SAGE of the developing wheat caryopsis

McIntosh, Shane; Watson, Loraine; Bundock, Peter C; Crawford, Allison C; White, Jim; Cordeiro, Giovanni M; Barbary, Daniel; Rooke, Lee; Henry, Robert J.

doi:10.1111/j.1467-7652.2006.00218.x

Cited by 50 publications

(47 citation statements)

References 63 publications

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“…PINA protein has been detected at 20 DAF (the earliest time point examined; Dubreil et al 1998), increasing to 40 DAF, then plateauing; later studies using enzyme-linked immunosorbent assays (ELISA) could detect PINA protein at 10 DAF and PINB at 15 DAF (Turnbull et al 2003a). The transcriptome analyses of developing wheat seeds appears to be particularly sensitive in detecting expressions at very early time points, for example, from 3 to 9 DPA (Clarke and Rahman 2005;Drea et al 2005;Laudencia-Chingcuanco et al 2007;McIntosh et al 2007). Laudencia-Chingcuanco et al (2007) identified Gsp-1 amongst the genes most differentially expressed and involved in grain filling, along with metabolic or defence functions (e.g., storage proteins, alpha-amylase inhibitors) and expression of PINs and defence proteins correlated with dry weight accumulation.…”

Section: Regulation Of Expression Of Puroindoline and Gsp-1 Genesmentioning

confidence: 98%

“…Of particular interest is the fact that a number of ns-LTPs exhibit antibacterial, antifungal or antiviral activities, some isoforms being up-regulated under pathogen infections or involved in signalling pathways (Kader 1996;Garcia-Almedo et al 1998;Blein et al 2002;Cameron et al 2006). The nsLTPs (and other defence-related proteins) are expressed at early stages of seed development (Boutrot et al 2005;Drea et al 2005;McIntosh et al 2007).…”

Section: Promoter Sequences In Diploid Progenitors Of Wheat and Wild mentioning

confidence: 99%

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Molecular genetics of puroindolines and related genes: regulation of expression, membrane binding properties and applications

2007

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Kernel hardness is mainly conditioned by allelic variations of Pina-D1 and Pinb-D1 genes. The Ecotilling approach was optimized to investigate Pina and Pinb alleles in the micro-core collections (MCC) of Chinese wheat germplasm. As a result, three Pina and eight Pinb alleles were found. A novel variant (designated as Pinb-D1x) was discovered in one of the accessions from Xinjiang winter-spring wheat region. Generally, more Pinb alleles were detected in the accessions coming from the regions that grow winter or a mixture of spring and winter wheats.

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Section: Regulation Of Expression Of Puroindoline and Gsp-1 Genesmentioning

confidence: 98%

Section: Promoter Sequences In Diploid Progenitors Of Wheat and Wild mentioning

confidence: 99%

Molecular genetics of puroindolines and related genes: regulation of expression, membrane binding properties and applications

2007

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“…On the other hand, with open systems there is no need for previous knowledge of the genome or transcriptome of the organism. cDNA-AFLP (cDNA-Amplified fragment length polymorphism), MPSS (massively parallel signature sequencing), and specially SAGE (serial analysis of gene expression) have been successfully used to quantify transcript abundance and generate expression data across different tissue types or developmental stages in higher plants ( Fizames ( Fizames et al, 2004;Meyers et al, 2004;Calsa and Figueira, 2007;Chen et al, 2007;Leymarie et al, 2007;McIntosh et al, 2007;Song et al, 2007;Ritter et al, 2008). �orth mentioning are 454-sequencing .…”

Section: Techniques Used For Evaluating Gene Expression In Functionalmentioning

confidence: 99%

Biological Activity of Essential Oils from Leaves and Fruits of Pepper Tree (Schinus molle L.) to Control Rice Weevil (Sitophilus oryzae L.)

Benzi

Stefanazzi

Ferrero

2009

Chilean J. Agric. Res.

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Rice weevil (Sitophilus oryzae L.) is a primary insect pest of stored grain. The development of resistance resulted in the application of synthetic insecticides. In recent years many plant essential oils have provided potential alternatives to currently used insect control agents. The Brazilian pepper tree (Schinus molle L. var. areira (L.) DC.) (Anacardiaceae) ) (Anacardiaceae) has different biological properties such as insecticidal activity. In this study, repellent, fumigant activity, nutritional indices, and feeding deterrent action were evaluated on S. oryzae adults. Filter paper impregnation was used to test fumigant toxicity, whereas treated whole wheat was used to evaluate repellent activity and a flour disk bioassay was done to evaluate feeding deterrent action and nutritional index alteration. Leaf essential oils showed repellent effects at both concentrations (0.04 and 0.4% w/w), while fruit essential oils lacked repellent activity. Both plant oils altered nutritional indices. Fruit essential oils had a strong feeding deterrent action (62%) while leaves had a slight effect (40.6%). With respect to fumigant activity, neither of the essential oils was found to be toxic. Resistance and toxicity problems of the synthetic insecticides have resulted in the necessity of finding more effective and healthier alternatives. Thus, essential oils are the most tested products presently (Papachristos and Stamopoulus, 2002; Umoetok and Gerard, 2003; Zhang et al., 2004; Tapondjou et al., 2005; Ferrero et al., 2006; Sánchez Chopa et al., 2006, Stefanazzi et al., 2006, Wang et al., 2006. Different biological activities of plant derivatives have been demonstrated for the control of stored grain pests (Golob et al., 1999; Rajendran and Sriranjini, 2008).The Brazilian pepper tree (Schinus molle L. var. areira (L.) DC.) is associated with agricultural crops in boundaries, windbreaks, riverbank protection, and watershed conservation. In popular medicine, it is use for its astringent, diuretic, antispasmodic properties among others. In pest control, it is used as a fumigant, repellent, and ovicide (Ruffínengo et al., 2005; Ferrero et al., 2006).The objective of the present study was to evaluate the fumigant, repellent and/or attractant, feeding deterrent activity, and the alterations in the nutritional physiology produced by the essential oils from leaves and fruits of S. molle var. areira on 3-to 4-day-old S. oryzae adults. V. BENZI et al. -BIOLOGICAL ACTIVITY OF ESSENTIAL OILS… MATERIALS AND METHODS InsectsThe insects came from a susceptible colony maintained in the Cátedra de Zoología Agrícola de la Universidad Nacional de Buenos Aires (UBA). They were bred in glass containers, 8 cm in diameter, 13 cm high, sealed with fine netting, maintained at 28 ± 1 ºC, 60-70% HR, a 12:12 photoperiod, and using whole wheat grains as food. Vegetal materialThe vegetal organs (leaves and mature fruits) of the pepper tree were collected during the summer period in Bahía Blanca city, Buenos Aires, Argentina (38°41' S; 62°17' ...

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“…The main storage compounds of cereal seeds are starch and storage proteins (Crocker and Barton, 1957;van Dongen et al, 2004), which are mainly derived from Suc and amino acids and synthesized in the endosperm (Bewley and Black, 1994). Due to the agronomical importance of seeds, seed metabolism has been studied intensively with respect to biochemistry, physiology, and genetics (Finnie et al, 2004;Lai et al, 2004;Sreenivasulu et al, 2004;Ho et al, 2005;McIntosh et al, 2007) and has also been a target for metabolic engineering to improve nutritional value (Mazur et al, 1999;Ye et al, 2000;Houmard et al, 2007;Wang et al, 2007).…”

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confidence: 99%

Flux Balance Analysis of Barley Seeds: A Computational Approach to Study Systemic Properties of Central Metabolism

et al. 2008

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The accumulation of storage compounds is an important aspect of cereal seed metabolism. Due to the agronomical importance of the storage reserves of starch, protein, and oil, the understanding of storage metabolism is of scientific interest, with practical applications in agronomy and plant breeding. To get insight into storage patterning in developing cereal seed in response to environmental and genetic perturbation, a computational analysis of seed metabolism was performed. A metabolic network of primary metabolism in the developing endosperm of barley (Hordeum vulgare), a model plant for temperate cereals, was constructed that includes 257 biochemical and transport reactions across four different compartments. The model was subjected to flux balance analysis to study grain yield and metabolic flux distributions in response to oxygen depletion and enzyme deletion. In general, the simulation results were found to be in good agreement with the main biochemical properties of barley seed storage metabolism. The predicted growth rate and the active metabolic pathway patterns under anoxic, hypoxic, and aerobic conditions predicted by the model were in accordance with published experimental results. In addition, the model predictions gave insight into the potential role of inorganic pyrophosphate metabolism to maintain seed metabolism under oxygen deprivation.

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SAGE of the developing wheat caryopsis

Cited by 50 publications

References 63 publications

Molecular genetics of puroindolines and related genes: regulation of expression, membrane binding properties and applications

Molecular genetics of puroindolines and related genes: regulation of expression, membrane binding properties and applications

Biological Activity of Essential Oils from Leaves and Fruits of Pepper Tree (Schinus molle L.) to Control Rice Weevil (Sitophilus oryzae L.)

Flux Balance Analysis of Barley Seeds: A Computational Approach to Study Systemic Properties of Central Metabolism

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