Safety from infection in the microbiology laboratory

doi:10.1016/s0163-4453(80)91059-2

Journal of Infection

1980

DOI: 10.1016/s0163-4453(80)91059-2

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Safety from infection in the microbiology laboratory

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1987

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Standardization of a Chemiluminescence Method for the Measurement of Meningococcal Opsonins Using Ethanol Fixed Meningococci

Halstensen

Haneberg

1987

Acta Pathologica Microbiologica Scandinavica Series C: Immunolo

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Halstensen. A. & Haneberg. B. Standardization of a chemiluminescence method for the measurement of meningococcal opsonins using ethanol fixed meningococci. Acta path. microbiol. immunol. scand. Sect. C. YF: 155-160. 1987.A chemiluminescence (CL) method using polymorphonuclear leukocytes (PMNLs) and an automatic photoluminometer was used to measure serum opsonins to viable and inactivated group B meningococci. Continuous mixing at 37 'C both during opsonization and phagocytosis was essential for optimal CL responses. The CL response increased rapidly during an opsonization time up to 7.5 min. and with PMNL and bacteria concentrations up to 37.5 x lo5 and 3.8 x lo7 cells/rnl, respectively. Opsonized ethanol fixed meningococci gave CL responses similar to those of viable meningococci. but had a better reproducibility. Using the ethanol fixed bacteria, the variation of PMNLs from different donors, the dayto-day variation. and the coefficient of variation of the CL responses, were all less than 10%. The opsonic activity of convalescent sera from 10 patients with meningococcal disease was markedly higher than that of sera obtained during the acute phase of the disease. Thus, this standardized CL assay using ethanol fixed bacteria is a highly reproducible and sensitive method for measuring serum opsonins to meningococci.

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Standardization of a Chemiluminescence Method for the Measurement of Meningococcal Opsonins Using Ethanol Fixed Meningococci

Halstensen

Haneberg

1987

Acta Pathologica Microbiologica Scandinavica Series C: Immunolo

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Safety from infection in the microbiology laboratory

Cited by 1 publication

References 8 publications

Standardization of a Chemiluminescence Method for the Measurement of Meningococcal Opsonins Using Ethanol Fixed Meningococci

Standardization of a Chemiluminescence Method for the Measurement of Meningococcal Opsonins Using Ethanol Fixed Meningococci

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