Safety and tolerability of silk fibroin hydrogels implanted into the mouse brain

Fernández-García, Laura; Marí-Buyé, Núria; Barios, Juan A.; Madurga, Rodrigo; Elices, M.; Pérez‐Rigueiro, José; Ramos, Milagros; Guinea, Gustavo V.; González‐Nieto, Daniel

doi:10.1016/j.actbio.2016.09.003

Cited by 89 publications

(106 citation statements)

References 61 publications

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“…Formation of β‐amyloid structures is a concern because amyloid beta fibrils are a hallmark of Alzheimer's disease. [61c,93] Preliminary studies in mice injected with self‐assembling silk fibroin hydrogels into the caudate putamen (striatum) showed no decline in cognitive function or animal behavior over the 6 week study period …”

Section: Silk For Tissue Engineering and Drug Delivery—expectations mentioning

confidence: 99%

The Biomedical Use of Silk: Past, Present, Future

Holland

Numata

Rnjak‐Kovacina

et al. 2018

Adv Healthcare Materials

587

509

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Humans have long appreciated silk for its lustrous appeal and remarkable physical properties, yet as the mysteries of silk are unraveled, it becomes clear that this outstanding biopolymer is more than a high‐tech fiber. This progress report provides a critical but detailed insight into the biomedical use of silk. This journey begins with a historical perspective of silk and its uses, including the long‐standing desire to reverse engineer silk. Selected silk structure–function relationships are then examined to appreciate past and current silk challenges. From this, biocompatibility and biodegradation are reviewed with a specific focus of silk performance in humans. The current clinical uses of silk (e.g., sutures, surgical meshes, and fabrics) are discussed, as well as clinical trials (e.g., wound healing, tissue engineering) and emerging biomedical applications of silk across selected formats, such as silk solution, films, scaffolds, electrospun materials, hydrogels, and particles. The journey finishes with a look at the roadmap of next‐generation recombinant silks, especially the development pipeline of this new industry for clinical use.

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Section: Silk For Tissue Engineering and Drug Delivery—expectations mentioning

confidence: 99%

The Biomedical Use of Silk: Past, Present, Future

Holland

Numata

Rnjak‐Kovacina

et al. 2018

Adv Healthcare Materials

587

509

View full text Add to dashboard Cite

show abstract

“…However, in the case of fields like ophthalmology, volumes less than 100 µL may be used . For small animal studies, which are common preclinical stages in the biomaterial translational pipeline, volumes may be 5 µL or less . In these cases, one might expect a higher effort score may be allowable, for only a short duration.…”

Section: Discussionmentioning

confidence: 99%

Filling the Gap: A Correlation between Objective and Subjective Measures of Injectability

Robinson

Hughes

Bose

et al. 2020

Adv Healthcare Materials

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Various injectable biomaterials are developed for the minimally invasive delivery of therapeutics. Typically, a mechanical tester is used to ascertain the force required to inject these biomaterials through a given syringe‐needle system. However, currently there is no method to correlate the force measured in the laboratory to the perceived effort required to perform that injection by the end user. In this article, the injection force (F) for a variety of biomaterials, displaying a range of rheological properties, is compared with the effort scores from a 50 person panel study. The maximum injection force measured at crosshead speed 1 mm s−1 is a good proxy for injection effort, with an R2 of 0.89. This correlation leads to the following conclusions: participants can easily inject 5 mL of substance for F < 12 N; considerable effort is required to inject 5 mL for 12 N < F < 38 N; great effort is required and <5 mL can be injected for 38 N < F < 64 N; and materials are entirely non‐injectable for F > 64 N. These values may be used by developers of injectable biomaterials to make decisions about formulations and needle sizes early in the translational process.

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“…Tang et al have also demonstrated that silk fibroin fibers promote the survival and growth of rat hippocampal neurons and administration of silk fibroin extract into the cell culture medium exerts no cytotoxic effects on the hippocampal neurons phenotype or functions (Tang et al, 2009). The tolerability of the implantation of silk fibroin hydrogels into the mice striatum has also been reported without inducing cell death or cognitive and sensorimotor deficits (Fernández-García et al, 2016). It has been demonstrated that electrospun silk fibroin nanofibers promote the growth and expansion of the Schwann cells, suggesting that silk fibroin can promote the regeneration of axons following axotomy and nerve-crush traumas (Hu et al, 2012).…”

Section: Neuroregenerationmentioning

confidence: 98%

Identification and applications of neuroactive silk proteins: a narrative review

Mohammadi¹,

Sadigh‐Eteghad²,

Torbati³

et al. 2019

J Appl Biomed

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In traditional medicine, natural silk is regarded as a cognitive enhancer and a cure for ameliorating the symptoms of heart disease, atherosclerosis, and metabolic disorders. In this review, general characteristics of both silk proteins, fibroin and sericin, extracted from silkworm Bombyx mori and their potential use in the neuronal disorders was discussed. Evidence shows that silk proteins exhibit neuroprotective effects in models of neurotoxicity. The antioxidant, neuroprotective, and acetylcholinesterase inhibitory mechanisms of silk proteins could prove promising in the treatment of neurodegenerative diseases. Owing to their excellent neurocompatibility and physicochemical properties, silk proteins have been used as scaffolds and drug delivery materials in the neuronal tissue engineering. These data support the potential of silk proteins as an effective complementary agent for central and peripheral neurological disorders.

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Safety and tolerability of silk fibroin hydrogels implanted into the mouse brain

Cited by 89 publications

References 61 publications

The Biomedical Use of Silk: Past, Present, Future

The Biomedical Use of Silk: Past, Present, Future

Filling the Gap: A Correlation between Objective and Subjective Measures of Injectability

Identification and applications of neuroactive silk proteins: a narrative review

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