Safety Concerns Using Cell-Based In Vitro Methods for Toxicity Assessment

Shingatgeri, Vyas

doi:10.1016/b978-0-12-804667-8.00010-9

Cited by 3 publications

(1 citation statement)

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“…Cell cultures represents an immense value in the investigation of cellular and functional aspects of disease processes for improved therapeutic interventions. [ 8 ] The most commonly utilized model systems are based on conventional 2D monolayer cultures exposed to exogenous profibrotic stimuli, commonly transforming growth factor-β (TGF-β). [ 9 ] However, customarily used cell-based models frequently fail to give predictable and reliable data for in vivo responses.…”

Section: Introductionmentioning

confidence: 99%

Engineering Advanced In Vitro Models of Systemic Sclerosis for Drug Discovery and Development

et al. 2021

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Systemic sclerosis (SSc) is a complex multisystem disease with the highest case‐specific mortality among all autoimmune rheumatic diseases, yet without any available curative therapy. Therefore, the development of novel therapeutic antifibrotic strategies that effectively decrease skin and organ fibrosis is needed. Existing animal models are cost‐intensive, laborious and do not recapitulate the full spectrum of the disease and thus commonly fail to predict human efficacy. Advanced in vitro models, which closely mimic critical aspects of the pathology, have emerged as valuable platforms to investigate novel pharmaceutical therapies for the treatment of SSc. This review focuses on recent advancements in the development of SSc in vitro models, sheds light onto biological (e.g., growth factors, cytokines, coculture systems), biochemical (e.g., hypoxia, reactive oxygen species) and biophysical (e.g., stiffness, topography, dimensionality) cues that have been utilized for the in vitro recapitulation of the SSc microenvironment, and highlights future perspectives for effective drug discovery and validation.

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Section: Introductionmentioning

confidence: 99%