Salidroside Affects Gut Microbiota Structure in db/db Mice by Affecting Insulin, Blood Glucose and Body Weight

Abstract

The purpose of this study was to investigate the regulatory effect of salidroside on the intestinal flora of mice with type 2 diabetes (T2DM) and its protective effect in the body. Patients and Methods:We acclimated 8-week-old mice for 7 days, divided them into 4 groups, and continued dosing for 8 weeks. We recorded weekly blood glucose levels and body weight for each mouse. After the completion of the feeding cycle, the 16S rRNA of the intestinal flora in the mice was sequenced, and the insulin and C-peptide levels in each group of mice were measured. Four samples were taken from each group for liver and kidney section staining. Results: Our results showed that gut microbiota diversity and function were significantly different between the diabetic mice and healthy mice and that insulin levels, body weight, and blood glucose levels could significantly influence gut microbiota changes at the genus level. The gut microbiota diversity and function of db/db mice were also altered after salidroside administration. Salidroside could attenuate inflammatory damage, lipid accumulation and inflammatory changes in the diabetic liver, as well as diabetic kidney damage. Candidatus arthromitus and Odoribacter are important species of the microbiota during diabetes and may serve as potential therapeutic targets. Conclusion: Our investigation of the associated pathological conditions and fecal microbiota in db/db mice provides new insights into the pathogenesis of T2DM and provides implications for the diagnosis and treatment of T2DM.

Unraveling intestinal microbiota’s dominance in polycystic ovary syndrome pathogenesis over vaginal microbiota

“…Lactobacillus transplantation and administration can improve the symptoms of PCOS, which is conducive to the treatment of PCOS ( Guo et al., 2016 ; Siddiqui et al., 2022 ). Candidatus Arthromitus is involved in body glucose metabolism and is positively correlated with body weight ( Fu et al., 2022 ; Zhao et al., 2022 ). Our study found that T was significantly positively correlated with Enterorhabdus , LH was significantly positively correlated with Lactobacillus , and body weight was significantly positively correlated with Ligilactobacillus , Candidatus Arthromitus .…”

Potential efficacy and mechanism of eight mild-natured and bitter-flavored TCMs based on gut microbiota: A review

“…In both Furan-induced liver injury and high-fat diet (HFD)-induced non-alcoholic steatohepatitis mice, salidroside decreased serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and proinflammatory factor, accompanied by the enhancement of Akkermansia and Roseburia , and decline of Sporobacter , Blautia , Desulfovibrio , Anaerofustis , Olsenella , Bifidobacterium , Vasilyevaea, Lactobacillus and Alloprevotella ( Li et al, 2020 , Yuan et al, 2019 ) . Furthermore, salidroside could reduce the glucose level and protect heart, liver, kidney from diabetes-caused damage, which at least partly mediated by the alteration of gut microbiota, such as the increase of Prdiococcus , Alloprevotella , and decrease of Enterobacter , Lactobacillus , Mycoplasm a, Enterococcus , Candidatus Arthromitus , and Lachnospiraceae UCG-006 ( Shi et al, 2022 , Zhao et al, 2022 ). These results will shed a new light on the future of the targeted and precise modulation of microbiota to treat diabetes mellitus.…”

Effects of Modified Dietary Fiber from Fresh Corn Bracts on Obesity and Intestinal Microbiota in High-Fat-Diet Mice

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