Sakuranetin interacting with cell membranes models: Surface chemistry combined with molecular simulation

Pires, Guilherme Henrique da Cruz Ramos; Freire, Vitor Torres; Pereira, Rafael Guimarães; Siqueira, Leonardo José Amaral de; Umehara, Eric; Lago, João Henrique G.; Caseli, Luciano

doi:10.1016/j.colsurfb.2022.112546

Cited by 2 publications

(1 citation statement)

References 48 publications

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“…Sakuranetin is a lipophilic compound that has been shown to be incorporated in cell membranes 45 . To test whether sakuranetin treatment could modify membrane rigidity and slow down the formation of endocytic vesicles, we assessed the recoveries of fluorescence intensity by using a fluorescence recovery after photobleaching (FRAP) method in rice root PM treated with exogenous and higher or lower endogenous sakuranetin.…”

Section: Resultsmentioning

confidence: 99%

Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice blast

Jiang,

Zhang,

Zhao

et al. 2024

Nat Commun

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Phytoalexin sakuranetin functions in resistance against rice blast. However, the mechanisms underlying the effects of sakuranetin remains elusive. Here, we report that rice lines expressing resistance (R) genes were found to contain high levels of sakuranetin, which correlates with attenuated endocytic trafficking of plasma membrane (PM) proteins. Exogenous and endogenous sakuranetin attenuates the endocytosis of various PM proteins and the fungal effector PWL2. Moreover, accumulation of the avirulence protein AvrCO39, resulting from uptake into rice cells by Magnaporthe oryzae, was reduced following treatment with sakuranetin. Pharmacological manipulation of clathrin-mediated endocytic (CME) suggests that this pathway is targeted by sakuranetin. Indeed, attenuation of CME by sakuranetin is sufficient to convey resistance against rice blast. Our data reveals a mechanism of rice against M. oryzae by increasing sakuranetin levels and repressing the CME of pathogen effectors, which is distinct from the action of many R genes that mainly function by modulating transcription.

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Section: Resultsmentioning

confidence: 99%

Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice blast

Jiang,

Zhang,

Zhao

et al. 2024

Nat Commun

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Distinctive outlook into the mechanism of resistance to rice blast

Tan,

Wu,

Men

2024

Phytopathol Res

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Rice blast, caused by the filamentous fungus Magnaporthe oryzae (also known as Pyricularia oryzae), is the most severe disease threatening rice yield. Sakuranetin, an important plant phytoalexin, exhibits inhibitory effects on rice blast fungus growth. However, the underlying molecular mechanism by which sakuranetin enhances plant resistance against pathogens remains unclear. A recent study published in Nature Communications by Jiang et al. (Nat. Commun, 2023, https://doi.org/10.1038/s41467-024-47746-y) elucidated from a cytological perspective how sakuranetin enhances resistance to rice blast disease. The research revealed that elevated accumulation of sakuranetin attenuates the endocytosis of M. oryzae effectors into the cytoplasm of rice host cells. The inhibitory effect of sakuranetin is dosage-dependent and targets clathrin-mediated endocytosis (CME). This study provides novel insights into understanding plant immune mechanisms underlying weakening pathogen virulence weapons.

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Sakuranetin interacting with cell membranes models: Surface chemistry combined with molecular simulation

Cited by 2 publications

References 48 publications

Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice blast

Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice blast

Distinctive outlook into the mechanism of resistance to rice blast

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