Salinity Influence upon Activity of Trichoderma harzianum against Botrytis cinerea

Abstract

Three isolates of Botrytis cinerea were isolated from leaves and stems of different tomato varieties taken from four areas in the Northwest of Algeria where tomato is mostly grown in greenhouses and high tunnels. The purpose of this study was to determine the effect of two salts: NaCl and CaCl 2 tolerance of Trichoderma harzianum and to evaluate the impact of salinity on its antagonistic capacities in order to use it as a biological agent controlling Botrytis cinerea, the causal agent of grey mold disease of tomato. In the absence of salt, the volatile and non-volatile secondary metabolites from T. harzianum showed 52, 23-79, 81% inhibition of B. cinerea. In the presence of salt, the inhibition percentages of the mycelial growth of B. cinerea by volatile metabolites were stimulated by the high concentrations of NaCl 94,70 and 90,85% for CaCl 2 compared to the control. However, non-volatile compounds from Trichoderma, the percentage of growth inhibition varied between 65.17% and 82, 12% for NaCl and 61.19-85.01 in the presence of CaCl 2 .

Combined comparative genomics and molecular biology approaches provide insights into the terpene synthases inventory in Trichoderma

“…Nitrogen availability has a considerable impact on secondary metabolism in fungi (Hautbergue et al, 2018); in Fusarium fujikuroi , it affected the expression of 3 out of 10 TS genes (Wiemann et al, 2013). Similarly, saline stress modulates changes in SMs production, as it has been shown in T. harzianum (Bualem, Mohamed, & Moulay, 2015). N starvation and saline stresses negatively regulated TS genes inT.…”

Combined Comparative Genomics and Gene Expression Analyses Provide Insights into the Terpene Synthases Inventory in Trichoderma

“…Nitrogen availability and saline stress have a considerable impact on fungal secondary metabolism [ 117 , 118 , 119 ]. N starvation and saline stresses negatively regulated TS genes in T. gamsii T6085, suggesting terpenoid biosynthesis does not confer particular advantages to the fungus to overcome these stresses.…”