Salinomycin encapsulated nanoparticles as a targeting vehicle for glioblastoma cells

Aydın, R. Seda Tığlı; Kaynak, Gökçe; Gümüşderelı́oğlu, Menemşe

doi:10.1002/jbm.a.35591

Cited by 24 publications

(9 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Salinomycin was 100-fold more effective towards CSCs than the conventional chemotherapeutic drug paclitaxel and decreased the percentage of CD44 high /CD24 low breast CSCs by 20-fold [ 124 ]. Hyaluronic acid-coated salinomycin nanoparticles decreased the expression of CD44 in breast CSCs and polysorbate 80-coated poly (lactic-co-glycolic acid)-encapsulated salinomycin nanoparticles enhanced cell death in glioblastoma [ 125 , 126 ]. The synergistic action of rGO–Ag and salinomycin induced apoptosis through caspase-dependent and caspase-independent pathways and was involved in the loss of membrane potential of mitochondria.…”

Section: Resultsmentioning

confidence: 99%

Graphene Oxide–Silver Nanocomposite Enhances Cytotoxic and Apoptotic Potential of Salinomycin in Human Ovarian Cancer Stem Cells (OvCSCs): A Novel Approach for Cancer Therapy

Choi

Gurunathan

Kim

2018

IJMS

View full text Add to dashboard Cite

The use of graphene to target and eliminate cancer stem cells (CSCs) is an alternative approach to conventional chemotherapy. We show the biomolecule-mediated synthesis of reduced graphene oxide–silver nanoparticle nanocomposites (rGO–Ag) using R-phycoerythrin (RPE); the resulting RPE–rGO–Ag was evaluated in human ovarian cancer cells and ovarian cancer stem cells (OvCSCs). The synthesized RPE–rGO–Ag nanocomposite (referred to as rGO–Ag) was characterized using various analytical techniques. rGO–Ag showed significant toxicity towards both ovarian cancer cells and OvCSCs. After 3 weeks of incubating OvCSCs with rGO–Ag, the number of A2780 and ALDH+CD133+ colonies was significantly reduced. rGO–Ag was toxic to OvCSCs and reduced cell viability by mediating the generation of reactive oxygen species, leakage of lactate dehydrogenase, reduced mitochondrial membrane potential, and enhanced expression of apoptotic genes, leading to mitochondrial dysfunction and possibly triggering apoptosis. rGO–Ag showed significant cytotoxic potential towards highly tumorigenic ALDH+CD133+ cells. The combination of rGO–Ag and salinomycin induced 5-fold higher levels of apoptosis than each treatment alone. A combination of rGO–Ag and salinomycin at very low concentrations may be suitable for selectively killing OvCSCs and sensitizing tumor cells. rGO–Ag may be a novel nano-therapeutic molecule for specific targeting of highly tumorigenic ALDH+CD133+ cells and eliminating CSCs. This study highlights the potential for targeted therapy of tumor-initiating cells.

show abstract

Section: Resultsmentioning

confidence: 99%

Graphene Oxide–Silver Nanocomposite Enhances Cytotoxic and Apoptotic Potential of Salinomycin in Human Ovarian Cancer Stem Cells (OvCSCs): A Novel Approach for Cancer Therapy

Choi

Gurunathan

Kim

2018

IJMS

View full text Add to dashboard Cite

show abstract

“…These results show that GBM cells (T98G and LN18) expressing higher levels of all four genes are less sensitive to celastrol and triptolide treatments and also highly resistant to TMZ treatment. However, intriguingly in terms of salinomycin-sensitivity [ [50] , [51] , [52] , [53] , [54] ], T98G and LN229 cells are more sensitive than LN18 and U87 cells accessed by both cell proliferation (the EC50 for T98G and LN18 is approximately 1.25 μM, while the EC50 for LN229 and U87 is about 10 times higher than T98G and LN18) and tumor-sphere formation assays ( Fig. 7 d, g).…”

Section: Resultsmentioning

confidence: 99%

Identification of a panel of genes as a prognostic biomarker for glioblastoma

et al. 2018

View full text Add to dashboard Cite

BackgroundGlioblastoma multiforme (GBM) is a fatal disease without effective therapy. Identification of new biomarkers for prognosis would enable more rational selections of strategies to cure patients with GBM and prevent disease relapse.MethodsSeven datasets derived from GBM patients using microarray or next generation sequencing in R2 online database (http://r2.amc.nl) were extracted and then analyzed using JMP software. The survival distribution was calculated according to the Kaplan-Meier method and the significance was determined using log-rank statistics. The sensitivity of a panel of GBM cell lines in response to temozolomide (TMZ), salinomycin, celastrol, and triptolide treatments was evaluated using MTS and tumor-sphere formation assay.FindingsWe identified that CD44, ATP binding cassette subfamily C member 3 (ABCC3), and tumor necrosis factor receptor subfamily member 1A (TNFRSF1A) as highly expressed genes in GBMs are associated with patients' poor outcomes and therapy resistance. Furthermore, these three markers combined with MGMT, a conventional GBM marker, can classify GBM patients into five new subtypes with different overall survival time in response to treatment. The four-gene signature and the therapy response of GBMs to a panel of therapeutic compounds were confirmed in a panel of GBM cell lines.InterpretationThe data indicate that the four-gene panel can be used as a therapy response index for GBM patients and potential therapeutic targets. These results provide important new insights into the early diagnosis and the prognosis for GBM patients and introduce potential targets for GBM therapeutics.FundBaylor Scott & White Health Startup Fund (E.W.); Collaborative Faculty Research Investment Program (CFRIP) of Baylor University, Baylor Scott & White Health, and Baylor College of Medicine (E.W., T.S., J.H.H.); NIH R01 NS067435 (J.H.H.); Scott & White Plummer Foundation Grant (J.H.H.); National Natural Science Foundation of China 816280007 (J.H.H. and Fu.W.).

show abstract

“…Salinomycin is an antibacterial and ionophore anticoccidial therapeutic drug, which has recently been introduced as a novel alternative to traditional anti-cancer drugs 19 , 20 . In 2009, Gupta et al .…”

Section: Introductionmentioning

confidence: 99%

Salinomycin-loaded Nanofibers for Glioblastoma Therapy

Norouzi

Abdali

Liu

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Salinomycin is an antibiotic that has recently been introduced as a novel and effective anti-cancer drug. In this study, PLGA nanofibers (NFs) containing salinomycin (Sali) were fabricated by electrospinning for the first time. The biodegradable PLGA NFs had stability for approximately 30 days and exhibited a sustained release of the drug for at least a 2-week period. Cytotoxicity of the NFs + Sali was evaluated on human glioblastoma U-251 cells and more than 50% of the treated cells showed apoptosis in 48 h. Moreover, NFs + Sali was effective to induce intracellular reactive oxygen species (ROS) leading to cell apoptosis. Gene expression studies also revealed the capability of the NFs + Sali to upregulate tumor suppressor Rbl1 and Rbl2 as well as Caspase 3 while decreasing Wnt signaling pathway. In general, the results indicated anti-tumor activity of the Sali-loaded NFs suggesting their potential applications as implantable drug delivery systems in the brain upon surgical resection of the tumor.

show abstract

Salinomycin encapsulated nanoparticles as a targeting vehicle for glioblastoma cells

Cited by 24 publications

References 42 publications

Graphene Oxide–Silver Nanocomposite Enhances Cytotoxic and Apoptotic Potential of Salinomycin in Human Ovarian Cancer Stem Cells (OvCSCs): A Novel Approach for Cancer Therapy

Graphene Oxide–Silver Nanocomposite Enhances Cytotoxic and Apoptotic Potential of Salinomycin in Human Ovarian Cancer Stem Cells (OvCSCs): A Novel Approach for Cancer Therapy

Identification of a panel of genes as a prognostic biomarker for glioblastoma

Salinomycin-loaded Nanofibers for Glioblastoma Therapy

Contact Info

Product

Resources

About