Salivary Biomarker Analysis Complementing Regular Clinical Examination

Front, Eran; Laster, Zvi; Unis, Rim; Gavish, Moshe; Nagler, Rafael M.

doi:10.2217/bmm.13.76

Cited by 7 publications

(7 citation statements)

References 32 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results showed that uric acid was significantly higher in thalassemic patients without periodontitis than that in other study groups. This was agree with many studies suggested decreased level of uric acid in saliva of patients with periodontitis like disease (30,31) .…”

Section: Discussionsupporting

confidence: 93%

Oro - Facial Manifestations , Oxidative Stress Marker and Antioxidant in Serum and Saliva of Patients with Beta Thalassemia Major

Shamsah¹,

Zaidan²

2015

JBCD

View full text Add to dashboard Cite

Background: Beta thalassemia is a typically autosomal recessive form of severe anemia which is caused by an imbalance of two types of protein (alpha and beta) subunits of hemoglobin. Oxidative stress imbalance is the equilibrium between pro-oxidant\antioxidant statuses in cellular system, which results in damaging the cells. Antioxidant is a chemical that delays the start or slows the rate of lipid oxidation reaction and it play a very important role in the body defense system against reactive oxygen species. The aims of this study were to recorded the orofacial manifestations in beta thalassemic patients and assess the oxidative stress marker malondialdehyde in serum and salivs and their role in the pathogenesis of beta thalassemia and evaluation the antioxidant uric acid in serum and saliva of those patients. Methods: The study included fifty eight beta thalassemic major patients, twenty eight patients with periodontitis and thirty patients without periodontitis and twenty nine healthy subjects that were age matched with the patients. Orofacial manifestations recorded clinically, serum and saliva malondialdehyde and uric acid were measured in all subjects. Results : The main oro-facial manifestations were malocclusion ,rodent face, brown pigmentation of oral mucosa and incompetent lip.The mean serum and saliva malondialdehyde was significantly higher in thalassemic patients with periodontitis (p<0.001). Serum and saliva uric acid was significantly higher in thalassemic patients without periodontitis (p<0.001). Conclusions: Malocclusion was the most prevalent oro-facial manifestations in beta thalassemic patients, increased serum and saliva malondialdehde refer to the role of oxidative stress in the pathogenesis of beta thalassemia. Uric acid increased to counteract the elevation in the oxidative stress process.

show abstract

Section: Discussionsupporting

confidence: 93%

Oro - Facial Manifestations , Oxidative Stress Marker and Antioxidant in Serum and Saliva of Patients with Beta Thalassemia Major

Shamsah¹,

Zaidan²

2015

JBCD

View full text Add to dashboard Cite

show abstract

“…Potassium transport systems have been associated with pathogenesis in other organisms such as Staphylococcus aureus [ 70 ] and Salmonella [ 71 ], but not in oral bacteria. Interestingly, significant higher levels of potassium have been reported in periodontitis in both gingival crevicular fluid (GCF) and saliva [ 72 , 73 ].…”

Section: Discussionmentioning

confidence: 99%

Functional signatures of oral dysbiosis during periodontitis progression revealed by microbial metatranscriptome analysis

Yost¹,

Duran-Pinedo²,

Teles³

et al. 2015

Genome Med

259

335

View full text Add to dashboard Cite

BackgroundPeriodontitis is a polymicrobial biofilm-induced inflammatory disease that affects 743 million people worldwide. The current model to explain periodontitis progression proposes that changes in the relative abundance of members of the oral microbiome lead to dysbiosis in the host-microbiome crosstalk and then to inflammation and bone loss. Using combined metagenome/metatranscriptome analysis of the subgingival microbiome in progressing and non-progressing sites, we have characterized the distinct molecular signatures of periodontitis progression.MethodsMetatranscriptome analysis was conducted on samples from subgingival biofilms from progressing and stable sites from periodontitis patients. Community-wide expression profiles were obtained using Next Generation Sequencing (Illumina). Sequences were aligned using ‘bowtie2’ against a constructed oral microbiome database. Differential expression analysis was performed using the non-parametric algorithm implemented on the R package ‘NOISeqBio’. We summarized global functional activities of the oral microbial community by set enrichment analysis based on the Gene Ontology (GO) orthology.ResultsGene ontology enrichment analysis showed an over-representation in the baseline of active sites of terms related to cell motility, lipid A and peptidoglycan biosynthesis, and transport of iron, potassium, and amino acids. Periodontal pathogens (Tannerella forsythia and Porphyromonas gingivalis) upregulated different TonB-dependent receptors, peptidases, proteases, aerotolerance genes, iron transport genes, hemolysins, and CRISPR-associated genes. Surprisingly, organisms that have not been usually associated with the disease (Streptococcus oralis, Streptococcus mutans, Streptococcus intermedius, Streptococcus mitis, Veillonella parvula, and Pseudomonas fluorenscens) were highly active transcribing putative virulence factors. We detected patterns of activities associated with progression of clinical traits. Among those we found that the profiles of expression of cobalamin biosynthesis, proteolysis, and potassium transport were associated with the evolution towards disease.ConclusionsWe identified metabolic changes in the microbial community associated with the initial stages of dysbiosis. Regardless of the overall composition of the community, certain metabolic signatures are consistent with disease progression. Our results suggest that the whole community, and not just a handful of oral pathogens, is responsible for an increase in virulence that leads to progression.Trial registrationNCT01489839, 6 December 2011.Electronic supplementary materialThe online version of this article (doi:10.1186/s13073-015-0153-3) contains supplementary material, which is available to authorized users.

show abstract

“…Therefore, in order to solve problems regarding blood and GCF sampling, saliva has been proposed as a source of biomarkers (6,23,24). Previous studies have shown that these biomarkers could be measured in saliva (13,24,25). In this study, LDH salivary level was compared between subjects with periodontitis and subjects without periodontitis.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Whole Salivary Lactate Dehydrogenase Level in Patients With and Without Periodontal Disease

Haghgoo¹,

Soheilifar²,

Bidgoli³

et al. 2016

Avicenna J Dent Res

View full text Add to dashboard Cite

Background: Tracking various biomarkers in serum, gingival crevicular fluid (GCF), and saliva has been introduced as a diagnostic tool for periodontal disease detection. Objectives: The aim of this study was to compare salivary lactate dehydrogenase (LDH) levels in subjects with periodontal disease and levels in subjects without periodontal disease. Materials and Methods:In this case-control study, 170 patients at Hamadan faculty of Dentistry, including patients with periodontal disease and patients with normal periodontium, were selected and divided into test and control groups. Unstimulated saliva was collected in the same situation from the test and control groups. Each saliva sample was analyzed to measure salivary LDH level on the day of collection, by using commercially available kits according to the manufacturers' instructions. A statistical T-test was employed to evaluate significant differences among groups.

show abstract

Salivary Biomarker Analysis Complementing Regular Clinical Examination

Abstract: Salivary composition reflects disease severity and response to therapy, suggesting the efficacy of saliva monitoring for periodontal disease status and care.

Cited by 7 publications

References 32 publications

Oro - Facial Manifestations , Oxidative Stress Marker and Antioxidant in Serum and Saliva of Patients with Beta Thalassemia Major

Oro - Facial Manifestations , Oxidative Stress Marker and Antioxidant in Serum and Saliva of Patients with Beta Thalassemia Major

Functional signatures of oral dysbiosis during periodontitis progression revealed by microbial metatranscriptome analysis

Comparison of Whole Salivary Lactate Dehydrogenase Level in Patients With and Without Periodontal Disease

Contact Info

Product

Resources

About