Salivary gland hyaluronidase in various species of phlebotomine sand flies (Diptera: psychodidae)

Černá, Pavla; Mikeš, Libor; Volf, Petr

doi:10.1016/s0965-1748(02)00109-1

Cited by 41 publications

(46 citation statements)

References 26 publications

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“…Despite the similarity in expression in both species, qualitative and quantitative interspecific variation was present, such as appearance and disappearance of polypeptides, intensity and thickness of protein bands, and the detection of additional polypeptides with molecular weights >100 kDa ( migonei. The differences were in accordance with the published accounts of protein contents of a variety of sand fly species (7,21,38,39). Bloodmeal analysis indicated that these species were opportunistic feeders, with little attraction to a particular host species (32).…”

Section: Resultssupporting

confidence: 88%

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Las glándulas salivales de dos flebotominos vectores de Leishmania: Lutzomyia migonei (França) y Lutzomyia ovallesi (Ortiz) (Diptera: Psychodidae)

et al. 2010

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Introduction. Leishmaniasis is a vector-borne disease transmitted by the intradermal inoculation of Leishmania (Kinetoplastida: Trypanosomatidae) promastigotes together with saliva during the bite of an infected sand fly. Objective. The salivary glands were compared from two vector species, Lutzomyia ovallesi (Ortiz,1952) and Lutzomyia migonei (França,1920) (Diptera: Psychodidae). Material and methods. Protein profiles by SDS PAGE of salivary glands were compared among species as well as their development at several times post feeding. First, mice were immunized to salivary proteins by exposure to biting by L. ovallesi and of L. migonei. Antibodies in these mice against salivary gland-specific proteins were evaluated by immunoblotting. Results. No apparent change was revealed in the kinetic expression of salivary proteins induced by the different physiological states post feeding. Qualitative and quantitative variations were detected in16-18 polypeptides with molecular weights ranging from 6 to 180 kDa. Species-specific proteins were demonstrated for L. migonei and L. ovallesi. In addition, antibodies against salivary gland specific proteins were found in mice immunized by the saliva of both species. Conclusion. Basic information was obtained concerning the nature of salivary gland proteins of L. migonei and L. ovallesi. This information helps to elucidate the role of salivary proteins and their potential as effective tools in screening risk factors in human and other vertebrate hosts.

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Section: Resultssupporting

confidence: 88%

“…Other authors report that after being fed with blood or carbohydrates, the total protein content of sand fly saliva can decrease by half or more of its original quantity (7,39,45,46). These observations provide support for the differences in gland size described above.…”

Section: Resultssupporting

confidence: 54%

Las glándulas salivales de dos flebotominos vectores de Leishmania: Lutzomyia migonei (França) y Lutzomyia ovallesi (Ortiz) (Diptera: Psychodidae)

et al. 2010

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“…S5A. Hyaluronidases are found in many venomous snakes and arthropods (41). They can facilitate the spreading of toxic compounds by degradation of the extracellular matrix (see the review by Kreil (42)).…”

Section: Molecular Mechanism For Successful Blood Feeding In Horseflymentioning

confidence: 99%

Toward an Understanding of the Molecular Mechanism for Successful Blood Feeding by Coupling Proteomics Analysis with Pharmacological Testing of Horsefly Salivary Glands

Yang

et al. 2008

Molecular & Cellular Proteomics

121

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Horseflies are economically important blood-feeding arthropods and also a nuisance for humans and vectors for filariasis. They rely heavily on the pharmacological properties of their saliva to get a blood meal and suppress immune reactions of hosts. Little information is available on antihemostatic substances in horsefly salivary glands; especially no horsefly immune suppressants have been reported. By proteomics or peptidomics and coupling transcriptome analysis with pharmacological testing, several families of proteins or peptides, which act mainly on the hemostatic system or immune system of the host, were identified and characterized from 30,000 pairs salivary glands of the horsefly Tabanus yao (Diptera, Tabanidae). They are: (i) a novel family of inhibitors of platelet aggregation including two members, which possibly inhibit platelet aggregation by a novel mechanism and act on platelet membrane, (ii) a novel family of immunosuppressant peptides including 12 members, which can inhibit interferon-␥ production and increase interleukin-10 secretion, (iii) a serine protease inhibitor with 56 amino acid residues containing anticoagulant activity, (iv) a serine protease with anticoagulant activity, (v) a protease with fibrinogenolytic activity, (vi) three families of antimicrobial peptides including six members, (vii) a hyaluronidase, (viii) a vasodilator peptide, which is an isoform of vasotab identified from Hybomitra bimaculata, and interestingly (ix) two metallothioneins, which are the first metallothioneins reported from invertebrate salivary glands. The current work will facilitate the understanding of the molecular mechanisms of the ectoparasite-host relationship and help in identifying novel vaccine targets and novel leading pharmacological compounds. Molecular & Cellular Proteomics 7:582-590, 2008.

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“…Since the composition of salivary molecules, their function and antigenicity varies among distinct sand fly species [15],[16],[17] it is of foremost importance to investigate whether vector-based vaccines can also be developed against other Leishmania species. American cutaneous leishmaniasis, caused by Leishmania braziliensis , is distinguished from other leishmaniases by its chronicity, latency and tendency to metastasize in the human host leading to muco-cutaneous leishmaniasis [18],[19].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Leishmania braziliensis Infection Following Pre-Exposure to Sandfly Saliva

et al. 2007

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BackgroundSand fly saliva has an array of pharmacological and immunomodulatory components, and immunity to saliva protects against Leishmania infection. In the present study, we have studied the immune response against Lutzomyia intermedia saliva, the main vector of Leishmania braziliensis in Brazil, and the effects of saliva pre-exposure on L. braziliensis infection employing an intradermal experimental model.Methodology/principal findingsBALB/c mice immunized with L. intermedia salivary gland sonicate (SGS) developed a saliva-specific antibody response and a cellular immune response with presence of both IFN-γ and IL-4. The inflammatory infiltrate observed in SGS-immunized mice was comprised of numerous polymorphonuclear and few mononuclear cells. Mice challenged with live L. braziliensis in the presence of saliva were not protected although lesion development was delayed. The inoculation site and draining lymph node showed continuous parasite replication and low IFN-γ to IL-4 ratio, indicating that pre-exposure to L. intermedia saliva leads to modulation of the immune response. Furthermore, in an endemic area of cutaneous leishmaniasis, patients with active lesions displayed higher levels of anti-L. intermedia saliva antibodies when compared to individuals with a positive skin test result for Leishmania.ConclusionThese results show that pre-exposure to sand fly saliva plays an important role in the outcome of cutaneous leishmaniasis, in both mice and humans. They emphasize possible hurdles in the development of vaccines based on sand fly saliva and the need to identify and select the individual salivary candidates instead of using whole salivary mixture that may favor a non-protective response.

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Salivary gland hyaluronidase in various species of phlebotomine sand flies (Diptera: psychodidae)

Cited by 41 publications

References 26 publications

Las glándulas salivales de dos flebotominos vectores de Leishmania: Lutzomyia migonei (França) y Lutzomyia ovallesi (Ortiz) (Diptera: Psychodidae)

Las glándulas salivales de dos flebotominos vectores de Leishmania: Lutzomyia migonei (França) y Lutzomyia ovallesi (Ortiz) (Diptera: Psychodidae)

Toward an Understanding of the Molecular Mechanism for Successful Blood Feeding by Coupling Proteomics Analysis with Pharmacological Testing of Horsefly Salivary Glands

Enhanced Leishmania braziliensis Infection Following Pre-Exposure to Sandfly Saliva

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