Salicylic acid‐inducible binding of a tobacco nuclear protein to a 10 bp sequence which is highly conserved amongst stress‐inducible genes

Abstract

A 10 bp sequence motif (TCATCTTCTT) which is repeated several times in the 5' non-transcribed region of a barley beta-1,3-glucanase gene is also present in the non-translated regions of over 30 different plant genes which are known to be induced by one or more forms of stress. Gel retardation assays and South-western blotting experiments provide evidence that the motif is the binding site for a tobacco nuclear protein with an apparent molecular weight of 40 kDa. Binding activity is increased when nuclear extracts from salicylic acid-treated plants are analysed compared with extracts from control plants, indicating that the protein itself is either induced or modified under conditions of stress. These observations suggest roles for the 10 bp motif and its binding protein as cis- and trans-acting regulators of gene expression during response to stress.

Engineering disease resistance in plants

“…The complete sequence of the 2.3 kb genomic clone was used in a BLAST homology search against the EMBL database. Two clones displaying more than 99% homology were found in the sequences of BAC MZB10 (Lin et al, 1999;AC009326) et al, 1989)), H = H-box (consensus is CCTACC, Lois et al, 1989), W = W-box (core of motif is nTGACn, Eulgem et al, 2000), NF-кB represents a putative binding site for NF-кB , TCA is motif known to bind tobacco nuclear proteins upon SA treatment (Goldsbrough et al, 1993) and ATG is the translation start of MZB10.4.…”

“…NF-κB motifs are found in the salicylic acid and pathogen inducible Arabidopsis PR-1 promoter and promoters of genes upregulated in the constitutive SAR Arabidopsis mpk4 mutant (Petersen et al, 2000). The TCA motif at position 366-375 is found in many SA induced genes and binds a nuclear protein in tobacco upon SA induction (Goldsbrough et al, 1993). Since activation of a pathogen inducible promoter under other conditions than pathogen attack is unfavourable we analysed the 488 promoter sequence for the presence of cis-acting elements involved in the abiotic stress response.…”

“…A relatively small number of these promoters have been studied extensively and several different regulatory elements in these promoters have been identified Fischer, 1994;Petersen et al, 2000). Consensus sequences for different elements that may play a role in the regulation of the response of these promoters to pathogen attack have been described (Lois et al, 1989;Loake et al, 1992;Goldsbrough et al, 1993;Fischer, 1994;Dröge-Laser et al, 1997;Rushton and Somssich, 1998;Takeda et al, 1999;Yamamoto et al, 1999).…”

Differential Expression of Chitinases in Vitis vinifera L. Responding to Systemic Acquired Resistance Activators or Fungal Challenge

“…In view of the lack of a particular physiological function, the common features of classification recognized early were the protease resistance and stress inducibility (Ohashi and Oshima, 1992). However, the effective induction of PRPs was also accomplished by low concentrations of SA (Pierpoint et al, 1990), suggesting the conservation of SA-responsive, cis-active promoter elements (Goldsbrough et al, 1993). Plant Physiol.…”

Ensnaring microbes: the components of plant disease resistance

“…toxicity to microbes is also possible since HjOj kills microbes at concentrations known to be produced in planta (Peng & Kuc, 1992;Svalheim & Robertson, 1993), However, only one study provides convincing evidence for a direct role for HjOj in resistance. The expression of a H2O2-generating glucose oxidase from Aspergillus niger in the apoplast of transgenic potato resulted in good resistance to the bacterial soft rot pathogen Erwinia carotovora subsp, catotovora, as well as enhanced resistance to the fungus Phytophthora infestans (Wu et al, 1995), Although S A is required by several R genes for the manifestation of resistance , its exact role in the defence response remains unclear (Raskin, 1992), Exogenous SA application to numerous plant species induces the co-ordinated expression of a subset of PR genes , Several of these PR genes have a salicyclic acid responsive site, the AS-1 element (TCATCTTCTT), within their promoters (Goldsborough, Albrecht & Stratford, 1993;Horvaith & Chua, 1994), Thus an Avr-R* (activated) pathway involving SA might induce the expression of an array of PR genes around the site of genetic incompatibility. An example of localized PR gene expression in R-^i;T-mediated resistance is reported by Ashfield et aL, (1994), for the tomato-C.fulvum interaction.…”