Safety of Technique and Procedure of Stromal Vascular Fraction Therapy: From Liposuction to Cell Administration

Abstract

<jats:p><jats:italic>Background</jats:italic>. Stromal vascular fraction (SVF) therapy has been performed over the past six years to treat 421 patients by our group in five clinical centers. Autologous SVF, which is a substance containing stem cells, was isolated from lipoaspirate, mixed with platelet-rich plasma (PRP), and administered to patients with degenerative diseases, autoimmune diseases, trauma, aging, and other diseases with unknown etiology. This study aimed to determine the safety of SVF and PRP that were given through infusion, spinal, and intra-articular injection. <jats:italic>Methods</jats:italic>. The lipoaspirate was treated with a tissue-dissociating enzyme, and then, through centrifugation, SVF was isolated. In addition, blood was drawn from each patient, and PRP was isolated. Autologous PRP and SVF were administered to all subjects by intravenous (IV) injection. A minority group within the population received an additional spinal or intra-articular injection. The type of intervention was determined by each disease evaluation. The cell doses and adverse events for each patient were documented and analyzed. <jats:italic>Results</jats:italic>. Cell dose that was considered to be safe was less than 10 billion SVF cells in 250 cc of normal saline, for IV injection, and less than 1 billion SVF, for intra-articular and spinal injection. Adverse events were not severe and were treated successfully. Any observed adverse events were identified as a result of spinal or intra-articular injections and were not related to SVF or PRP. <jats:italic>Conclusions</jats:italic>. Our results showed that administration of high dose of SVF until 10 billion cells in a majority of 421 patients through infusion, spinal, and intra-articular injection was feasible without causing major adverse events and should be further investigated in well-designed phase I-II clinical trial to address the safety and efficacy of therapy.</jats:p>

Evaluating the Safety of Intravenous Delivery of Autologous Activated Platelet-rich Plasma

“…PRP has also been reported to be beneficial for ischemic stroke, osteoarthritis, and chronic ulcers. There have been no previous reports regarding the adverse event of PRP use in those cases or its combination with stromal vascular fraction (11,12,30,31,32,33). Our study showed that the intravenous administration of aaPRP in patients with various pathological conditions did not cause any adverse event.…”

The Effect of Intravenous Autologous Activated Platelet-Rich Plasma Therapy on “Profibrotic Cytokine” IL-1β Levels in Severe and Critical COVID-19 Patients: A Preliminary Study

“…Activated autologous platelet-rich plasma (aaPRP) is a prospective therapy to be performed in COVID-19 patients to combat such systemic inflammation. Apart from its accessibility, it also possesses anti-inflammatory properties through cytokines such as interleukin-1 receptor antagonist (IL-1RA) and a known safety profile [11][12][13][14]. Similarly, anakinra, a recombinant IL1-RA, showed promising results in a cohort consisting of patients with severe forms COVID-19 [15].…”

Efficacy of Adipose-Derived Stem Cells as a Potential Cellular Therapy of Alzheimer’s Disease: A Comprehensive Systematic Review

“…Besides being minimally invasive to extract, ADSCs are abundant in source because fat tissue can be found almost everywhere in the body. Another rationale for utilizing ADSCs as cellular therapy is their multipotency and autologous usage, which allows to avoid immunological rejection and tumorigenesis (15)(16)(17).…”