Saffron suppresses oxidative stress in DMBA-induced skin carcinoma: A histopathological study

Das, Ila; Das, Sukta; Saha, Tapas Kumar

doi:10.1016/j.acthis.2009.02.003

Cited by 107 publications

(66 citation statements)

References 35 publications

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“…Saffron is a derived plant product from the dried stigma of the Crocus sativus flower (family, Iridaceae), which has been used for several hundred years in the treatment of cardiovascular diseases, inflammatory diseases and several other tumors (10,11). Extracts of saffron contain multiple biologically active compounds, including crocin, crocetin, saffron bitter element, saffron aldehyde, vitamins and flavonoids.…”

Section: Introductionmentioning

confidence: 99%

Crocetin induces apoptosis of BGC-823 human gastric cancer cells

Wang

et al. 2013

Molecular Medicine Reports

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Abstract. Gastric cancer (GC) is one of the most common types of gastrointestinal tumors worldwide, and the side effects of chemotherapeutic drugs and the resistance to chemotherapy remain problematic in its clinical treatment. Therefore, safe and effective novel agents are urgently required. The purpose of the present study was to investigate the crocetin-sensitive treatment of GC and its possible mechanisms. BGC-823 human GC cells were treated with crocetin. The effects of crocetin on the viability of the cells were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Hoechst 33258 dyeing and Rh123 staining were used to detect cell apoptosis. The BGC-823 cells were subjected to western blotting analysis for detection of cytochrome c and cleaved caspase-3 protein expression. Crocetin inhibited the proliferation of the GC cell line in a dose-and time-dependent manner. Apoptotic BGC-823 cells induced by crocetin were stained by Hoechst 33258 and observed under a light microscope for cell membrane staining of dense nuclei, nuclear pyknosis, fragmentation, chromatin condensation and highlighted nuclear membrane staining. This revealed a decline in the mitochondrial membrane potential of the BGC-823 cells. Crocetin also induced caspase-3 activation and cytochrome c translocation into the cytosol from the mitochondria. The results of this study indicate that crocetin induces the apoptosis of BGC-823 cells, and may be used as an effective agent in the treatment of GC.

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Section: Introductionmentioning

confidence: 99%

Crocetin induces apoptosis of BGC-823 human gastric cancer cells

Wang

et al. 2013

Molecular Medicine Reports

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“…The DMBA initiates skin tumor formation through the generation ROS that are responsible for genetic materials damage (Das et al, 2010). Therefore, the chemoprevention activity of MEMM could be attributed to the extract's antioxidant activity, which, in turn, could be associated with the extract's phytochemical constituents (e.g.…”

Section: Discussionmentioning

confidence: 99%

Chemopreventive activity of methanol extract of <i>Melastoma malabathricum</i> leaves in DMBA-induced mouse skin carcinogenesis

Kooi¹,

Ling²,

Rodzi³

et al. 2014

Afr. J. Trad. Compl. Alt. Med.

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Background: Melastoma malabathricum L. Smith (family Melastomaceae) is a shrub that has been used by the Malay practitioners of traditional medicine to treat various types of ailments. The present study aimed to determine the chemopreventive activity of methanol extract of M. malabathricum leaves (MEMM) using the standard 7,12-dimethylbenz(α)anthracene (DMBA)/croton oil-induced mouse skin carcinogenesis model. Materials and Methods:In the initiation phase, the mice received a single dose of 100µl/100 µg DMBA (group I-V) or 100µl acetone (group VI) topically on the dorsal shaved skin area followed by the promotion phase involving treatment with the respective test solutions (100 μl of acetone, 10 mg/kg curcumin or MEMM (30, 100 and 300mg/kg)) for 30 min followed by the topical application of tumour promoter (100µl croton oil). Tumors were examined weekly and the experiment lasted for 15 weeks. Results: MEMM and curcumin significantly (p<0.05) reduced the tumour burden, tumour incidence and tumour volume, which were further supported by the histopathological findings. Conclusion: MEMM demonstrated chemoprevention possibly via its antioxidant and anti-inflammatory activities, and the action of flavonoids like quercitrin.

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“…Aqueous saffron preparations have been reported to inhibit chemically induced skin carcinogenesis (Das et al, 2004). Both changes in carcinogen bio activation and tumour proliferation appear to occur (Das et al, 2010). Curcumin (diferuloylmethane) is a polyphenol derived from the Curcuma longa plant, commonly known as turmeric.…”

Section: * Corresponding Authormentioning

confidence: 99%

Molecular Docking Studies of Phytoconstituents Identified in Crocus sativus, Curcuma longa, Cassia occidentalis and Moringa oleifera on Thymidylate Synthase and ndash; An Enzyme Target for Anti-Cancer Activity

Heble¹,

Mavillapalli²,

Selvaraj³

et al. 2016

J App Pharm Sci

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The aim and objective of the present study is to perform in-silico docking analysis of the major active constituents identified in four Indian medicinal plants namely Crocus sativus, Curcuma longa, Cassia occidentalis and Moringa oleifera for anti-cancer activity. In-silico docking analysis was performed by using Molegro Virtual Docker (MVD). The parameter used for the docking analysis are MolDock score, Rerank score and H-Bond interactions (binding energy). The target for anti-cancer activity is thymidylate synthase. The XRay crystal co-ordinate of thymidylate synthase (PDB ID-1HVY) was retrieved from protein data bank in .pdb format. The phytoconstituents of four medicinal plants were retrieved from PubChem compound database in .mol format. The standard drugs Ralitrexed, 5-Fluorouracil and Vinblastine were obtained from the drug bank in .mol format for comparison. The comparative anti-cancer activity of the phytoconstituents of four medicinal plants are analysed by docking score and binding energy. It was analysed from the parameters of docking that the phytoconstituents from Crocus sativum showed better anti-cancer activity compared to that of the standard drugs.

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Saffron suppresses oxidative stress in DMBA-induced skin carcinoma: A histopathological study

Cited by 107 publications

References 35 publications

Crocetin induces apoptosis of BGC-823 human gastric cancer cells

Crocetin induces apoptosis of BGC-823 human gastric cancer cells

Chemopreventive activity of methanol extract of <i>Melastoma malabathricum</i> leaves in DMBA-induced mouse skin carcinogenesis

Molecular Docking Studies of Phytoconstituents Identified in Crocus sativus, Curcuma longa, Cassia occidentalis and Moringa oleifera on Thymidylate Synthase and ndash; An Enzyme Target for Anti-Cancer Activity

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