SalI restriction endonuclease maps of FII incompatibility group R plasmids

Barton, Carolyn R.; Warren, Richard L.; Jezo, P.; Easton, Alan M.; Rownd, Robert H.

doi:10.1016/0147-619x(79)90013-1

Cited by 15 publications

(10 citation statements)

References 10 publications

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“…The general structures of the r-determinant components of other IncFII plasmids are similar to that of NR1 (2,10,25,158,167,175). R6 contains a kanamycin resistance determinant inserted into EcoRI-J, and NR84 contains an ampicillin resistance determinant inserted into EcoRI-L (10).…”

Section: Antibiotic Resistance Determinantsmentioning

confidence: 77%

“…They were able to map the locations of 11 tra cistrons, confirmed the order of the resistance genes, and indicated that mercuric ion resistance (mer) was located between repA in the RTF and sul in the r-determinants (32). A physical map of the 13 largest EcoRI restriction endonuclease fragments of NR1 was constructed (167), followed by similar maps for the EcoRI and Sall fragments of plasmids NR1, NR84, and R6 (10). By analysis of the properties of clones of each of the 13 largest EcoRI fragments from NR1 and the knowledge of their locations on the NR1 map, the physical position of each of the antibiotic resistance genes of NR1 was determined (80, 98).…”

Section: Genetic and Physical Mapping Of Incfiimentioning

confidence: 95%

“…The insertion includes transposon Tn903 and confers resistance to kanamycin (10,53,79,88). Tn9O3 contains one HindIII site (53), and the additional DNA sequences in the insertion that are clockwise from Tr9O3 contain one SalI site (10), which result in different restriction maps for plasmids NR1 and R6 (10,142,175). R6 also contains a 1.8-kb insertion at position 30.2 kb of NR1.…”

Section: Comparison Of the Nr1 Map With Other Plasmidsmentioning

confidence: 99%

“…All of these derivatives apparently are transfer proficient. The final known difference between R6 and NR1 is that R6 lacks the Sall site present at position 87.1 kb of NR1 (10).…”

Section: Comparison Of the Nr1 Map With Other Plasmidsmentioning

confidence: 99%

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Genetic and physical map of plasmid NR1: comparison with other IncFII antibiotic resistance plasmids.

Womble¹,

Rownd²

1988

Microbiological Reviews

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Section: Antibiotic Resistance Determinantsmentioning

confidence: 77%

Section: Genetic and Physical Mapping Of Incfiimentioning

confidence: 95%

Section: Comparison Of the Nr1 Map With Other Plasmidsmentioning

confidence: 99%

“…All of these derivatives apparently are transfer proficient. The final known difference between R6 and NR1 is that R6 lacks the Sall site present at position 87.1 kb of NR1 (10).…”

Section: Comparison Of the Nr1 Map With Other Plasmidsmentioning

confidence: 99%

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Genetic and physical map of plasmid NR1: comparison with other IncFII antibiotic resistance plasmids.

Womble¹,

Rownd²

1988

Microbiological Reviews

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“…The host for the RSF2124::R100 recombinant plasmids was E. coli KP245 (20) with the exceptions of plasmids pRR104 and pDW4, whose hosts were E. coli JG112 and C600, respectively (21). The plasmid R100* is our laboratory stock of R100 (32) which was found to contain an uncharacterized insertion of 1.4 kilobase pairs in EcoRI fragment F, at 72 min on the R100 map (3,19,30). Plasmid-bearing strains were constructed by liquid matings in the case of R100 and its derivatives, or by transformation (27) in the case of nonconjugative plasmids.…”

Section: Methodsmentioning

confidence: 99%

dnaB analog function associated with plasmid R100drd-1

Potter¹,

Iyer²

1983

J Bacteriol

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Plasmid R100 and a number of its derivatives were able to suppress the temperature sensitivity of strains carrying different alleles of the dnaB gene of Escherichia coli K-12. R1O0drd-J and pAR132 were able to rescue a strain carrying the dnaB266(Am) mutation in the absence of any known amber suppressors. This was taken as evidence for the existence of an R1OOdrd-J dnaB analog function. The R1OOdrd-J dnaB analog was different from those of bacteriophages P1 and P7 in that it was able to support the growth of bacteriophage lambda in a dnaB266(Am) background. The dnaB analog was also shown to be thermosensitive. The structural gene for this protein lies within the EcoRI fragment D of R1OOdrd-1.

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Lambda transducing phages derived from a finO- R100::λ cointegrate plasmid: Proteins encoded by the R100 replication/incompatibility region and the antibiotic resistance determinant

Dempsey

McIntire

1979

Molec. gen. Genet.

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Three lambda transducing phages have been isolated from pEDR20, an R100::lambda cointegrate plasmid in which the lambda insertion inactivated the R100 finO gene. Physical analysis of the three phages showed that the lambda is inserted at kilobase coordinate 81.3 of R100. All three phages carry different amounts of R100 DNA in the left arm of lambda. Each pahge contains ISlb, the mer genes and the region between coordinate 81.3 and 88.6; thus, all contain the genes necessary for R100 replication. One phage, VA lambda 73, contains the entire r-determination of R100 in addition to the above DNA. Five proteins coded by the region between 81.3 and 88.6 were detected. These had subunit molecular weights of 10,400; 12,200; 16,200; 19,600; and 38,300. The first was made constitutively and the other four only from a lambda promoter. Other constitutive proteins were one from the cml fus region with a molecular weight of 22,400 (cml) and two from the str sul region with molecular weights of 31,500 (str?) and 30,100 (sul?). Mercuric ion induced synthesis of at least 10 proteins. Six of these were known from earlier work. The total size of the proteins which appear to derive from the mer genes exceeds by a factor of 1.5, the coding capacity of this region without overlapping genes. Some, or all of these extra proteins may be chromosomal in origin, possibly derepressed in response to mercury gene products.

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SalI restriction endonuclease maps of FII incompatibility group R plasmids

Cited by 15 publications

References 10 publications

Genetic and physical map of plasmid NR1: comparison with other IncFII antibiotic resistance plasmids.

Genetic and physical map of plasmid NR1: comparison with other IncFII antibiotic resistance plasmids.

dnaB analog function associated with plasmid R100drd-1

Lambda transducing phages derived from a finO- R100::λ cointegrate plasmid: Proteins encoded by the R100 replication/incompatibility region and the antibiotic resistance determinant

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