Salivary Microrna As a Biomarker for Monitoring Response to Treatment in Eosinophilic Esophagitis

Kelbel, Theodore; Ghaffari, Gisoo; Sena, Maria Helena Lima Gusmão; Ishmael, Faoud T.

doi:10.1016/j.jaci.2014.12.1187

Cited by 4 publications

(5 citation statements)

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“…A resulting deficiency of SPINK7 can lead to increased damage of the esophageal epithelium in EoE 5p in EoE samples compared to control. Expression of miR-3613-5p and miR-4668-5p decreased in patients following fluticasone treatment, suggesting these two miRNAs may be candidate biomarkers for surveilling treatment response [40] . Similarly, Swanson et al [41] profiled salivary miRNA in a pediatric cohort and discovered 97 differentially expressed miRNAs, with miR-223 having the greatest level of expression.…”

Section: Extracellular Mirnas [Plasma and Salivary Mirnas] As Noninvamentioning

confidence: 99%

“…Extracellular miRNAs are detectable in the oronasaopharyngeal cavity and can also be obtained non-invasively without endoscopy. Although limited, a few recent pilot studies hint at the potential to reliably detect different miRNAs signatures in saliva of EoE patients vs. controls [40,41] . Kelbel et al [40] analyzed miRNA expression in saliva samples from 15 adult patients with EoE and 17 healthy controls.…”

Section: Extracellular Mirnas [Plasma and Salivary Mirnas] As Noninvamentioning

confidence: 99%

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Biomarkers and therapeutic targets: microRNA roles in the pathophysiology, diagnosis and management of eosinophilic esophagitis

Lambert

Jhaveri²,

Jhaveri

2018

JTGG

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How to cite this article: Lambert KA, Jhaveri P, Jhaveri P. Biomarkers and therapeutic targets: microRNA roles in the pathophysiology, AbstractEosinophilic esophagitis (EoE) is a chronic inflammatory disease that is increasingly recognized as the cause of common gastrointestinal symptoms including dysphagia, chest and abdominal pain, heartburn, food impaction, and food refusal in children and adults. Often referred to as "asthma of the esophagus", eosinophilic esophagitis, like its asthma counterpart, is an allergic disorder on the rise worldwide. Clinically managed by food avoidance, steroid therapy, recurring endoscopic evaluations and dilations as needed, eosinophilic esophagitis is a poorly understood disease process with limited therapies and even fewer diagnostic tools to predict and surveil active inflammation. As a result, there is a critical need to identify noninvasive biomarkers and therapeutic targets for eosinophilic esophagitis. Here we review the known contributions of miRNAs to eosinophilic inflammation of the esophagus and the potential for miRNA biomarkers for EoE in the clinical setting.

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Section: Extracellular Mirnas [Plasma and Salivary Mirnas] As Noninvamentioning

confidence: 99%

Section: Extracellular Mirnas [Plasma and Salivary Mirnas] As Noninvamentioning

confidence: 99%

Biomarkers and therapeutic targets: microRNA roles in the pathophysiology, diagnosis and management of eosinophilic esophagitis

Lambert

Jhaveri²,

Jhaveri

2018

JTGG

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“…We previously utilized a qPCR array to identify a panel of candidate miRNAs whose salivary expression was different in subjects with EoE vs. those without. 15 MiR-4668 emerged as a novel target, as it has not previously been studied in allergic disease. To further characterize its potential as a biomarker in EoE, we measured its expression in an expanded cohort of subjects in this study.…”

Section: Associations Between Baseline Mirna Expression and Clinical mentioning

confidence: 99%

“…We identified a set of candidate miRNA biomarkers and found increased levels of miR-570-3p, miR-3613-5p, miR-4668-5p, and miR-30a-5p in EoE samples compared to control. 15 Expression of miR-3613-5p and miR-4668-5p decreased in patients following fluticasone treatment, suggesting these two miRNAs may be candidate biomarkers for surveilling treatment response.…”

Section: Introductionmentioning

confidence: 98%

MiR-4668 as a Novel Potential Biomarker for Eosinophilic Esophagitis

Bhardwaj

Sena

Ghaffari

et al. 2020

Allergy�Rhinol (Providence)

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Introduction Eosinophilic esophagitis (EoE) is a clinico-pathological diagnosis characterized by esophageal dysfunction and eosinophilic infiltration of the esophagus. Demonstration of esophageal eosinophilia (more than 15 eosinophils/hpf) in biopsy specimen obtained by esophagogastroduodenoscopy (EGD) continues to be the gold standard for diagnosis and monitoring of response to therapy. There is a growing necessity for non-invasive biomarkers that can accurately diagnose this condition and assess response to therapy. While microRNAs (miRNA) are being investigated in allergic diseases, including EoE, not many studies have explored the role of salivary miRNAs in EoE. MiR-4668-5p is a particularly interesting candidate, as it is predicted to regulate TGF-beta signaling and has not previously been identified as a target in any allergy disease. We sought to further investigate the role of miR-4668 as a biomarker to characterize and monitor response to treatment with swallowed topical glucocorticoids. Methods After IRB approval, twenty-two adult patients with EoE were randomly enrolled to provide a saliva sample before and after 2 months of swallowed fluticasone therapy. Differences of miRNA expression before and after treatment were analyzed by paired T-test. A significance cutoff of <0.05 was used for all analyses. Results Expression of miR-4668 was higher in EoE vs. non-EoE subjects. The level of miR-4668 decreased in all subjects except one, with a mean fold change 0.49 ± 0.25. There was an association between miRNA expression and number of positive aeroallergens. The miR-4668 high group had a higher number of positive aeroallergen tests, while the miR-4668 low group had a greater number of subjects with drug allergies. Conclusions In this study, we identified that salivary miRNAs may serve as biomarkers to characterize EoE and response to topical corticosteroids. We specifically identified miR-4668 as a novel potential biomarker, which was not previously discovered as a target in EoE or any other allergic disease.

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“…Pilot studies also have indicated massive dysregulation of salivary microRNAome in EoE when compared with healthy controls, with the potential to serve as noninvasive markers for identifying and monitoring response to treatment in EoE. [51][52][53] Exhaled Nitric Oxide Exhaled nitric oxide has been examined as a noninvasive marker of airway inflammation. In a proofof-concept study, normal levels of exhaled nitric oxide had good specificity for screening children with EoE without airway inflammation.…”

Section: Salivamentioning

confidence: 99%

Promising Modalities to Identify and Monitor Eosinophilic Esophagitis

Hiremath

Gupta

2017

Clinical Gastroenterology and Hepatology

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Eosinophilic esophagitis (EoE) is an allergen-mediated condition characterized by symptoms of esophageal dysfunction and histologic evidence of intense eosinophilic inflammation involving the esophagus in the absence of overlapping conditions such as gastroesophageal reflux disease. Since the initial description as a distinct entity approximately 2 decades ago, there has been a remarkable increase in the recognition of this clinicopathologic entity. The current approach to diagnose and monitor EoE requires repeated esophagogastroduodenoscopies, with associated sedation/anesthesia, to visualize mucosal abnormalities, and to obtain multiple biopsy specimens for histologic assessment and to evaluate treatment response. Frequent esophagogastroduodenoscopies with multiple biopsies can increase the risk of procedural complications, place significant financial burden on families, and escalate health care costs. In addition, this burdensome approach may contribute toward delayed diagnosis and suboptimal monitoring, thereby increasing the likelihood of complications such as esophageal narrowing and stricture formation, which may require escalation of care including endoscopic interventions. Clinical progression and complications associated with EoE can be attenuated through early identification and optimal management. Therefore, developing reliable, safe, less-cumbersome, and cost-effective modalities for early diagnosis and effective monitoring of EoE is an area of active research. These efforts have been substantially supported by the development of new biomaterials, analytic methodologies, and the application of novel concepts. Herein, we summarize modalities that have shown promise to advance the diagnosis and monitoring of EoE and could improve the care of affected individuals and advance the field.

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Salivary Microrna As a Biomarker for Monitoring Response to Treatment in Eosinophilic Esophagitis

Cited by 4 publications

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Biomarkers and therapeutic targets: microRNA roles in the pathophysiology, diagnosis and management of eosinophilic esophagitis

Biomarkers and therapeutic targets: microRNA roles in the pathophysiology, diagnosis and management of eosinophilic esophagitis

MiR-4668 as a Novel Potential Biomarker for Eosinophilic Esophagitis

Promising Modalities to Identify and Monitor Eosinophilic Esophagitis

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