Sake Lees Fermented with Lactic Acid Bacteria Prevents Allergic Rhinitis-Like Symptoms and IgE-Mediated Basophil Degranulation

Kawamoto, Seiji; Kaneoke, Mitsuoki; Ohkouchi, Kayo; Amano, Yuichi; Takaoka, Yuki; Kume, Kazunori; Aki, Tsunehiro; Yamashita, Susumu; Watanabe, Kenji; Kadowaki, Motoni; Hirata, Dai; Ono, Kazuhisa

doi:10.1271/bbb.100541

Cited by 24 publications

(15 citation statements)

References 15 publications

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“…MC/9 cells, a murine mast cell clone derived from mouse fetal liver (American Type Culture Collection, Manassas, VA, USA, catalogue number: CRL‐8306), were employed for the β‐hexosaminidase assay. Culture conditions and methods of the β‐hexosaminidase assay were described in detail previously …”

Section: Methodsmentioning

confidence: 99%

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Critical micelle concentration and particle size determine adjuvanticity of cyclic lipopeptides

et al. 2018

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Cyclic lipopeptides such as surfactin and polymyxin have potent mucosal adjuvant properties. Cyclic lipopeptides are tensioactive compounds but the relationship between adjuvanticity and surface activity is unknown. Here, we show that the critical micelle concentration (cmc) of surfactant and particle size of the surfactant-protein complex are important determinants of cyclic lipopeptide adjuvanticity. We found that the diameter of cyclic lipopeptide-ovalbumin (OVA) complex particles was significantly larger than that in the solutions of OVA alone at cyclic lipopeptide concentrations above the cmc. OVA-specific antibody titers in mice immunized intranasally with OVA and a cyclic lipopeptide at concentrations above its cmc were significantly higher than those in mice immunized with OVA plus the same dose of the cyclic lipopeptide but administered with formulations in which cyclic lipopeptide concentration was below the cmc. Thus, the concentration of the cyclic lipopeptide in the formulation at immunization, but not its overall dose, was critical for its adjuvanticity. Furthermore, two types of aggregates, the cyclic lipopeptide simplex micelles and the cyclic lipopeptide-OVA complex micelles, were found in formulations with SF concentrations above its cmc. Degranulation of mast cells exposed to SF simplex micelles was more pronounced when SF concentration was above the cmc. In conclusion, our study showed that surface activity properties, such as the cmc and the size of surfactant-protein complex contribute to the adjuvanticity of cyclic lipopeptides. Our study proposes a novel idea that cmc is a key parameter for tensioactive adjuvants. This article is protected by copyright. All rights reserved.

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Section: Methodsmentioning

confidence: 99%

“…Culture conditions and methods of the β-hexosaminidase assay were described in detail previously. 14,36…”

Section: β-Hexosaminidase Assaymentioning

confidence: 99%

Critical micelle concentration and particle size determine adjuvanticity of cyclic lipopeptides

et al. 2018

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“…For a degranulation assay, MC/9 cells (2×10 5 cells/200 µL per well) were cultured with or without various dosages of PMB, CL, PMBN, CLMS, and compound 48/80 (a positive control; Sigma-Aldrich) for 30 min in 96-well microplates containing Tyrode's salt solution (Sigma-Aldrich). After centrifugation, supernatants were collected and degranulation was determined by a β-hexosaminidase assay as described previously [37], [38]. Briefly, supernatant samples were incubated at 37°C for 60 min with 1 mM 4-nitrophenyl N -acetyl-β-D-glucosaminide (Sigma-Aldrich) in a 0.1 M citric acid buffer (pH 4.5).…”

Section: Methodsmentioning

confidence: 99%

“…The release of total intracellular mediators was achieved by cell lysis with 0.2% Triton X-100 in Tyrode's salt solution. The percentages of released β-hexosaminidase were calculated by a formula described elsewhere [38]. Concentrations of histamine in each supernatant sample were determined by a histamine EIA kit (SPI-Bio, Montigny le Bretonneux, France).…”

Section: Methodsmentioning

confidence: 99%

Polymyxins as Novel and Safe Mucosal Adjuvants to Induce Humoral Immune Responses in Mice

et al. 2013

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There is currently an urgent need to develop safe and effective adjuvants for enhancing vaccine-induced antigen-specific immune responses. We demonstrate here that intranasal immunization with clinically used polypeptide antibiotics, polymyxin B (PMB) and colistin (CL), along with ovalbumin (OVA), increases OVA-specific humoral immune responses in a dose-dependently manner at both mucosal and systemic compartments. Enhanced immunity by boosting was found to persist during 8 months of observation. Moreover, mice intranasally immunized with OVA plus various doses of PMB or CL showed neither inflammatory responses in the nasal cavity and olfactory bulbs nor renal damages, compared to those given OVA alone. These data suggest that polymyxins may serve as novel and safe mucosal adjuvants to induce humoral immune responses. The polymyxin adjuvanticity was found to be independent of endotoxins liberated by its bactericidal activity, as indicated by similar enhancing effects of PMB in lipopolysaccharide (LPS)-hyporesponsive and LPS-susceptible mice. However, despite the presence of preexisting anti-PMB antibodies, we observed no reduction in the adjuvant function of polymyxins when they were given intranasally. Furthermore, the titers of OVA-specific Abs in mice intranasally immunized with OVA plus PMB or CL were significantly higher than those in mice administered with polymyxin analogues, such as polymyxin B nonapeptide and colistin methanesulfonate. The levels of released β-hexosaminidase and histamine in mast cell culture supernatants stimulated by PMB or CL were also significantly higher than those stimulated by their analogues. These results suggest that both the hydrophobic carbon chain and hydrophilic cationic cyclic peptide contribute to the mucosal adjuvanticity of PMB and CL.

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“…Due to the 'common mucosal response' concept, which introduces the idea that the alterations in immune function of the respiratory tract are linked to the immunomodulatory activity of the gut microbiome, oral administration of probiotics was used for treating allergic airway diseases by changing the gut microbiome. Some investigations have shown that the oral administration of probiotics and prebiotics may benefit allergic rhinitis patients (95)(96)(97). Moreover, the local nasal administration of Lactococcus lactis NZ9000 can affect the local and systemic immune responses against Streptococcus pneumonia (98).…”

Section: Perspectivesmentioning

confidence: 99%

Viruses and bacteria in Th2‐biased allergic airway disease

Feng

Zhang

Gevaert

et al. 2016

Allergy

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Allergic airway diseases are typically characterized by a type 2-biased inflammation. Multiple distinct viruses and bacteria have been detected in the airways. Recently, it has been confirmed that the microbiome of allergic individuals differs from that of healthy subjects, showing a close relationship with the type 2 response in allergic airway disease. In this review, we summarize the recent findings on the prevalence of viruses and bacteria in type 2-biased airway diseases and on the mechanisms employed by viruses and bacteria in propagating type 2 responses. The understanding of the microbial composition and postinfectious immune programming is critical for the reconstruction of the normal microflora and immune status in allergic airway diseases.

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Sake Lees Fermented with Lactic Acid Bacteria Prevents Allergic Rhinitis-Like Symptoms and IgE-Mediated Basophil Degranulation

Cited by 24 publications

References 15 publications

Critical micelle concentration and particle size determine adjuvanticity of cyclic lipopeptides

Critical micelle concentration and particle size determine adjuvanticity of cyclic lipopeptides

Polymyxins as Novel and Safe Mucosal Adjuvants to Induce Humoral Immune Responses in Mice

Viruses and bacteria in Th2‐biased allergic airway disease

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