Salivacin 140, a novel bacteriocin from Lactobacillus salivarius subsp. salicinius T140 active against pathogenic bacteria

The aims of the present study were to screen and characterize the antimicrobial lactic acid bacteria which were isolated from healthy oral cavities of Thai volunteers, and to characterize their inhibiting substances. Among 3790 isolates (suspected to be lactic acid bacteria) from 130 volunteers, ®ve showed an appreciable effect against Sarcina lutea ATCC 9341, Bacillus cereus ATCC 11778, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538, Streptococcus mutans DTMU 1, Strep. salivarius DTMU 1, Strep. sanguis DTMU 1, Candida albicans ATCC 13803 and C. albicans DTMU 2, as well as the oral pathogens. These antimicrobial isolates included L17 and N14 which showed the antibacterial activity, D14 which showed the anticandidal activity, and D6 and N8 which showed both the antibacterial and anticandidal activities. The isolates were later found to be facultative anaerobic, Gram-positive, non-spore-forming, non-capsuleforming and catalase-negative bacilli. They could utilize casein but could not hydrolyse starch, and they produced hydrogen peroxide and bacteriocins. Their antimicrobial potentials were found to be affected by pH, catalase, proteolytic enzymes and temperature. The activity was partially inactivated after catalase treatment, signi®cantly declined at pH ³9á0 or after trypsin and pepsin treatments, and also reduced after heating at ³100°C. However, the antimicrobial activity of these ®ve isolates was somewhat resistant to heat. When the isolates were tested for their antimicrobial sensitivity, they were shown to be sensitive to a number of antimicrobial agents. The ®nal identi®cation revealed that D6, D14 and N14 were Lactobacillus paracasei subsp. paracasei, and L17 and N8 were Lact. rhamnosus.

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“…1998). Some LAB produce bacteriocins which are proteinaceous substances with antimicrobial activity (Arihara et al . 1996; van Reenen et al .…”

Section: Discussionmentioning

confidence: 99%

Lactic acid bacteria from healthy oral cavity of Thai volunteers: inhibition of oral pathogens

2001

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“…The production of bacteriocin or bacteriocin-like substances has been shown in some strains of L. salivarius samples taken from grass leaves [Arihara et al, 1996], human vaginas [Juarez Tomas et al, 2002] as well as in animals [Robredo and Torres, 2000] and these were found to have inhibitory or bactericidal actions against various bacteria, including S. mutans [Arihara et al, 1996]. In the present study, superinfection with L. salivarius LS1952R did not reduce or inhibit the establishment of S. mutans MT8148R in the oral cavity of rats.…”

Section: Discussionmentioning

confidence: 99%

Cariogenicity of the Probiotic Bacterium <i>Lactobacillus salivarius</i> in Rats

et al. 2005

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Probiotic bacteria such as lactobacilli and bifidobacteria are considered to be non-pathogenic and non-toxigenic on the basis of long years of safe usage. However, some species of lactobacilli are thought to be associated with the development of dental caries. The purpose of the present study was to examine the cariogenicity of the probiotic bacterium Lactobacillus salivarius in rats. Rats were divided into six groups, and infected with L. salivarius LS1952R and/or Streptococcus mutans MT8148R. L. salivarius LS1952R became established in the oral cavity of rats and induced significant level of dental caries even when infected for only 5 days from 18 to 22 days of age. In addition, the caries scores of rats superinfected with both Streptococcus mutans MT8148R and L. salivarius LS1952R from 18 days of age were significantly higher than those infected with either L. salivarius LS1952R or S. mutans MT8148R alone. Since strain LS1952R can adhere to saliva-coated hydroxyapatites, it is concluded that L. salivarius strain LS1952R possesses an inherent cariogenic activity following adherence to the tooth surface.

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“…Indeed, intensive research over the last 20 years has identified several novel bacteriocins, many of which are produced by intestinal and/or probiotic bacteria (13). The production of bacteriocins by Lactobacillus salivarius strains has been reported previously (1,12,14,17), and ABP-118, a twocomponent class II bacteriocin produced by a human intestinal probiotic strain, L. salivarius subsp. salivarius UCC118, has been fully characterized at the molecular level (6).…”

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confidence: 99%

Salivaricin P, One of a Family of Two-Component Antilisterial Bacteriocins Produced by Intestinal Isolates of Lactobacillus salivarius

Barrett

Hayes

O’Connor

et al. 2007

Appl Environ Microbiol

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Lactobacillus salivarius DPC6005, a porcine intestinal isolate, produces a two-component bacteriocin, salivaricin P, with homology to ABP-118 produced by a human probiotic L. salivarius strain. Indeed, molecular characterization revealed that while the peptides Sln1 and ABP-118␣ are identical, their companion peptides (Sln2 and ABP-118␤, respectively) differ by two amino acids. This observation suggests that two-component bacteriocins may be a common feature of intestinal L. salivarius strains.A large number of lactic acid bacteria produce bacteriocins, which are ribosomally synthesized and secreted peptides which kill other microorganisms. Indeed, intensive research over the last 20 years has identified several novel bacteriocins, many of which are produced by intestinal and/or probiotic bacteria (13). The production of bacteriocins by Lactobacillus salivarius strains has been reported previously (1,12,14,17), and ABP-118, a twocomponent class II bacteriocin produced by a human intestinal probiotic strain, L. salivarius subsp. salivarius UCC118, has been fully characterized at the molecular level (6). Indeed, recent genome sequencing of the producing strain revealed that this bacteriocin is encoded on a 242-kb megaplasmid (4). In this paper, we describe the characterization of a two-component bacteriocin which is remarkably similar to ABP-118 and is produced by a porcine intestinal isolate, L. salivarius DPC6005, which was included in a five-strain probiotic mixture which contributed to reducing the numbers of Enterobacteriaceae by up to 98% in a porcine model without affecting the total fecal Lactobacillus population (7). Furthermore, we describe detection of a number of other intestinal strains with the ability to produce salivaricin P, a finding which suggests that the production of two-component antilisterial bacteriocins is prevalent among intestinal members of L. salivarius.All bacterial strains used in this study are shown in Table 1, and all strains were stored at Ϫ80°C in 40% (vol/vol) glycerol. L. salivarius subsp. salivarius UCC118 was isolated previously from the human gastrointestinal tract and was obtained from University College, Cork, Ireland, under a restricted-material transfer agreement. Lactobacilli were grown anaerobically at 37°C in MRS (Oxoid Ltd., Hampshire, England). Anaerobic conditions were maintained in Anaerocult anaerobic jars (Merck, Darmstadt, Germany), using Anaerocult A gas packs (Merck). Lactococcal strains were propagated at 30°C in M17 (Difco Laboratories, Detroit, MI) supplemented with 0.5% (wt/ vol) lactose and grown aerobically. Listeria innocua DPC3572 and enterococcal strains were propagated at 37°C in M17 supplemented with 0.5% (wt/vol) glucose and grown aerobically. Pediococci and Bacillus subtilis were propagated at 30°C in M17 supplemented with 0.5% (wt/vol) glucose and grown aerobically. Leuconostoc sp. was grown anaerobically at 30°C in MRS. Solid agar media were prepared by addition of 1% (wt/vol) agar (Difco) to the broth media. The bacteriocin-producing capabil...

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Salivacin 140, a novel bacteriocin from Lactobacillus salivarius subsp. salicinius T140 active against pathogenic bacteria

Cited by 31 publications

References 17 publications

Lactic acid bacteria from healthy oral cavity of Thai volunteers: inhibition of oral pathogens

Lactic acid bacteria from healthy oral cavity of Thai volunteers: inhibition of oral pathogens

Cariogenicity of the Probiotic Bacterium <i>Lactobacillus salivarius</i> in Rats

Salivaricin P, One of a Family of Two-Component Antilisterial Bacteriocins Produced by Intestinal Isolates of Lactobacillus salivarius

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