Salidroside ameliorates liver metabonomics in relation to modified gut-liver FXR signaling in furan-induced mice

Yuan, Yuan; Wu, Xuan; Yuan, Hong; Zhang, Xu; Wang, Ziyue; Yan, Haiyang

doi:10.1016/j.fct.2020.111311

Cited by 14 publications

(8 citation statements)

References 40 publications

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“…Furan leads to hepatocyte apoptosis, 12 while SAL reduces apoptosis. 30 Our previous research has demonstrated the ameliorative effects of SAL against furan-induced liver damage, 19,26,27 and we assumed that the effects involved antiapoptosis due to the close relationship between hepatocyte apoptosis and liver injury. We therefore focused on the ameliorating mechanism of SAL against furan-induced apoptosis of hepatocytes, and the protective effects were investigated by serum metabolomics.…”

Section: Discussionmentioning

confidence: 94%

“…In accordance with the methods detailed in our earlier research, serum samples were prepared and LC-MS/MS analyses were performed. 26 Briefly, 50% methanol buffer was used to extract the metabolites in mouse serum samples, and the mixture of each extract was applied to formulate pooled quality control (QC) samples. The LC-MS/MS analysis was carried out by LC-Bio Technology Co., Ltd (Hang Zhou, China).…”

Section: Serum Metabolomics Studymentioning

confidence: 99%

“…The metabolome is downstream of the network of the gene regulatory and protein action and provides terminal information of biology . Urinary metabolomics is applied to investigate the antifatigue effects of SAL; intracellular metabolomics is employed in the investigation of the ameliorating effect of SAL on hypoxic injury; liver metabolomics is conducted to discuss how SAL protects against furan-induced toxicity in mice . Hence, we used serum metabolomics for the investigation of the alleviating effects of SAL against furan-induced toxicity by investigating potential biomarkers and the metabolic pathways to which the biomarkers belong.…”

Section: Introductionmentioning

confidence: 99%

“…23 Urinary metabolomics is applied to investigate the antifatigue effects of SAL; 24 intracellular metabolomics is employed in the investigation of the ameliorating effect of SAL on hypoxic injury; 25 liver metabolomics is conducted to discuss how SAL protects against furan-induced toxicity in mice. 26 Hence, we used serum metabolomics for the investigation of the alleviating effects of SAL against furaninduced toxicity by investigating potential biomarkers and the metabolic pathways to which the biomarkers belong. Our previous research has validated the role of SAL in alleviating liver injury, systemic inflammation, and intestinal microflora dysregulation induced by furan.…”

Section: Introductionmentioning

confidence: 99%

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Modulation of Disordered Bile Acid Homeostasis and Hepatic Tight Junctions Using Salidroside against Hepatocyte Apoptosis in Furan-Induced Mice

Wang

Liu

et al. 2022

J. Agric. Food Chem.

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Furan, a processing-induced food contaminant, has attracted great attention due to its hepatotoxicity. To further investigate the underlying mechanism of salidroside (SAL) alleviating furan-induced liver damage, we divided Balb/c mice into the control group, the furan (8 mg/kg/day) group, and three groups of three different doses of SAL (10/20/40 mg/kg/day) in the current research. The shifted serum profile was observed through untargeted metabonomics, to which the bile acid metabolism was related, and the alleviating effect of SAL against furan-induced apoptosis was caused by the metabolism. Target bile acid quantification for the liver and serum showed that SAL positively regulated the homeostasis of bile acids disturbed by furan. Meanwhile, SAL significantly upregulated the synthesis genes of bile acids (Cyp7a1, Cyp7b1, Cyp8b1, and Cyp27a1) and the uptake transport genes (Ntcp and Oatps) and downregulated the efflux transport genes (Bsep, Ost-α, Ost-β, Mrp2, and Mrp4). Transmission electron microscopy of the bile canaliculi and tight junctions and the levels of tight junction marker proteins (ZO-1, occludin, and claudin-1) confirmed that the disruption of the hepatic tight junction was inhibited by SAL. The connection between the apoptosis- and tight junction-related proteins was observed through the construction of a protein–protein interaction network. SAL suppressed the furan-induced hepatocyte apoptosis evidenced by the detection of TUNEL and Bax, Bcl-2, and caspase-3 levels. Taken together, SAL alleviated furan-induced hepatocyte apoptosis via altering the disordered homeostasis of bile acids and hepatic tight junctions.

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Section: Discussionmentioning

confidence: 94%

Section: Serum Metabolomics Studymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Modulation of Disordered Bile Acid Homeostasis and Hepatic Tight Junctions Using Salidroside against Hepatocyte Apoptosis in Furan-Induced Mice

Wang

Liu

et al. 2022

J. Agric. Food Chem.

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“…This phenomenon probably affects absorption during digestion or metabolism by the liver because CPT-11 increases drug toxicity to induce IIM. Furan is another metabolite affecting hepatic metabolism by dysregulating the biosynthesis of primary bile acids ( Yuan et al, 2020 ) and injury due to free radical-mediated lipid peroxidation ( Yuan et al, 2013 ), which was increased to promote IIM ( Figure 5A ).…”

Section: Discussionmentioning

confidence: 99%

Systematic evaluation of irinotecan-induced intestinal mucositis based on metabolomics analysis

Zhang²,

Zhao³

et al. 2022

Front. Pharmacol.

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Chemotherapy-induced intestinal mucositis (CIM) is a major dose-limiting side effect of chemotherapy, especially in regimens containing irinotecan . Several studies on the pathologic mechanisms of CIM focused on both the genomics and molecular pathways triggered by chemotherapy. However, systematic evaluation of metabolomic analysis in irinotecan-induced intestinal mucositis (IIM) has not been investigated. This study aimed to comprehensively analyze metabolite changes in main tissues of IIM mouse models. Male ICR mice were assigned to two groups: the model group (n = 11) treated with CPT-11 (20 mg/kg daily; i.p.) and the control group (n= 11) with solvent for 9 days. Gas chromatography-mass spectrometry (GC-MS) was used to investigate the metabolic alterations in the serum, intestinal, colonic, hepatic, and splenic samples of mice between two groups by multivariate statistical analyses, including GC-MS data processing, pattern recognition analysis, and pathway analysis. Forty-six metabolites, including hydrocarbons, amino acids, lipids, benzenoids, hydroxy acids, and amines, had significant changes in levels in tissues and sera of IIM mouse models. The most important pathways related to the identified metabolites were the glycerolipid metabolism in the colon and aminoacyl-tRNA biosynthesis; glycine, serine, and threonine metabolism; and glyoxylate and dicarboxylate metabolism in the liver. Our study firstly provided a comprehensive and systematic view of metabolic alterations of IIM using GC-MS analysis. The characterizations of metabolic changes could offer profound and theoretical insight into exploring new biomarkers for diagnosis and treatment of IIM.

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Potential herb–drug interactions between anti-COVID-19 drugs and traditional Chinese medicine

Ye¹,

Fan²,

Zhao³

et al. 2023

Acta Pharmaceutica Sinica B

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Salidroside ameliorates liver metabonomics in relation to modified gut-liver FXR signaling in furan-induced mice

Cited by 14 publications

References 40 publications

Modulation of Disordered Bile Acid Homeostasis and Hepatic Tight Junctions Using Salidroside against Hepatocyte Apoptosis in Furan-Induced Mice

Modulation of Disordered Bile Acid Homeostasis and Hepatic Tight Junctions Using Salidroside against Hepatocyte Apoptosis in Furan-Induced Mice

Systematic evaluation of irinotecan-induced intestinal mucositis based on metabolomics analysis

Potential herb–drug interactions between anti-COVID-19 drugs and traditional Chinese medicine

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