Salivary Electrochemical Cortisol Biosensor Based on Tin Disulfide Nanoflakes

Abstract

Cortisol, a steroid hormone, is secreted by the hypothalamic-pituitary-adrenal system. It is a well-known biomarker of psychological stress and is hence known as the “stress hormone.” If cortisol overexpression is prolonged and repeated, dysfunction in the regulation of cortisol eventually occurs. Therefore, a rapid point-of-care assay to detect cortisol is needed. Salivary cortisol electrochemical analysis is a non-invasive method that is potentially useful in enabling rapid measurement of cortisol levels. In this study, multilayer films containing two-dimensional tin disulfide nanoflakes, cortisol antibody (C-M ab ), and bovine serum albumin (BSA) were prepared on glassy carbon electrodes (GCE) as BSA/C-M ab /SnS 2 /GCE, and characterized using electrochemical impedance spectroscopy and cyclic voltammetry. Electrochemical responses of the biosensor as a function of cortisol concentrations were determined using cyclic voltammetry and differential pulse voltammetry. This cortisol biosensor exhibited a detection range from 100 pM to 100 μM, a detection limit of 100 pM, and a sensitivity of 0.0103 mA/Mcm 2 ( R 2 = 0.9979). Finally, cortisol concentrations in authentic saliva samples obtained using the developed electrochemical system correlated well with results obtained using enzyme-linked immunosorbent assays. This biosensor was successfully prepared and used for the electrochemical detection of salivary cortisol over physiological ranges, based on the specificity of antibody-antigen interactions.

3A-Amino-3A-Deoxy-(2AS, 3AS)-β-Cyclodextrin Hydrate/Tin Disulfide Modified Screen-Printed Carbon Electrode for the Electrochemical Detection of Polychlorinated Biphenyls

“…Figure 3 shows the diffraction pattern of SnS 2 exhibited the peaks at 15°, 29°, 30°, 31°, 41°, 46°, 50°, 51°, 53°, and 70°, which are attributed to the (001), (100), (011), (002), (012), (003), (110), (111), (103), and (113) planes, respectively. These results showed the hexagonal phase of SnS 2 [JCPDS (89-2358)], which is confirmation of SnS 2 formation [21].…”

Aptamer-Based Lateral Flow Biosensor for Rapid Detection of Salivary Cortisol

“…at various concentrations provide an opportunity for developing microfluidic POC diagnostics (see the review by Steckl and Ray 4 ). A variety of biomarker detection mechanisms have been employed for this purpose, 4 including colorimetric, 5 electrochemical, 6 , 7 and optical detection. 8 …”

Intelligent Educational Evaluation of Research Performance between Digital Library and Open Government Data

“…This means that there is a new trend in the benchmark technological universities in Taiwan. Biosensors, electrodes, and voltammetry (TC 128) comprise the cow category (high market share and low growth rate) [63,64]. This implies that the topic cluster is in a mature status and is the market leader because of the market share rate of over 1.…”