Salivary Glands after Prolonged Aluminum Exposure: Proteomic Approach Underlying Biochemical and Morphological Impairments in Rats

Souza-Monteiro, Deiweson; M, Guerra; Bittencourt, Leonardo Oliveira; Aragão, Walessa Alana Bragança; Dionízio, Aline; Silveira, Felipe Martins; Buzalaf, Marilia A. R.; Martins, Manoela Domingues; Crespo-López, María Elena; Lima, Rafael Rodrigues

doi:10.3390/ijms23042251

Cited by 7 publications

(15 citation statements)

References 67 publications

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“…Studies suggest that the incidence of AD increased significantly in the elderly after prolonged ingestion of water with high concentrations of Al [ 14 ]. The possible molecular mechanisms involved in the Al-induced toxicity process are oxidative stress, cytotoxicity, genotoxicity, pro-inflammatory effects, enzyme dysfunctions, metabolic disturbances, membrane disruption, microtubule disruption, necrosis, and apoptosis [ 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Global Proteomic Profile of Aluminum-Induced Hippocampal Impairments in Rats: Are Low Doses of Aluminum Really Safe?

Bittencourt

Silva

Aragão

et al. 2022

IJMS

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Hippocampus is the brain area where aluminum (Al) accumulates in abundance and is widely associated with learning and memory. In the present study, we evaluate behavioral, tissue, and proteomic changes in the hippocampus of Wistar rats caused by exposure to doses that mimic human consumption of aluminum chloride (AlCl3) in urban areas. For this, male Wistar rats were divided into two groups: Control (distilled water) and AlCl3 (8.3 mg/kg/day), both groups were exposed orally for 60 days. After the Al exposure protocol, cognitive functions were assessed by the Water maze test, followed by a collection for analysis of the global proteomic profile of the hippocampus by mass spectrometry. Aside from proteomic analysis, we performed a histological analysis of the hippocampus, to the determination of cell body density by cresyl violet staining in Cornu Ammonis fields (CA) 1 and 3, and hilus regions. Our results indicated that exposure to low doses of aluminum chloride triggered a decreased cognitive performance in learning and memory, being associated with the deregulation of proteins expression, mainly those related to the regulation of the cytoskeleton, cellular metabolism, mitochondrial activity, redox regulation, nervous system regulation, and synaptic signaling, reduced cell body density in CA1, CA3, and hilus.

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Section: Introductionmentioning

confidence: 99%

Global Proteomic Profile of Aluminum-Induced Hippocampal Impairments in Rats: Are Low Doses of Aluminum Really Safe?

Bittencourt

Silva

Aragão

et al. 2022

IJMS

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“…Out of 1756 articles, 26 articles regarding the effect of different substances on salivary glands were included in the analysis ( Figure 1 ). Their distribution was: 6 articles on the effect of chemotherapy drugs on salivary glands [ 5 , 12 , 15 , 16 , 18 , 19 ]; 4 articles on the effect of ethanol on salivary glands [ 6 , 7 , 9 , 20 ]; 9 articles on the effects of heavy metals on salivary glands [ 2 , 11 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ]; 3 articles on the effect of fluoride on salivary gland [ 4 , 10 , 28 ]; 4 articles on the effect of other substances, such as atrazine [ 29 ], high protein diet [ 8 ], hyperoxia [ 30 ], and sodium nitrite [ 31 ]; All included studies were case-control studies. The two types of laboratory animals used were rats and mice.…”

Section: Resultsmentioning

confidence: 99%

“…9 articles on the effects of heavy metals on salivary glands [ 2 , 11 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ];…”

Section: Resultsmentioning

confidence: 99%

“…6 articles on the effect of chemotherapy drugs on salivary glands [5,12,15,16,18,19]; • 4 articles on the effect of ethanol on salivary glands [6,7,9,20]; • 9 articles on the effects of heavy metals on salivary glands [2,11,[21][22][23][24][25][26][27]; • 3 articles on the effect of fluoride on salivary gland [4,10,28]; • 4 articles on the effect of other substances, such as atrazine [29], high protein diet [8], hyperoxia [30], and sodium nitrite [31];…”

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confidence: 99%

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Atrophy and Inflammatory Changes in Salivary Glands Induced by Oxidative Stress after Exposure to Drugs and Other Chemical Substances: A Systematic Review

Ungureanu,

Grădinaru,

Ghiciuc

et al. 2023

Medicina

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Background and Objectives: Oxidative stress is involved in the alterations at the level of salivary glands, being the cause of oral pathologies like xerostomia, periodontitis, gingivitis, leucoplakia, and cancer. It is known that antioxidants can reverse changes induced by drugs or other chemicals in some organs, but the question is whether these substances can reduce or revert the effects of oxidative stress at the salivary gland level. Our aim was to find histopathological data at the level of salivary glands supporting the hypothesis of the reversal of oxidative stress-induced changes after the treatment with substances with antioxidant effect. Materials and Methods: A systematic search was conducted in PubMed, Science Direct, and Springer databases, including research articles on oxidative stress histological aspects and oxidative stress biomarkers induced by drugs or other chemicals on salivary glands. Results: Out of 1756 articles, 25 articles were selected with data on tissue homogenate used for biochemical analysis of oxidative and antioxidative markers, along with routine hematoxylin eosin (HE) and immunohistochemical analysis used for histopathological and immunohistochemical diagnosis. Drugs (antineoplastic drugs, antibiotics, and analgesics), alcohol, heavy metals, and fluoride can cause oxidative stress, resulting in morphological changes in different tissues, including in salivary glands. There are many antioxidants but only a few were evaluated regarding the effects on salivary glands in animal studies, such as hesperidin and selenium, which can reverse the damage induced by cyclophosphamide; 10-dehydrogingerdione (10-DHGD), a compound extracted from ginger, which has a protective effect against the oxidative stress and apoptosis induced by tramadol; and glycyrrhizic acid, which may repair the injuries incurred after the administration of sodium nitrite. Conclusions: Substances such as hesperidin, selenium, 10-dehydrogingerdione, and glycyrrhizic acid are antioxidants with proven restorative effects on salivary glands for the damage induced by oxidative stress after exposure to drugs and other chemical substances; however, demonstrating their similar effects in human salivary glands is challenging.

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“…therefore, caution was warranted before generalization of the relationship between radiation and the risk of BSGNs, and further studies are needed to finally clarify the subject. Furthermore, workplace exposure to aluminum and cadmium is deemed to be a risk factor for salivary gland diseases (Souza‐Monteiro et al, 2022; Zalewska et al, 2004), but unfortunately, there is no corresponding GWAS data. When it is enlarged in the future, a more accurate and comprehensive analysis can be performed.…”

Section: Discussionmentioning

confidence: 99%

Modifiable factors for benign salivary gland neoplasms: A Mendelian randomization study

et al. 2023

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BackgroundObservational studies have found associations between smoking, alcohol, radiation, body mass index (BMI), periodontitis, and the hazard of benign salivary gland neoplasms (BSGNs). Nevertheless, the etiology of BSGNs remains unclear. This study aims to assess the causal association between these modifiable factors and the BSGNs.MethodsGenetic instruments associated with exposures at the genome‐wide significance level were selected from corresponding genome‐wide association studies. The summary statistics for BSGNs were obtained from the FinnGen consortium (2445 cases and 340,054 controls). The inverse variance‐weighted method was used as the primary analysis, and several sensitivity analyses were performed to test the reliability.ResultsGenetically predicted higher lifetime smoking index (odds ratio [OR] = 2.10, p = 0.012) and BMI (OR = 1.58, p = 2.29 × 10−5) were associated with elevated risk of BSGNs, whereas other exposures do not. Sensitivity analyses showed consistency. The causal effect of the lifetime smoking index became more significant after adjusting for BMI (OR = 2.89, p = 0.005) and alcohol consumption (OR = 2.49, p = 0.002). A slight negative association emerged for alcohol consumption with adjustment for cigarettes per day (OR = 0.53, p = 0.034) but disappeared when adjusting for cigarettes per day and BMI.ConclusionThis study supports the independent causal role of lifetime smoking index and BMI in BSGNs risk.

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Salivary Glands after Prolonged Aluminum Exposure: Proteomic Approach Underlying Biochemical and Morphological Impairments in Rats

Cited by 7 publications

References 67 publications

Global Proteomic Profile of Aluminum-Induced Hippocampal Impairments in Rats: Are Low Doses of Aluminum Really Safe?

Global Proteomic Profile of Aluminum-Induced Hippocampal Impairments in Rats: Are Low Doses of Aluminum Really Safe?

Atrophy and Inflammatory Changes in Salivary Glands Induced by Oxidative Stress after Exposure to Drugs and Other Chemical Substances: A Systematic Review

Modifiable factors for benign salivary gland neoplasms: A Mendelian randomization study

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