reaction mixture containing 50 μM dNTP, 700 nM primary probe, 70 nM Invader oligo, 2.5 U polymerase chain reaction (PCR) enzyme (EagleTaq DNA polymerase, Roche), and the Invader core reagent kit (Cleavase XI Invader core reagent kit, HOLOGIC, Marlborough, MA, USA) containing a fluorescence resonance energy transfer (FRET) mix, an enzyme/MgCl 2 solution and template DNA.…”

Section: Sample Collectionmentioning

confidence: 99%

“…Quantitative analysis of periodontal bacteria in clinical specimens, such as saliva, supragingival plaque and subgingival plaque, is important for diagnosis, evaluation, and risk assessment of patients with periodontal disease or who are prone to it. Using real-time PCR methods (5-7), clinicians can easily evaluate these periodontal bacteria and their association with clinical symptoms (8)(9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of specimens obtained by different sampling methods for evaluation of periodontal bacteria

Okada

Sogabe

Takeuchi

et al. 2017

Journal of Oral Science

View full text Add to dashboard Cite

Quantitative analysis of periodontal bacteria is considered useful for clinical diagnosis, evaluation and assessment of the risk of periodontal disease. The purpose of this study was to compare the effectiveness of sampling of saliva, supragingival and subgingival plaque for evaluation of periodontal bacteria. From each of 12 subjects, i) subgingival plaque was collected from the deepest pocket using a sterile paper point, ii) stimulated whole saliva was collected after chewing gum, and iii) supragingival plaque was collected using a tooth brush. These samples were sent to the medical examination laboratory for quantitative analysis of the counts of three periodontal bacterial species: Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia. The proportions of these bacteria in subgingival plaque were higher than those in saliva or supragingival plaque, but lower in subgingival plaque than in saliva or supragingival plaque. In several cases, periodontal bacteria were below the levels of detection in subgingival plaque. We concluded that samples taken from subgingival plaque may be more useful for evaluating the proportion of periodontal bacteria in deep pockets than is the case for other samples. Therefore, for evaluation of periodontal bacteria, clinicians should consider the characteristics of the specimens obtained using different sampling methods.

show abstract

“…It is hypothesized that these pathogens would be able to mediate the microbial community’s conversion into dysbiosis, and a wide perturbation of this community would cause and/or sustain the process of periodontal breakdown 20 . In a recent multicenter clinical study in Japan, it was suggested that the combination of Pg ratio in saliva and serum immunoglobulin G (IgG) titers against Pg may be associated with progression of periodontitis 21 . Imamura et al 22 previously reported on a novel immunochromatographic device (DK13‐PG‐001) for rapid and accurate clinical detection of Pg in subgingival plaque.…”

mentioning

confidence: 99%

Clinical Usefulness of Novel Immunochromatographic Detection Device for Porphyromonas gingivalis in Evaluating Effects of Scaling and Root Planing and Local Antimicrobial Therapy

Nakayama

Ogata

Hiromatsu

et al. 2016

Journal of Periodontology

View full text Add to dashboard Cite

show abstract

Salivary pathogen and serum antibody to assess the progression of chronic periodontitis: a 24‐mo prospective multicenter cohort study

Abstract: It is suggested that the combination of P. gingivalis ratio in saliva and serum IgG titers against P. gingivalis may be associated with the progression of periodontitis.

Cited by 32 publications

References 42 publications

Cellular Response Mechanisms in Porphyromonas gingivalis Infection

Cellular Response Mechanisms in Porphyromonas gingivalis Infection

Characterization of specimens obtained by different sampling methods for evaluation of periodontal bacteria

Clinical Usefulness of Novel Immunochromatographic Detection Device for Porphyromonas gingivalis in Evaluating Effects of Scaling and Root Planing and Local Antimicrobial Therapy

Contact Info

Product

Resources

About