Sak 57, an intermediate filament keratin present in intercellular bridges of rat primary spermatocytes

Tres, Laura L.; Rivkin, Eugene; Kierszenbaum, Abraham I.

doi:10.1002/(sici)1098-2795(199609)45:1<93::aid-mrd13>3.0.co;2-y

Cited by 23 publications

(8 citation statements)

References 49 publications

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“…9). As such, the immunological barrier can be maintained during the transit of spermatocytes at the BTB even though these cells are connected in clones by intercellular bridges (Fawcett et al, 1959;Fawcett, 1961;Weber and Russell, 1987;Tres et al, 1996;Kierszenbaum, 2002;Hamer et al, 2003;Greenbaum et al, 2006;Chang et al, 2010;Hermo et al, 2010).…”

Section: Drug Transporters and Blood-testis Barrier Dynamics: Recementioning

confidence: 99%

The Blood-Testis Barrier and Its Implications for Male Contraception

2011

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Section: Drug Transporters and Blood-testis Barrier Dynamics: Recementioning

confidence: 99%

The Blood-Testis Barrier and Its Implications for Male Contraception

2011

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“…Of the six known or suspected components of the intercellular bridge, five are broadly expressed cytoskeletal components that localize to multiple structures (27,(29)(30)(31)(32). HSF2 is unique because it is the only noncytoskeletal bridge component (28).…”

Section: Tex14 Is a Component Of The Intercellularmentioning

confidence: 99%

TEX14 is essential for intercellular bridges and fertility in male mice

Greenbaum

Yan²,

Wu³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

249

276

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Cytokinesis in somatic cells concludes with the formation of a midbody, which is abscised to form individual daughter cells. In contrast, germ cell cytokinesis results in a permanent intercellular bridge connecting the daughter cells through a large cytoplasmic channel. During spermatogenesis, proposed roles for the intercellular bridge include germ cell communication, synchronization, and chromosome dosage compensation in haploid cells. Although several essential components of the midbody have recently been identified, essential components of the vertebrate germ cell intercellular bridge have until now not been described. Herein, we show that testis-expressed gene 14 (TEX14) is a novel protein that localizes to germ cell intercellular bridges. In the absence of TEX14, intercellular bridges are not observed by using electron microscopy and other markers. Spermatogenesis in Tex14 ؊/؊ mice progresses through the transit amplification of diploid spermatogonia and the expression of early meiotic markers but halts before the completion of the first meiotic division. Thus, TEX14 is required for intercellular bridges in vertebrate germ cells, and these studies provide evidence that the intercellular bridge is essential for spermatogenesis and fertility.cytoplasmic bridges ͉ knockout mouse ͉ male infertility ͉ male sterility ͉ ring canals

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“…This may be due the proteins found inside the cytoplasmic bridge. In mammals only few proteins have been demonstrated in the walls of the cytoplasmic bridges: cytoskeletal proteins actin , sak 57 (Tres et al, 1996), and HSF-2 (Alastalo et al, 1998). However, molecular structure and the development of intercellular bridges in Drosophila germ cells are better understood (Robinson and Cooley, 1996).…”

Section: Active Role Of Cytoplasmic Bridges In Gene Product Sharingmentioning

confidence: 99%

Intercellular Organelle Traffic through Cytoplasmic Bridges in Early Spermatids of the Rat: Mechanisms of Haploid Gene Product Sharing

Ventelä

Toppari²,

Parvinen³

2003

MBoC

187

159

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Stable cytoplasmic bridges (or ring canals) connecting the clone of spermatids are assumed to facilitate the sharing of haploid gene products and synchronous development of the cells. We have visualized these cytoplasmic bridges under phase-contrast optics and recorded the sharing of cytoplasmic material between the spermatids by a digital time-lapse imaging system ex vivo. A multitude of small (ca. 0.5 m) granules were seen to move continuously over the bridges, but only 28% of those entering the bridge were actually transported into other cell. The average speed of the granules decreased significantly during the passage. Immunocytochemistry revealed that some of the shared granules contained haploid cell-specific gene product TRA54. We also demonstrate the novel function for the Golgi complex in acrosome system formation by showing that TRA54 is processed in Golgi complex and is transported into acrosome system of neighboring spermatid. In addition, we propose an intercellular transport function for the male germ cellspecific organelle chromatoid body. This mRNA containing organelle, ca. 1.8 m in diameter, was demonstrated to go over the cytoplasmic bridge from one spermatid to another. Microtubule inhibitors prevented all organelle movements through the bridges and caused a disintegration of the chromatoid body. This is the first direct demonstration of an organelle traffic through cytoplasmic bridges in mammalian spermatogenesis. Golgi-derived haploid gene products are shared between spermatids, and an active involvement of the chromatoid body in intercellular material transport between round spermatids is proposed.

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Sak 57, an intermediate filament keratin present in intercellular bridges of rat primary spermatocytes

Cited by 23 publications

References 49 publications

The Blood-Testis Barrier and Its Implications for Male Contraception

The Blood-Testis Barrier and Its Implications for Male Contraception

TEX14 is essential for intercellular bridges and fertility in male mice

Intercellular Organelle Traffic through Cytoplasmic Bridges in Early Spermatids of the Rat: Mechanisms of Haploid Gene Product Sharing

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