Safety assessment of potential probiotic lactic acid bacterial strains Lactobacillus rhamnosus HN001, Lb. acidophilus HN017, and Bifidobacterium lactis HN019 in BALB/c mice

New probiotic strain Lactobacillus fermentum AD1 and its effect in Japanese quail

“…Concerning the haematology, no significant differences were observed after L. fermentum AD1 application. These results agree with the findings of Zhou et al (2000), who tested potential probiotic lactic acid bacterial strains L. rhamnosus, L. acidophilus and Bifidobacterium lactis in mice. The enzyme glutathione peroxidase, a component of the antioxidative defence system, was not affected.…”

Safety Assessment of Lactobacillus salivarius REN, a Probiotic Strain Isolated from Centenarian Feces

“…No noticeable activity or behavioral changes were observed in mice during the experimental protocol, and no illness or death occurred. Oral administration of different doses of L. salivarius REN caused no significant difference in BW, food intake, and tissue weight among the groups throughout the experiment (Table 1), which are similar with previous reports (Zhou et al, 2000;Lara-Villoslada et al, 2007a). These results demonstrate that L. salivarius REN does not exhibit gross nutrient metabolism (Swendseid, 1987), which usually apparent before any clinical symptoms (Zhou et al, 2000).…”

“…Oral administration of different doses of L. salivarius REN caused no significant difference in BW, food intake, and tissue weight among the groups throughout the experiment (Table 1), which are similar with previous reports (Zhou et al, 2000;Lara-Villoslada et al, 2007a). These results demonstrate that L. salivarius REN does not exhibit gross nutrient metabolism (Swendseid, 1987), which usually apparent before any clinical symptoms (Zhou et al, 2000). The changes of serum biochemical parameters might indicate responsive organs were affected or even damaged (Swendseid, 1987).The current study showed that oral administration of L. salivarius REN for 28 days had no adverse effects on the hematology and blood biochemistry (Table 2).…”

“…Blood samples were collected into EDTA-containing tubes by cardiac puncture in sterile conditions, and serum oral toxicity on the animals' health status, growth and development, even in high dose groups. In order to evaluate the adverse sub-clinical effects of L. salivarius REN, hematological and general blood biochemical parameters were monitored (Zhou et al, 2000). Hematological parameters are usually used to represent the deleterious effects of test substance on the blood (Lee et al, 2010), when the composition of blood cells change, it might indicate the pathological changes in bone marrow or the reaction of immune cells (Yakubu et al, 2007).…”

Phenotypic Safety Assessment and Molecular Characterization of Enterococcus feacium MBTU-P1F1 (KF745071) from Infant feces

“…All these observations give information on the safety of viable administration of the test strain Enterococcus faecium MBTU-P1F1 as a probiotic. Assessment of pathogenicity is one of the important components of probiotic safety studies, the indicators for which include splenomegaly and hepatomegaly (Zhou et al, 2000). Figure 6a, 6b, 6c and 6d represent the photomicrographs of the intestinal sections of the test group mice and the control group mice.…”