SAGA–CORE subunit Spt7 is required for correct Ubp8 localization, chromatin association and deubiquitinase activity

Nuño-Cabanes, Carme; García-Molinero, Varinia; Martín-Expósito, Manuel; Gas, María-Eugenia; Oliete-Calvo, Paula; García-Oliver, Encar; Iglesia-Vayá, María de la; Rodrı́guez-Navarro, Susana

doi:10.1186/s13072-020-00367-3

Cited by 3 publications

(2 citation statements)

References 50 publications

(64 reference statements)

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“…observed in the ada1Δ and spt7Δ mutant strains. This observation is consistent with the fact that Ada1 and Spt7 belong to the CORE of SAGA and their absence has previously been shown to fully impair SAGA complex integrity(Nuño-Cabanes et al, 2020;Sterner et al, 1999;Wu & Winston, 2002). Consistently, Ada1 and Spt7 recruitment at NCR-sensitive genes were fully interdependent.…”

supporting

confidence: 90%

Differing SAGA module requirements for NCR‐sensitive gene transcription in yeast

Georis

Ronsmans

Vierendeels

et al. 2023

Yeast

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Nitrogen catabolite repression (NCR) is a means for yeast to adapt its transcriptome to changing nitrogen sources in its environment. In conditions of derepression (under poor nitrogen conditions, upon rapamycin treatment, or when glutamine production is inhibited), two transcriptional activators of the GATA family are recruited to NCR‐sensitive promoters and activate transcription of NCR‐sensitive genes. Earlier observations have involved the Spt‐Ada‐Gcn5 acetyltransferase (SAGA) chromatin remodeling complex in these transcriptional regulations. In this report, we provide an illustration of the varying NCR‐sensitive responses and question whether differing SAGA recruitment could explain this diversity of responses.

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supporting

confidence: 90%

Differing SAGA module requirements for NCR‐sensitive gene transcription in yeast

Georis

Ronsmans

Vierendeels

et al. 2023

Yeast

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“…Ada1 also functions in delimiting the boundaries of gene silencing in chromatin and this function was linked to proteasome processes ( Kamata et al, 2016 ). In addition, Ada1 is also involved in repairing radiation damage ( Game et al, 2005 ), regulation of gene expression in the yeast metabolic cycle ( Sánchez-Gaya et al, 2018 ), and nuclear localization of Ubp8 subunit ( Nuño-Cabanes et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

The Verticillium dahliae Spt-Ada-Gcn5 Acetyltransferase Complex Subunit Ada1 Is Essential for Conidia and Microsclerotia Production and Contributes to Virulence

Geng

Wang

et al. 2022

Front. Microbiol.

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Verticillium dahliae is a destructive soil-borne pathogen of many economically important dicots. The genetics of pathogenesis in V. dahliae has been extensively studied. Spt-Ada-Gcn5 acetyltransferase complex (SAGA) is an ATP-independent multifunctional chromatin remodeling complex that contributes to diverse transcriptional regulatory functions. As members of the core module in the SAGA complex in Saccharomyces cerevisiae, Ada1, together with Spt7 and Spt20, play an important role in maintaining the integrity of the complex. In this study, we identified homologs of the SAGA complex in V. dahliae and found that deletion of the Ada1 subunit (VdAda1) causes severe defects in the formation of conidia and microsclerotia, and in melanin biosynthesis and virulence. The effect of VdAda1 on histone acetylation in V. dahliae was confirmed by western blot analysis. The deletion of VdAda1 resulted in genome-wide alteration of the V. dahliae transcriptome, including genes encoding transcription factors and secreted proteins, suggesting its prominent role in the regulation of transcription and virulence. Overall, we demonstrated that VdAda1, a member of the SAGA complex, modulates multiple physiological processes by regulating global gene expression that impinge on virulence and survival in V. dahliae.

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SAGA Complex Subunit Hfi1 Is Important in the Stress Response and Pathogenesis of Cryptococcus neoformans

Yu,

Stephenson,

Villamor

et al. 2023

JoF

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The Spt-Ada-Gcn Acetyltransferase (SAGA) complex is a highly conserved co-activator found across eukaryotes. It is composed of a number of modules which can vary between species, but all contain the core module. Hfi1 (known as TADA1 in Homo sapiens) is one of the proteins that forms the core module, and has been shown to play an important role in maintaining complex structural integrity in both brewer’s yeast and humans. In this study we successfully identified the gene encoding this protein in the important fungal pathogen, Cryptococcus neoformans, and named it HFI1. The hfi1Δ mutant is highly pleiotropic in vitro, influencing phenotypes, ranging from temperature sensitivity and melanin production to caffeine resistance and titan cell morphogenesis. In the absence of Hfi1, the transcription of several other SAGA genes is impacted, as is the acetylation and deubiquination of several histone residues. Importantly, loss of the gene significantly impacts virulence in a murine inhalation model of cryptococcosis. In summary, we have established that Hfi1 modulates multiple pathways that directly affect virulence and survival in C. neoformans, and provided deeper insight into the importance of the non-enzymatic components of the SAGA complex.

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SAGA–CORE subunit Spt7 is required for correct Ubp8 localization, chromatin association and deubiquitinase activity

Cited by 3 publications

References 50 publications

Differing SAGA module requirements for NCR‐sensitive gene transcription in yeast

Differing SAGA module requirements for NCR‐sensitive gene transcription in yeast

The Verticillium dahliae Spt-Ada-Gcn5 Acetyltransferase Complex Subunit Ada1 Is Essential for Conidia and Microsclerotia Production and Contributes to Virulence

SAGA Complex Subunit Hfi1 Is Important in the Stress Response and Pathogenesis of Cryptococcus neoformans

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