This study aimed to determine salivary cardiac troponin I and cortisol hormone levels in primary school students to evaluate the cardiac response depending on age and gender variables. The sample of the study consisted of a total of 180 children, including boys and girls aged 8, 9, and 10 years old. The ELISA technique determined cTnI and cortisol hormone levels in saliva samples taken from the children included in the study. There was a significant difference between the salivary cTnI levels and the age levels of the children in the groups (p<0.001). A significant difference was found between the mean level of salivary cortisol hormone and the gender and age variables of the child (p<0.001). As a result of the study, a positive, weak, and statistically significant correlation was found between salivary cTnI and cortisol hormone levels (p<0.05). In the model created for the dependent variable of saliva cTnI, it was found that 7.5% of the variance was explained by the independent variables (R2 adjusted=.075), and the age variable was a statistically significant positive predictor (p<0.001). In the model created for the dependent variable of cortisol hormone, it was found that 48.4% of the variance was explained by the independent variables (R2 adjusted=.484), and age and gender were positive statistically significant predictors (p<0.001). Within the scope of the data obtained in our current study, it was determined that cortisol hormone levels were also high in children with high salivary cardiac troponin I levels. It is thought that examining and monitoring cardiac troponin I and cortisol hormone levels may be useful parameters in the nursing care and practice of children with heart disease risk and disease.