Salinity-induced cellular cross-talk in carbon partitioning reveals starch-to-lipid biosynthesis switching in low-starch freshwater algae

Zhang, Lijie; Pei, Haiyan; Chen, Shuaiqi; Jiang, Liqun; Hou, Qingjie; Yang, Zhiyong; Yu, Ze

doi:10.1016/j.biortech.2017.11.067

Cited by 103 publications

(41 citation statements)

References 46 publications

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“…1d). The saltcaused growth impairment to different degrees has been reported for many other freshwater algal strains [25][26][27][28][29][31][32][33][34][35]. The content of total fatty acids (TFA) showed a considerable increase (over twofold) under the moderate salt concentration of 0.2 M, but did not increase further under high salt concentrations ( Fig.…”

Section: Optimization Of Salinity Levels For Lipid and Astaxanthin Prmentioning

confidence: 56%

“…Seemingly, starch serves as a temporary carbon sink and is transformed to TAG for long storage, which has been observed in several algae under ND conditions [15,89,100]. It has also been reported that salt stress triggered TAG accumulation at the expense of starch in algae [26,35,39,101], but the underlying mechanism remains rarely touched. We found in C. zofingiensis that coordinated up-regulation of multiple biological pathways contributed to the transition of starch to TAG upon salt stress, which are discussed as following.…”

Section: The Salt Stress-associated Diversion Of Carbon From Starch Tmentioning

confidence: 99%

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Novel insights into salinity-induced lipogenesis and carotenogenesis in the oleaginous astaxanthin-producing alga Chromochloris zofingiensis: a multi-omics study

Mao

Zhang

Wang

et al. 2020

Biotechnol Biofuels

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Background: Chromochloris zofingiensis, a freshwater alga capable of synthesizing both triacylglycerol (TAG) and astaxanthin, has been receiving increasing attention as a leading candidate producer. While the mechanism of oleaginousness and/or carotenogenesis has been studied under such induction conditions as nitrogen deprivation, high light and glucose feeding, it remains to be elucidated in response to salt stress, a condition critical for reducing freshwater footprint during algal production processes. Results:Firstly, the effect of salt concentrations on growth, lipids and carotenoids was examined for C. zofingiensis, and 0.2 M NaCl demonstrated to be the optimal salt concentration for maximizing both TAG and astaxanthin production. Then, the time-resolved lipid and carotenoid profiles and comparative transcriptomes and metabolomes were generated in response to the optimized salt concentration for congruent analysis. A global response was triggered in C. zofingiensis allowing acclimation to salt stress, including photosynthesis impairment, ROS build-up, protein turnover, starch degradation, and TAG and astaxanthin accumulation. The lipid metabolism involved a set of stimulated biological pathways that contributed to carbon precursors, energy and reductant molecules, pushing and pulling power, and storage sink for TAG accumulation. On the other hand, salt stress suppressed lutein biosynthesis, stimulated astaxanthin biosynthesis (mainly via ketolation), yet had little effect on total carotenoid flux, leading to astaxanthin accumulation at the expense of lutein. Astaxanthin was predominantly esterified and accumulated in a well-coordinated manner with TAG, pointing to the presence of common regulators and potential communication for the two compounds. Furthermore, the comparison between salt stress and nitrogen deprivation conditions revealed distinctions in TAG and astaxanthin biosynthesis as well as critical genes with engineering potential. Conclusions:Our multi-omics data and integrated analysis shed light on the salt acclimation of C. zofingiensis and underlying mechanisms of TAG and astaxanthin biosynthesis, provide engineering implications into future trait improvements, and will benefit the development of this alga for production uses under saline environment, thus reducing the footprint of freshwater.

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Section: Optimization Of Salinity Levels For Lipid and Astaxanthin Prmentioning

confidence: 56%

Section: The Salt Stress-associated Diversion Of Carbon From Starch Tmentioning

confidence: 99%

Novel insights into salinity-induced lipogenesis and carotenogenesis in the oleaginous astaxanthin-producing alga Chromochloris zofingiensis: a multi-omics study

Mao

Zhang

Wang

et al. 2020

Biotechnol Biofuels

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“…In a previous study it has been shown that salinity stress increases neutral lipid content of algal cells (Ji et al, 2018). In the fresh water microalga Chlorella sorokiniana grown on BG11 salt stress produced a carbon redistribution from starch and protein to lipid (Kim et al, 2016;Zhang et al, 2018). However in another study dealing with salinity stress in Acutodesmus dimorphus grown on BG11 the carbon redistribution was from protein to lipid and carbohydrate (Chokshi et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Lipids extracted from algal biomass, particularly TAG (Triacylglycerol), are often composed of C 16 and C 18 series of long chain fatty acids, similar to those of vegetable oils suitable for biodiesel production (Ratledge and Wynn, 2002;Miao and Wu, 2006). Fresh water microalgae grown on BG11 under NaCl stress could change their FAMEs profile with a decrease in the saturated fatty acid content (Zhang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Stressing Algae for Biofuel Production: Biomass and Biochemical Composition of Scenedesmus dimorphus and Selenastrum minutum Grown in Municipal Untreated Wastewater

2018

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Biofuel production using microalgae is a renewable and environmental-friendly alternative to the use of fossil fuels. Microalgae storage lipids are promising resources for biofuel production. In this study, pure strains of the microalgae Scenedesmus dimorphus and Selenastrum minutum were grown in untreated municipal wastewater for six days under mixotrophic conditions. The algae strains were subjected to different stresses such as nutrient deprivation, and 5% (w/v) salinity to trigger lipid production and to study effect on FAME composition. The highest lipid concentrations were found in S. dimorphus (35 and 34%) and in S. minutum (40 and 39%) under nutrient deprivation and 5% salinity, respectively. On the one hand, salt stress decreased biomass production; on the other hand in both S. dimorphus and S. minutum salt stress significantly increased the concentration of saturated fatty acid (SFA) and it decreased the concentration of poly-unsaturated fatty acid (PUFA) contents, which are desirable for the production of good quality biofuel such as biodiesel. Hence our findings show how salt stress could clearly affect FAME composition in short time 1-3 days, greatly improving the FAME quality as source of biofuel.

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“…Isso demonstra que, provavelmente, os fotoassimilados foram realocados para o crescimento das raízes, pois ao retirar toda a sua raiz, a planta provavelmente direcionou uma grande parte de sua energia para essa aérea afetada, a fim de retomar o crescimento nessas estruturas, diminuindo, assim, o crescimento da parte aérea (MASHAMAITE; DUBE; PHIRI, 2019;KANG et al, 2019). Vários autores indicam que algumas situações às quais o vegetal é exposto podem proporcionar mudança nos padrões de fixação, alocação e particionamento de biomassa (BREUER et al, 2015;FERNANDES et al, 2016;LIU et al, 2018;PESCHIUTTA et al, 2018;PILON et al, 2018;ZHANG et al, 2019;ZHANG, L. et al, 2018;.…”

Section: Resultado E Discussãounclassified

Incrementos Na Produção De Biomassa Total De Citronela Por Estresse Severo No Sistema Radicular

Daflon

Hüther

Santos

et al. 2019

Rev. Bras. Ciênc. Ambient. (Online)

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Para se obter a melhor qualidade terapêutica e produtividade das plantasmedicinais, o manejo adequado da cultura é fundamental, pois diversosfatores podem interferir. Assim, analisou-se o crescimento das plantas decitronela submetidas a diferentes níveis de cortes na parte aérea e nas raízes.Cinco diferentes proporções de desbaste na raiz (0, 25, 50, 75 e 100%), após145 dias do plantio das mudas e 4 cortes na parte aérea (folhas): sem corte; umcorte aos 145 dias após o plantio (DAP), juntamente com o corte da raiz; um corteaos 228 DAP; e cortes aos 145 e 228 DAP (dois cortes). Foram realizadas quatrocoletas para dados de acúmulo de matéria seca e partição de fotoassimilados.O tratamento com 100% de corte na raiz, mas sem corte nas folhas, aumentouo acúmulo de massa seca em relação aos demais tratamentos, para o últimoperíodo de análise, demonstrando uma recuperação.

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Salinity-induced cellular cross-talk in carbon partitioning reveals starch-to-lipid biosynthesis switching in low-starch freshwater algae

Cited by 103 publications

References 46 publications

Novel insights into salinity-induced lipogenesis and carotenogenesis in the oleaginous astaxanthin-producing alga Chromochloris zofingiensis: a multi-omics study

Novel insights into salinity-induced lipogenesis and carotenogenesis in the oleaginous astaxanthin-producing alga Chromochloris zofingiensis: a multi-omics study

Stressing Algae for Biofuel Production: Biomass and Biochemical Composition of Scenedesmus dimorphus and Selenastrum minutum Grown in Municipal Untreated Wastewater

Incrementos Na Produção De Biomassa Total De Citronela Por Estresse Severo No Sistema Radicular

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