Salivary metabolite signatures of oral cancer and leukoplakia through gas chromatography-mass spectrometry

Tantray, Shoborose; Sharma, S. Subash; Prabhat, Kanika; Nasrullah, Nazima; Gupta, Manu

doi:10.4103/jomfp.jomfp_335_21

Cited by 7 publications

(3 citation statements)

References 12 publications

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“…However, this has been a minor obstacle in metabolomics studies since the detection methods are highly sensitive [ 146 ]. Given the richness and metabolic response it provides, saliva samples have been used in screening for diagnosis and monitoring T2DM [ 148 , 149 , 150 ], neurological diseases (such as Alzheimer’s [ 151 ] and schizophrenia [ 152 ]), and some types of cancers (such as oral, breast, cerebral, colorectal, and gastric cancers) [ 153 , 154 , 155 ]. It is the ideal biofluid for studying oral diseases including periodontitis [ 156 , 157 ] and dental caries [ 158 , 159 ].…”

Section: Underexplored Specimens In Clinical Metabolomicsmentioning

confidence: 99%

Critical Factors in Sample Collection and Preparation for Clinical Metabolomics of Underexplored Biological Specimens

de Souza,

Pereira,

de Sá

et al. 2024

Metabolites

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This review article compiles critical pre-analytical factors for sample collection and extraction of eight uncommon or underexplored biological specimens (human breast milk, ocular fluids, sebum, seminal plasma, sweat, hair, saliva, and cerebrospinal fluid) under the perspective of clinical metabolomics. These samples are interesting for metabolomics studies as they reflect the status of living organisms and can be applied for diagnostic purposes and biomarker discovery. Pre-collection and collection procedures are critical, requiring protocols to be standardized to avoid contamination and bias. Such procedures must consider cleaning the collection area, sample stimulation, diet, and food and drug intake, among other factors that impact the lack of homogeneity of the sample group. Precipitation of proteins and removal of salts and cell debris are the most used sample preparation procedures. This review intends to provide a global view of the practical aspects that most impact results, serving as a starting point for the designing of metabolomic experiments.

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Section: Underexplored Specimens In Clinical Metabolomicsmentioning

confidence: 99%

Critical Factors in Sample Collection and Preparation for Clinical Metabolomics of Underexplored Biological Specimens

de Souza,

Pereira,

de Sá

et al. 2024

Metabolites

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“…Several studies, with different protocols, conducted over the last few years identified different saliva metabolite concentrations either between healthy controls and oral squamous cell carcinoma subjects or between OSCC subjects and premalignant lesion subjects (oral lichen planus, oral leukoplakia, precancerous dysplasia, keratosis). Their samples, analytical and discrimination methods and outcomes are summarized in Table 1 [ 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 ]. A notable diversity of candidate biomarkers is presented in these studies.…”

Section: The Human Salivary Metabolome and Oral Pathologiesmentioning

confidence: 99%

“…Choline is also found among other metabolites as a potential biomarker in the research of Ohshima et al, in 2017 [ 60 ]. Glycine, proline and ornithine were found in three independent studies by Lohavanichbutr et al (2018), Ishakawa et al (2019) and Tantray et al (2022) as potential oral cancer biomarkers [ 61 , 62 , 67 ]. However, different studies identified different groups of metabolites that were either upregulated or downregulated in OSCC and precancerous samples compared to healthy controls.…”

Section: The Human Salivary Metabolome and Oral Pathologiesmentioning

confidence: 99%

Saliva Metabolomic Profile in Dental Medicine Research: A Narrative Review

Tzimas

Pappa

2023

Metabolites

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Metabolomic research tends to increase in popularity over the years, leading to the identification of new biomarkers related to specific health disorders. Saliva is one of the most newly introduced and systematically developed biofluids in the human body that can serve as an informative substance in the metabolomic profiling armamentarium. This review aims to analyze the current knowledge regarding the human salivary metabolome, its alterations due to physiological, environmental and external factors, as well as the limitations and drawbacks presented in the most recent research conducted, focusing on pre—analytical and analytical workflows. Furthermore, the use of the saliva metabolomic profile as a promising biomarker for several oral pathologies, such as oral cancer and periodontitis will be investigated.

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