Salivary and pellicle proteome: A datamining analysis

Schweigel, Hardy; Wicht, Michael J.; Schwendicke, Falk

doi:10.1038/srep38882

Cited by 27 publications

(30 citation statements)

References 53 publications

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“…This emphasizes the potential of this protein to protect against dental erosion. Another protein that was identified in all the conditions was serum albumin, which comes from plasma and it is able to bind ions as calcium [Schweigel et al, 2016]. This can also be important for acid protection, since ovalbumin was shown to reduce the dissolution of hydroxyapatite by acid solutions in vitro [Hemingway et al, 2008;Kosoric et al, 2010].…”

Section: Discussionmentioning

confidence: 99%

Changes in the Proteomic Profile of Acquired Enamel Pellicles as a Function of Their Time of Formation and Hydrochloric Acid Exposure

et al. 2018

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Objective: Changes in the protein profile of acquired enamel pellicles (AEP) formed in vivo over different time periods were evaluated after the application of hydrochloric acid (HCl). Methods: Nine subjects were submitted to dental prophylaxis with pumice. After 3 or 120 min, the teeth were isolated with cotton rolls and 50 μL of 0.1 M HCl (pH 1.0), 0.01 M HCl (pH 2.0), or deionized water were applied on the buccal surface of the teeth for 10 s. The AEP was then collected using an electrode filter paper presoaked in 3% citric acid. After protein extraction, the samples were submitted to reverse-phase liquid chromatography coupled to mass spectrometry (nano LC-ESI-MS/MS). Label-free quantification was performed (Protein Lynx Global Service software). Results: A total of 180 proteins were successfully identified in the AEP samples. The number of identified proteins increased with the time of pellicle formation. Only 4 proteins were present in all the groups (isoforms of IgA, serum albumin, and statherin). The greatest number of proteins identified uniquely in one of the groups was obtained for the groups treated with HCl after 2 h of pellicle formation (approx. 50 proteins). Conclusion: Proteins resistant to removal by HCl, such as serum albumin and statherin, were identified even in the short-term AEP. In addition, 120-min pellicles present many proteins that are resistant to removal by HCl. This suggests an increase in protection against intrinsic acids with the time of pellicle formation, which should be evaluated in future studies.

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Section: Discussionmentioning

confidence: 99%

Changes in the Proteomic Profile of Acquired Enamel Pellicles as a Function of Their Time of Formation and Hydrochloric Acid Exposure

et al. 2018

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“…Although the peri-implant microbiome is unique, these biofilms establish on the surface of implanted materials in a similar manner to that on the natural dentition. A dental pellicle proteome is formed directly on the implant surface [14,15]. Early colonizing microorganisms then attach to the pellicle via van der Waals forces, electrostatic charges, and specific adhesive proteins [16].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Prosthetic Surfaces in the Oral Cavity—A Perspective on Creative Approaches

et al. 2020

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Replacement of missing teeth is an essential component of comprehensive dental care for patients suffering of edentulism. A popular option is implant-supported restorations. However, implant surfaces can become colonized with polymicrobial biofilms containing Candida species that may compromise peri-implant health. To prevent this, implant components may be treated with a variety of coatings to create surfaces that either repel the attachment of viable microorganisms or kill microorganisms on contact. These coatings may consist of nanoparticles of pure elements (more commonly silver, copper, and zinc), sanitizing agents and disinfectants (quaternary ammonium ions and chlorhexidine), antibiotics (cefalotin, vancomycin, and gentamicin), or antimicrobial peptides (AMPs). AMPs in bioactive coatings have a number of advantages. They elicit a protective action against pathogens, inhibit the formation of biofilms, are less toxic to host tissues, and do not prompt inflammatory responses. Furthermore, many of these coatings may involve unique delivery systems to direct their antimicrobial capacity against pathogens, but not commensals. Coatings may also contain multiple antimicrobial substances to widen antimicrobial activity across multiple microbial species. Here, we compiled relevant information about a variety of creative approaches used to generate antimicrobial prosthetic surfaces in the oral cavity with the purpose of facilitating implant integration and peri-implant tissue health.

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“…Human saliva is a biological fluid with enormous diagnostic potential. Because saliva can be noninvasively collected, it provides an attractive alternative for blood, serum or plasma 13 .…”

Section: Introductionmentioning

confidence: 99%

“…In general, most studies view saliva wrongly as a homogeneous body fluid. It is also not stable, but constantly in change, and its composition is affected among other things by sampling methodology, environment, periodicity, oral hygiene, psychological status and general health 6 , 13 , 20 .…”

Section: Introductionmentioning

confidence: 99%

Standardization of a protocol for shotgun proteomic analysis of saliva

et al. 2018

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Saliva contains numerous proteins and peptides, each of them carries a number of biological functions that are very important in maintaining the oral cavity health and also yields information about both local and systemic diseases. Currently, proteomic analysis is the basis for large-scale identification of these proteins and discovery of new biomarkers for distinct diseases. Objective This study compared methodologies to extract salivary proteins for proteomic analysis.Material and Methods Saliva samples were collected from 10 healthy volunteers. In the first test, the necessity for using an albumin and IgG depletion column was evaluated, employing pooled samples from the 10 volunteers. In the second test, the analysis of the pooled samples was compared with individual analysis of one sample. Salivary proteins were extracted and processed for analysis by LC-ESI-MS/MS.Results In the first test, we identified only 35 proteins using the albumin and IgG depletion column, while we identified 248 proteins without using the column. In the second test, the pooled sample identified 212 proteins, such as carbonic anhydrase 6, cystatin isoforms, histatins 1 and 3, lysozyme C, mucin 7, protein S100A8 and S100A9, and statherin, while individual analysis identified 239 proteins, among which are carbonic anhydrase 6, cystatin isoforms, histatin 1 and 3, lactotransferrin, lyzozyme C, mucin 7, protein S100A8 and S100A9, serotransferrin, and statherin.Conclusions The standardization of protocol for salivary proteomic analysis was satisfactory, since the identification detected typical salivary proteins, among others. The results indicate that using the column for depletion of albumin and IgG is not necessary and that performing individual analysis of saliva samples is possible.

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Salivary and pellicle proteome: A datamining analysis

Cited by 27 publications

References 53 publications

Changes in the Proteomic Profile of Acquired Enamel Pellicles as a Function of Their Time of Formation and Hydrochloric Acid Exposure

Changes in the Proteomic Profile of Acquired Enamel Pellicles as a Function of Their Time of Formation and Hydrochloric Acid Exposure

Antimicrobial Prosthetic Surfaces in the Oral Cavity—A Perspective on Creative Approaches

Standardization of a protocol for shotgun proteomic analysis of saliva

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