“…Forty newly erupted maxillary lateral incisors were cleaned (Hawe 0270 Latch X30) at slow speed (T1 LINE C 40 L, Sirona, Germany) with non‐fluoridated polishing paste (Cleanic, Kerr Corporation, Orange, CA, USA) for 10 s, gently washed for 40 s, air‐dried and then submitted to a first impression using a polyvinyl siloxane (PVS) impression material (Affinis light body; Coltene, Alstatten, Switzerland), expressed in three layers where the first layer had oil‐free air‐flow applied to the surface to improve the detailed reproduction by the impression material penetration (Baseline) (Gorton and Featherstone, ). Teeth were etched for 15 s with 37% phosphoric acid (Scotchbond, 3M UNITEK, St. Paul, MN, USA), ensuring that the etchant gel was isolated to the test site (Watanabe, ). After etching, the surfaces were rinsed with water from a three‐in‐one syringe for 40 s and another impression was taken using the PVS material (Baseline after etching, T0).…”