Safety assessment of Mpp75Aa1.1, a new ETX_MTX2 protein from Brevibacillus laterosporus that controls western corn rootworm

Wang, Cunxi; Bean, Gregory J.; Chen, Chun Ju; Kessenich, Colton R.; Peng, Jiexin; Visconti, Nicolo; Milligan, Jason; Moore, Robert G.; Tan, Jian; Edrington, Thomas; Li, Bin; Giddings, Kara S.; Bowen, David; Luo, Jinhua; Ciche, Todd A.; Moar, William J.

doi:10.1371/journal.pone.0274204

Cited by 3 publications

(1 citation statement)

References 71 publications

(152 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Iurlina & Fritz, 2005 ; Panda et al., 2014 ; Sarkar et al., 2002 ). B. laterosporus is also used as a commercial probiotic, human dietary supplement and feed additive (Liu et al., 2023 ; Liu et al., 2024 ; Wang et al., 2022 ). However, the amount of Mpp75Aa1.1 protein consumption by humans and animals through exposure to B. laterosporus has not been established.…”

Section: Assessmentmentioning

confidence: 99%

Assessment of genetically modified maize MON 95275 (application GMFF‐2022‐5890)

Mullins,

Bresson,

Dalmay

et al. 2024

EFS2

View full text Add to dashboard Cite

Genetically modified maize MON 95275 was developed to confer protection to certain coleopteran species. These properties were achieved by introducing the mpp75Aa1.1, vpb4Da2 and DvSnf7 expression cassettes. The molecular characterisation data and bioinformatic analyses reveal similarity to known toxins, which was further assessed. None of the identified differences in the agronomic/phenotypic and compositional characteristics tested between maize MON 95275 and its conventional counterpart needs further assessment. The GMO Panel does not identify safety concerns regarding the toxicity and allergenicity of the Mpp75Aa1.1 and Vpb4Da2 proteins and the DvSnf7 dsRNA and derived siRNAs as expressed in maize MON 95275 and finds no evidence that the genetic modification would change the overall allergenicity of maize MON 95275. In the context of this application, the consumption of food and feed from maize MON 95275 does not represent a nutritional concern in humans and animals. The GMO Panel concludes that maize MON 95275 is as safe as the conventional counterpart and non‐GM maize varieties tested, and no post‐market monitoring of food/feed is considered necessary. In the case of accidental release of maize MON 95275 material into the environment, this would not raise environmental safety concerns. The post‐market environmental monitoring plan and reporting intervals are in line with the intended uses of maize MON 95275. The GMO Panel concludes that maize MON 95275 is as safe as its conventional counterpart and the tested non‐GM maize varieties with respect to potential effects on human and animal health and the environment.

show abstract

Section: Assessmentmentioning

confidence: 99%

Assessment of genetically modified maize MON 95275 (application GMFF‐2022‐5890)

Mullins,

Bresson,

Dalmay

et al. 2024

EFS2

View full text Add to dashboard Cite

show abstract

Cotton plants overexpressing the Bacillus thuringiensis Cry23Aa and Cry37Aa binary-like toxins exhibit high resistance to the cotton boll weevil (Anthonomus grandis)

Ribeiro,

Martins-de-Sa,

Macedo

et al. 2024

Plant Science

View full text Add to dashboard Cite

Production and characterization of homologous protoporphyrinogen IX oxidase (PPO) proteins: Evidence that small N-terminal amino acid changes do not impact protein function

Wang,

Zheng,

Est

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

Transgenic soybean, cotton, and maize tolerant to protoporphyrinogen IX oxidase (PPO)-inhibiting herbicides have been developed by introduction of a bacterial-derived PPO targeted into the chloroplast. PPO is a membrane-associated protein with an intrinsic tendency for aggregation, making expression, purification, and formulation at high concentrations difficult. In this study, transgenic PPO expressed in three crops was demonstrated to exhibit up to a 13 amino acid sequence difference in the N-terminus due to differential processing of the chloroplast transit peptide (CTP). Five PPO protein variants were produced in and purified from E. coli, each displaying equivalent immunoreactivity and functional activity, with values ranging from 193 to 266 nmol min-1 mg-1. Inclusion of an N-terminal 6xHis-tag or differential processing of the CTP peptide does not impact PPO functional activity. Additionally, structural modeling by Alphafold, ESMfold, and Openfold indicates that these short N-terminal extensions are disordered and predicted to not interfere with the mature PPO structure. These results support the view that safety studies on PPO from various crops can be performed from a single representative variant. Herein, we report a novel and robust method for large-scale production of PPO, enabling rapid production of more than 200 g of highly active PPO protein at 99% purity and low endotoxin contamination. We also present a formulation that allows for concentration of active PPO to > 75 mg/mL in a buffer suitable for mammalian toxicity studies.

show abstract

Safety assessment of Mpp75Aa1.1, a new ETX_MTX2 protein from Brevibacillus laterosporus that controls western corn rootworm

Cited by 3 publications

References 71 publications

Assessment of genetically modified maize MON 95275 (application GMFF‐2022‐5890)

Assessment of genetically modified maize MON 95275 (application GMFF‐2022‐5890)

Cotton plants overexpressing the Bacillus thuringiensis Cry23Aa and Cry37Aa binary-like toxins exhibit high resistance to the cotton boll weevil (Anthonomus grandis)

Production and characterization of homologous protoporphyrinogen IX oxidase (PPO) proteins: Evidence that small N-terminal amino acid changes do not impact protein function

Contact Info

Product

Resources

About