Salivary microRNAs as new molecular markers in cleft lip and palate: a new frontier in molecular medicine

Grassia, Vincenzo; Lombardi, Angela; Kawasaki, Hiromichi; Ferri, Carmela; Perillo, Letizia; Mosca, Laura; Cave, Donatella Delle; Nucci, Ludovica; Porcelli, Marina; Caraglia, Michele

doi:10.18632/oncotarget.24838

Cited by 32 publications

(33 citation statements)

References 38 publications

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“…Nowadays, the growing threat of microbial resistance against traditional antibiotics in oral disease has prompted the mandatory development of alternative therapies, especially against bacterial strains showing resistance to one or more antibiotics [60,61]. In this article, silver NPs (AgNPs) were successfully prepared using hydrazine and were further modified by a coating of indolicidin, a well-known AMP.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Activity of Indolicidin-Coated Silver Nanoparticles in Oral Disease

et al. 2020

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Featured Application: Indolicidin-coated silver nanoparticles could be used as platforms in the dental field as oral disease preventive drug. Furthermore, nanomaterials could prevent oral cancers and balance oral health care. Abstract:(1) Background: In dentistry, silver nanoparticles (AgNPs) have progressively earned great interest as antimicrobial drugs and are widely used in several biomedical fields. Recent progress in the analysis of complex bacterial communities has demonstrated the richness of the oral microbiota and the presence of numerous previously unexplained strains. Several efforts have been dedicated to the investigation of antimicrobial peptides (AMPs). Those peptides are a widespread group of small peptides against invading microbes. We report the production of a hybrid molecule composed of AgNPs and indolicidin, a well-known antibacterial peptide. (2) Methods: Spectroscopy and microscopy were used to analyze the optical features and to determine the size of the generated AgNPs. The AgNP antibacterial activity was evaluated versus oral Gram-positive and Gram-negative bacteria.(3) Results: The coated nanoparticles' antibacterial activity strongly inhibited the growth of microorganisms, with very low minimum inhibitory concentration (MIC) values in the range of 5-12.5 µg/mL. We hypothesize that this effect depended on the specific characteristics of the metal surface coated with indolicidin. The second result was that the coated nanoparticles observed cellular toxicity, was lower with respect to the toxicity of peptide and the naked AgNPs when used individually. (4) New investigations regarding antimicrobial effect of AgNPs coated with AMPs in oral infections are an urgent task.

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Section: Discussionmentioning

confidence: 99%

Antibacterial Activity of Indolicidin-Coated Silver Nanoparticles in Oral Disease

et al. 2020

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“…Moreover, in extraction treatments, the arch width reduction creates unaesthetic triangles at the corners of the mouth with negative spaces lateral to the buccal segments [32] and undesirable dental retraction; worsening facial aesthetics are possible [6]. Thus, the trend of nonextraction treatment has changed due to the new aesthetic concepts of facial soft-tissue profile in orthodontics [32][33][34][35][36]. The present case shows a patient with a severe mandibular crowding, treated without extractions and with a postretention long-term stability (10-year follow-up).…”

Section: Discussionmentioning

confidence: 99%

Long-Term Outcomes of Nonextraction Treatment in a Patient with Severe Mandibular Crowding

Grassia

Nucci

Marra

et al. 2020

Case Reports in Dentistry

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Objective. To describe a clinical case with a severe mandibular crowding treated without extraction and showing a long-term outcome. Methods. A 14-year-old boy in permanent dentition showed a class I molar and cuspid relationship, a severe deep bite of 8 mm, a constricted V-shaped upper arch with moderate crowding, and a severe crowding of about 12 mm in the lower arch. The panoramic X-ray showed an impacted upper right canine. The treatment started with the placement of a transpalatal bar and 0.022×0.028 in standard edgewise appliances in the upper arch and a lip bumper bonded on the second lower molars. Initial leveling of the teeth was accomplished with light Australian round wires. Finishing was then performed with rectangular wires. The phase with fixed appliances lasted 2 years and 9 months, and the patient was motivated and cooperative throughout the treatment, although with poor oral hygiene. The patient was treated without extractions. Results. The space was gained with the first and second upper molar derotations using the transpalatal bar and the gingival lip bumper in the lower arch. The upper right canine was well positioned, and the maxillary arch form was improved. The severe lower crowding was completely corrected, and a good overbite was achieved. Conclusion. A conservative, nonextraction treatment approach for this patient with class I malocclusion with severe mandibular crowding was effective, and the results have remained stable after a long-term follow-up (10 years).

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“…Many different microRNAs have been identified to temporally and spatially regulate morphogens and transcription factors during palatogenesis (Eberhart et al, 2008;Seelan et al, 2014;Ding et al, 2016;Reiss and Bhakdi, 2017;Schoen et al, 2017). Not surprisingly, microRNAs have been suggested as be new targets for investigating in CL/P studies (Li et al, 2010;Wang et al, 2013Wang et al, , 2017Ma et al, 2014;Gao et al, 2015;Li D. et al, 2016;Li J. et al, 2016;Schoen et al, 2017Schoen et al, , 2018Chen et al, 2018;Grassia et al, 2018;Pan et al, 2018;Suzuki et al, 2018;Wu N. et al, 2018;Xu M. et al, 2018).…”

Section: Ecm Structural Molecules and Soluble Factorsmentioning

confidence: 99%

Extracellular Matrix Composition and Remodeling: Current Perspectives on Secondary Palate Formation, Cleft Lip/Palate, and Palatal Reconstruction

Paiva

Maas

Santos

et al. 2019

Front. Cell Dev. Biol.

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Craniofacial development comprises a complex process in humans in which failures or disturbances frequently lead to congenital anomalies. Cleft lip with/without palate (CL/P) is a common congenital anomaly that occurs due to variations in craniofacial development genes, and may occur as part of a syndrome, or more commonly in isolated forms (non-syndromic). The etiology of CL/P is multifactorial with genes, environmental factors, and their potential interactions contributing to the condition. Rehabilitation of CL/P patients requires a multidisciplinary team to perform the multiple surgical, dental, and psychological interventions required throughout the patient's life. Despite progress, lip/palatal reconstruction is still a major treatment challenge. Genetic mutations and polymorphisms in several genes, including extracellular matrix (ECM) genes, soluble factors, and enzymes responsible for ECM remodeling (e.g., metalloproteinases), have been suggested to play a role in the etiology of CL/P; hence, these may be considered likely targets for the development of new preventive and/or therapeutic strategies. In this context, investigations are being conducted on new therapeutic approaches based on tissue bioengineering, associating stem cells with biomaterials, signaling molecules, and innovative technologies. In this review, we discuss the role of genes involved in ECM composition and remodeling during secondary palate formation and pathogenesis and genetic etiology of CL/P. We also discuss potential therapeutic approaches using bioactive molecules and principles of tissue bioengineering for state-of-the-art CL/P repair and palatal reconstruction.

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Salivary microRNAs as new molecular markers in cleft lip and palate: a new frontier in molecular medicine

Cited by 32 publications

References 38 publications

Antibacterial Activity of Indolicidin-Coated Silver Nanoparticles in Oral Disease

Antibacterial Activity of Indolicidin-Coated Silver Nanoparticles in Oral Disease

Long-Term Outcomes of Nonextraction Treatment in a Patient with Severe Mandibular Crowding

Extracellular Matrix Composition and Remodeling: Current Perspectives on Secondary Palate Formation, Cleft Lip/Palate, and Palatal Reconstruction

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