Salinisphaera orenii sp. nov., isolated from a solar saltern

Abstract

A taxonomic study was performed on two isolates, designated strains MK-B5T and MK-B7, isolated from sediment of a solar saltern pond in Gomso Bay, Republic of Korea. Comparative 16S rRNA gene sequence analysis showed that strains MK-B5T and MK-B7 belong to the Gammaproteobacteria and are related most closely to Salinisphaera shabanensis JCM 11575T ( = E1L3AT) (96.3 and 96.5 % similarity, respectively), Salinisphaera dokdonensis KCCM 90064T ( = CL-ES53T) (95.6 and 95.6 %) and Salinisphaera hydrothermalis JCM 115514T ( = EPR70T) (95.1 and 95.3 %). The level of 16S rRNA gene sequence similarity between strains MK-B5T and MK-B7 was 99.8 %. The G+C contents of their genomic DNAs were 63.4 and 63.6 mol%, respectively, and the major respiratory quinone was ubiquinone-8. DNA–DNA relatedness between strains MK-B5T and MK-B7 was 98 %, indicating that the two isolates represent a single species. However, the level of DNA–DNA relatedness between the two isolates and S. shabanensis E1L3AT (26.4–30.8 %) indicates that they represent a novel species. Strains MK-B5T and MK-B7 possessed C14 : 0, C16 : 0 and C19 : 0ω8c cyclo as major fatty acids. The two isolates were Gram-stain-negative, strictly aerobic, short rod-shaped and motile. They grew at 10–40 °C (optimum, 35–37 °C), at pH 5.0–8.5 (optimum, 7.0–7.5) and with 5–25 % (w/v) NaCl (optimum, 15 % NaCl). On the basis of phenotypic and phylogenetic analyses, strains MK-B5T and MK-B7 are thus considered to represent a novel species of the genus Salinisphaera , for which the name Salinisphaera orenii sp. nov. is proposed. The type strain is MK-B5T ( = KCTC 23198T = JCM 17073T).

The Saltpan Microbiome Is Structured by Sediment Depth and Minimally Influenced by Variable Hydration

“…Many of the prominent bacterial taxa that were observed in saltpan sediments are common seawater and coastal marine microorganisms. Generally, the surface sediment was dominated by common marine taxa [46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62] and several others which have been previously isolated from tidal flats [63][64][65], salt evaporates [66], and other saline environments [67][68][69]. Based on relative abundance, many of these taxa are likely transient and occur with flood or storm tides.…”

“…They would only become less abundant relative to marine taxa due to random flood immigration events. Finally, such halophiles as Salinisphaera, Marivita, Alteromonas, Marinobacter, halobacteriaceae, and Haloferacaceae [66][67][68][69]80,81] were restricted to the surface alone and their relative abundance may be due to either effect, likely receiving contributions from both deterministic and random processes.…”

Halolamina sediminis sp. nov., an extremely halophilic archaeon isolated from solar salt

“…Strain halo-7 T was isolated from a sediment sample collected from a crystallizing pond of GomSo solar saltern (salinity 17–23 %, temperature 35 °C and pH 7.8; 35° 35′ N 126° 36′ E, data from Park et al , 2012). A 2 g brine sediment sample was collected in a sterile conical tube and serially diluted in filter-sterilized (0.22 μm pore size, Millipore) natural brine water with 0.5 % (w/v) yeast extract (Difco).…”

Halobellus ramosii sp. nov., an extremely halophilic archaeon isolated from a saline-wetland wildfowl reserve

“…The strain was cultured in MH medium containing the following concentrations of a balanced mixture of sea salts (Subov, 1931): 3, 5, 7.5, 10, 12.5, 15, 17.5, 20, 22.5, 25 and 30 % (w/v). Tolerance to pH was tested in MH broth media at pH 4–10 (at intervals of 0.5) adjusted with three different buffers solutions (final concentration, 10 mM): acetate buffer, pH 4–5.5; phosphate buffer, pH 6.0–8.0; Tris buffer, pH 8.5–10 (Park et al , 2012). The requirement for magnesium was tested in media containing final concentrations of 0.0, 0.005, 0.1, 0.2, 0.4, 0.8, 1.6 and 3.2 % (w/v) MgCl 2 at the optimum NaCl concentration for growth.…”