Salivary exRNA biomarkers to detect gingivitis and monitor disease regression

Kaczor‐Urbanowicz, Karolina Elżbieta; Trivedi, Harsh M.; Lima, Patrícia Oliveira de; Camargo, Paulo M.; Giannobile, William V.; Grogan, Tristan; Gleber‐Netto, Frederico O.; Whiteman, Yair; Li, Feng; Lee, Hyo‐Jung; Dharia, Karan; Aro, Katri; Carreras‐Presas, Carmen Martín; Amuthan, Saarah; Vartak, Manjiri; Akin, David; Al-adbullah, Hiba; Bembey, Kanika; Klokkevold, Perry R.; Elashoff, David; Barnes, Virginia Monsul; Richter, Rose; DeVizio, William; Masters, James G.; Wong, David T.

doi:10.1111/jcpe.12930

Cited by 17 publications

(18 citation statements)

References 64 publications

(89 reference statements)

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“…Thus, there is now compelling evidence that miRNAs are associated with physiological and pathological process that contribute to the development and progression of systemic diseases, such as cancers [33] and vascular disease [34], as well as periodontal disease [22,35]. Several studies described the potential of miRNAs as putative diagnostic biomarkers for periodontal disease, including hsa-miR-15a-5p, hsa-miR-29b-3p, hsa-miR-124-3p, hsa-miR-140-5p, hsa-miR-146a-5p, hsa-miR-148a-3p, hsa-miR-155-5p, hsa-miR-223-3p, hsa-miR-301b, hsa-miR-628-5p [21][22][23][24][25][26]. It has been reported that hsa-miR-140-5p and hsa-miR-628-5p in gingival crevicular fluid are potential diagnostic biomarkers for periodontitis [25].…”

Section: Discussionmentioning

confidence: 99%

“…Several studies described the potential of miRNAs as putative diagnostic biomarkers for periodontal disease, including hsa-miR-15a-5p, hsa-miR-29b-3p, hsa-miR-124-3p, hsa-miR-140-5p, hsa-miR-146a-5p, hsa-miR-148a-3p, hsa-miR-155-5p, hsa-miR-223-3p, hsa-miR-301b, hsa-miR-628-5p [21][22][23][24][25][26]. It has been reported that hsa-miR-140-5p and hsa-miR-628-5p in gingival crevicular fluid are potential diagnostic biomarkers for periodontitis [25]. Evidence demonstrated hsa-miR-146a-5p might serve as an indicator for periodontal disease severity in both gingival tissues [36] and saliva [37].…”

Section: Discussionmentioning

confidence: 99%

“…However, no studies have explored miRNAs' expression patterns in salivary sEVs as potential biomarkers for periodontal status. According to published reviews [21][22][23][24][25][26], ten periodontitis-associated miRNAs (hsa-miR-15a-5p, hsa-miR-29b-3p, hsa-miR-124-3p, hsa-miR-140-5p, hsa-miR-146a-5p, hsa-miR-148a-3p, hsa-miR-155-5p, hsa-miR-223-3p, hsa-miR-301b, hsa-miR-628-5p) have been explored as potential periodontitis biomarkers from one or more sample sources, such as saliva, gingival tissues, or periodontium-derived cells. However, since miRNAs are expressed in a tissue and biofluid-specific manner, it is important to investigate whether these proposed miRNA periodontitis biomarker candidates are also expressed in salivary sEVs.…”

Section: Introductionmentioning

confidence: 99%

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Salivary Small Extracellular Vesicles Associated miRNAs in Periodontal Status—A Pilot Study

Han

Bartold

Salomón

et al. 2020

IJMS

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This pilot study aims to investigate whether salivary small extracellular vesicle (sEV)-associated microRNAs could act as potential biomarkers for periodontal disease status. Twenty-nine participants (10 who were healthy, nine with gingivitis, 10 with stage III/IV periodontitis) were recruited and unstimulated whole saliva samples were collected. Salivary sEVs were isolated using the size-exclusion chromatography (SEC) method and characterised by morphology, EV-protein and size distribution using transmission electron microscopy (TEM), Western Blot and Nanoparticle Tracking Analysis (NTA), respectively. Ten mature microRNAs (miRNAs) in salivary sEVs and saliva were evaluated using RT-qPCR. The discriminatory power of miRNAs as biomarkers in gingivitis and periodontitis versus healthy controls was evaluated by Receiver Operating Characteristics (ROC) curves. Salivary sEVs were comparable to sEVs morphology, mode, size distribution and particle concentration in healthy, gingivitis and periodontitis patients. Compared to miRNAs in whole saliva, three significantly increased miRNAs (hsa-miR-140-5p, hsa-miR-146a-5p and hsa-miR-628-5p) were only detected in sEVs in periodontitis when compared to that of healthy controls, with a good discriminatory power (area under the curve (AUC) = 0.96) for periodontitis diagnosis. Our study demonstrated that salivary sEVs are a non-invasive source of miRNAs for periodontitis diagnosis. Three miRNAs that are selectively enriched in sEVs, but not whole saliva, could be potential biomarkers for periodontal disease status.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Salivary Small Extracellular Vesicles Associated miRNAs in Periodontal Status—A Pilot Study

Han

Bartold

Salomón

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…[14][15][16][17][18] Many of the diagnostics surveyed fell into this category. PM/PD diagnostics play a signiicant predictive role especially with those measuring predictive biomarkers, which consider speciic risk factors, genetic phenotype, pharmacokinetic characteristics, and other features unique to each patient.…”

Section: Use Of Pm/pd Diagnostics In Clinicsmentioning

confidence: 99%

“…The 2017 survey extended the 2014 survey by investigating the use in DDS/DMD and postgraduate/resident program clinics of seven genetics-based and eight non-genetics-based PM/PD diagnostics kinetic characteristics, and other features unique to each patient. [14][15][16][17][18] Many of the diagnostics surveyed fell into this category. The survey found that Oral DNA Genetics Labs and Periopredict were the two most popular genetics-based PM/PD clinical diagnostic used in these schools, with 5% of participating DDS/DMD programs and 14% of participating postgraduate/ resident programs using OralDNA Labs and 14% of participating postgraduate/resident programs using Periopredict (Figure 2, panel A).…”

Section: Use Of Pm/pd Diagnostics In Clinicsmentioning

confidence: 99%

The Teaching of Personalized Dentistry in North American Dental Schools: Changes from 2014 to 2017

Zheng

Rifkin

Spielman

et al. 2019

Journal of Dental Education

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The aim of this study was to assess the development of personalized dentistry in the curricula of North American dental schools from 2014 to 2017. In 2014, a web‐based survey on personalized medicine/dentistry (PM/PD) was distributed to academic deans of all U.S. (n=65) and Canadian (n=10) dental schools with graduating classes. The results (n=42; 56% response rate) showed that few schools had plans for implementation of PM/PD at the time, even though the majority of respondents reported feeling that PM/PD should be taught in the curriculum and will impact clinical practice in the future. A three‐year followup survey in 2017, sent to the same 75 schools, was designed to reassess the teaching/practice of PM/PD in dental schools in both didactic and clinical curricula. In the results of the 2017 survey (n=30; 40% response rate), the majority of respondents reported feeling that PM/PD should be taught in dental curricula. However, while most respondents indicated their schools did not teach PM/PD as a portion of their didactic curricula, they reported that specific pertinent PM/PD topics were taught as part of other courses in their curricula. The 2017 survey also evaluated the use of seven genetics‐based and eight non‐genetics‐based PM/PD diagnostics in the schools’ clinical curricula. Overall, non‐genetics‐based diagnostics were used more often than genetics‐based diagnostics, and the use of genetics‐based diagnostics was more prevalent in postgraduate than predoctoral clinics. Personalized dentistry will inevitably be part of the dental professional's future and should be reflected in basic science research, clinical settings, and dental school curricula in both predoctoral and postgraduate programs.

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MicroRNAs Function in Dental Stem Cells as a Promising Biomarker and Therapeutic Target for Dental Diseases

Nasiri,

Jahri,

Kolahdouz

et al. 2023

Mol Diagn Ther

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Salivary exRNA biomarkers to detect gingivitis and monitor disease regression

Abstract: The clinical values of the developed salivary exRNA biomarkers are associated with gingivitis regression. They offer strong potential to be advanced for definitive validation and clinical laboratory development test.

Cited by 17 publications

References 64 publications

Salivary Small Extracellular Vesicles Associated miRNAs in Periodontal Status—A Pilot Study

Salivary Small Extracellular Vesicles Associated miRNAs in Periodontal Status—A Pilot Study

The Teaching of Personalized Dentistry in North American Dental Schools: Changes from 2014 to 2017

MicroRNAs Function in Dental Stem Cells as a Promising Biomarker and Therapeutic Target for Dental Diseases

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