Salivary Glyco-sialylation changes monitors oral carcinogenesis

Vajaria, Bhairavi N.; Patel, Kinjal D.; Begum, Rasheedunnisa; Patel, J. D.; Shah, Franky D.; Joshi, Geeta; Patel, Prabhudas S.

doi:10.1007/s10719-014-9561-7

Cited by 15 publications

(22 citation statements)

References 33 publications

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“…In this study, we investigated the mRNA levels of various STs (ST3GAL1, ST3GAL2, ST3GAL3, ST3GAL4, ST3GAL6, and ST6GAL1) and sialidases (NEU3) and found that all the transcripts were significantly down‐regulated in malignant tissues as compared to normal tissues. Although in our previous reports, we found increased levels of STs and sialidase enzyme activity in malignant tissue, serum, and saliva of oral cancer patients . However, in this study the non‐malignant and microscopically uninvolved part of tumor showed enhanced mRNA levels of STs and sialidase.…”

Section: Discussioncontrasting

confidence: 89%

“…Carbohydrate determinants present on the cancer cell surface contain sialylated and/or fucosylated structures. Synthesis of these carbohydrate determinants is regulated by a set of STs and fucosyltransferases (FUTs) . Hypersialylation is often found on cell surface glycoproteins of malignant cells and also found to be associated with the malignant phenotype and metastatic potential of different cancers .…”

Section: Discussionmentioning

confidence: 99%

“…Expressions of STs are often de-regulated in various cancers like colorectal, liver, gliomas, and gastric. 5,9,10 We have previously reported association of higher STs and sialidase enzyme activity in malignant tissue, serum, and saliva with disease progression and treatment monitoring in oral cancer. 11 The development of newer drugs targeting different interplays of sialylation might have profound effect in inhibiting sialylation and thus cancer progression, metastasis, and infiltrations.…”

Section: Studies On Sialylation Pattern In Oral Cancer Are Clinicalmentioning

confidence: 99%

“…Various types of STs are involved in the formation of different types of sialylated tumor‐associated carbohydrate antigens which plays important role in progression and metastasis. Expressions of STs are often de‐regulated in various cancers like colorectal, liver, gliomas, and gastric . We have previously reported association of higher STs and sialidase enzyme activity in malignant tissue, serum, and saliva with disease progression and treatment monitoring in oral cancer .…”

Section: Introductionmentioning

confidence: 98%

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“Aberrant sialylation plays a significant role in oral squamous cell carcinoma progression”

Mehta

Patel

Pandya

et al. 2020

J Oral Pathology Medicine

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Objective Aberrant glycosylation, mainly sialylation and fucosylation, is recently considered as a major hallmark of cancer. Aberrant sialylation has long been associated with various neoplastic diseases. However, role of aberrant sialylation in oral cancer is still in its infancy. The present study aimed to examine mRNA expressions of α‐2, 3, α‐2, 6 sialyltransferase (ST) families and sialidase in 160 human oral cancer tissues. Methods mRNA expression of ST3GAL1, ST3GAL2, ST3GAL3, ST3GAL4, ST3GAL6, ST6GAL1, and neuraminidase 3 (NEU3) was analyzed by RT‐qPCR in 80 paired malignant and adjacent normal tissues from oral cancer patients. Results The results indicated significant (P ≤ .05) down‐regulation of various STs (ST3GAL1, ST3GAL2, ST3GAL3, ST3GAL4, ST3GAL6, and ST6GAL1) and sialidases (NEU3) in malignant tissues as compared to adjacent normal tissues. Higher mRNA levels of ST3GAL2 and ST3GAL3 were significantly associated with advanced stage of the disease, lymph node involvement, and perineural invasion, which denote their role in progression and metastasis of oral cancer. Present study also revealed altered sialylation patterns according to anatomical site of the disease and tobacco habit. Conclusion The study demonstrated significant role of elevated mRNA levels of ST3GAL2 and ST3GAL3 in disease progression and metastasis of oral carcinoma.

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Section: Discussioncontrasting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Studies On Sialylation Pattern In Oral Cancer Are Clinicalmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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“Aberrant sialylation plays a significant role in oral squamous cell carcinoma progression”

Mehta

Patel

Pandya

et al. 2020

J Oral Pathology Medicine

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“…[25] Similarly, Vajaria et al reported on salivary sialylation changes where the levels of sialic acid were increased in precancerous and oral cancer. [26] Sanjay et al reported a higher level of salivary total protein, total sugar, and sialic acid in OSCC when compared to normal healthy individuals. According to their study, Salivary sialic acid levels were significantly elevated in well-differentiated squamous cell carcinomas than in moderately differentiated squamous cell carcinomas.…”

Section: Discussionmentioning

confidence: 99%

Estimation of salivary sialic acid levels as a prognostic marker in patients with oral leukoplakia - A case control study

Yoga¹,

Rangdhol²,

Maithilikarpagaselvi³

et al. 2017

JCRI

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Background: Oral leukoplakia (OL) is a potentially malignant disorder seen in tobacco users. Salivary biomarkers such as salivary sialic acid which is significantly high in OL and oral cancer serves as a potent biomarker for early diagnosis thereby improving prognosis. Aim: To estimate and compare salivary sialic acid levels in tobacco users with and without OL. Methods: The study included 172 tobacco users reporting to Private Dental Teaching Hospital, aged between 20 and 70 years, selected according to inclusion and exclusion criteria and divided into two groups. Study group included 76 patients with OL and control group, 96 patients without OL. Unstimulated whole saliva was collected and salivary sialic acid levels were estimated and the results were subjected to statistical analysis (unpaired students t-test). Results: The mean range of salivary sialic acid in controls was 1.898 ± 0.289 µmol/L and cases was 2.680 ± 0.189 µmol/L. Salivary sialic levels are high in cases when compared with controls with a statistically significant P < 0.001*. Conclusion:The results of our study clearly indicate that salivary sialic acid levels are significantly raised in patients with OL when compared with patients without OL. With the observations of our study salivary sialic acid proves to be a sensitive, specific, and cost-effective biomarker for OL.

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Head and neck cancer N-glycome traits are cell line and HPV status–dependent

et al. 2022

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Glycosylation is the most common post-translational modification of proteins, and glycosylation changes at cell surfaces are frequently associated with malignant epithelia including head and neck squamous cell carcinoma (HNSCC). In HNSCC, 5-year survival remains poor, averaging around 50% globally: this is partly related to late diagnosis. Specific protein glycosylation signatures on malignant keratinocytes have promise as diagnostic and prognostic biomarkers and as therapeutic targets. Nevertheless, HNSCC-specific glycome is to date largely unknown. Herein, we tested six established HNSCC cell lines to capture the qualitative and semi-quantitative N-glycome using porous graphitized carbon liquid chromatography coupled to electrospray ionisation tandem mass spectrometry. Oligomannose-type N-glycans were the predominant features in all HNSCC cell lines analysed (57.5–70%). The levels of sialylated N-glycans showed considerable cell line-dependent differences ranging from 24 to 35%. Importantly, α2-6 linked sialylated N-glycans were dominant across most HNSCC cell lines except in SCC-9 cells where similar levels of α2-6 and α2-3 sialylated N-glycans were observed. Furthermore, we found that HPV-positive cell lines contained higher levels of phosphorylated oligomannose N-glycans, which hint towards an upregulation of lysosomal pathways. Almost all fucose-type N-glycans carried core-fucose residues with just minor levels (< 4%) of Lewis-type fucosylation identified. We also observed paucimannose-type N-glycans (2–5.5%), though in low levels. Finally, we identified oligomannose N-glycans carrying core-fucose residues and confirmed their structure by tandem mass spectrometry. This first systematic mapping of the N-glycome revealed diverse and specific glycosylation features in HNSCC, paving the way for further studies aimed at assessing their possible diagnostic relevance.

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Salivary Glyco-sialylation changes monitors oral carcinogenesis

Cited by 15 publications

References 33 publications

“Aberrant sialylation plays a significant role in oral squamous cell carcinoma progression”

“Aberrant sialylation plays a significant role in oral squamous cell carcinoma progression”

Estimation of salivary sialic acid levels as a prognostic marker in patients with oral leukoplakia - A case control study

Head and neck cancer N-glycome traits are cell line and HPV status–dependent

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