Safety and possible outcome assessment of autologous Schwann cell and bone marrow mesenchymal stromal cell co-transplantation for treatment of patients with chronic spinal cord injury

Oraee‐Yazdani, Saeed; Hafizi, Maryam; Zali, Alireza; Atashi, Amir; Ashrafi, Farzad; Seddighi, Amirsaeed; Soleimani, Masoud

doi:10.1016/j.jcyt.2013.03.012

Cited by 60 publications

(65 citation statements)

References 36 publications

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“…When used as grafts, these cells have the potential for clinical autologous transplantation, without the risk of severe immune rejection. Clinical investigations have indicated that the transplantation of autologous MSCs is feasible and safe [47][48][49][50] . PLGA has been widely used as an artificial catheter, drug controlled-release carrier, and tissue engineering scaffold material for its mechanical properties and biodegradation.…”

Section: Discussionmentioning

confidence: 99%

Multichannel polymer scaffold seeded with activated Schwann cells and bone mesenchymal stem cells improves axonal regeneration and functional recovery after rat spinal cord injury

Yang

Zhang

et al. 2017

Acta Pharmacol Sin

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The adult mammalian CNS has a limited capacity to regenerate after traumatic injury. In this study, a combinatorial strategy to promote axonal regeneration and functional recovery after spinal cord injury (SCI) was evaluated in adult rats. The rats were subjected to a complete transection in the thoracic spinal cord, and multichannel scaffolds seeded with activated Schwann cells (ASCs) and/or rat bone marrow-derived mesenchymal stem cells (MSCs) were acutely grafted into the 3-mm-wide transection gap. At 4 weeks posttransplantation and thereafter, the rats receiving scaffolds seeded with ASCs and MSCs exhibited significant recovery of nerve function as shown by the Basso, Beattie and Bresnahan (BBB) score and electrophysiological test results. Immunohistochemical analyses at 4 and 8 weeks after transplantation revealed that the implanted MSCs at the lesion/graft site survived and differentiated into neuron-like cells and co-localized with host neurons. Robust bundles of regenerated fibers were identified in the lesion/graft site in the ASC and MSC co-transplantation rats, and neurofilament 200 (NF) staining confirmed that these fibers were axons. Furthermore, myelin basic protein (MBP)-positive myelin sheaths were also identified at the lesion/graft site and confirmed via electron microscopy. In addition to expressing mature neuronal markers, sparse MSC-derived neuron-like cells expressed choline acetyltransferase (ChAT) at the injury site of the ASC and MSC co-transplantation rats. These findings suggest that co-transplantation of ASCs and MSCs in a multichannel polymer scaffold may represent a novel combinatorial strategy for the treatment of spinal cord injury.

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Section: Discussionmentioning

confidence: 99%

Multichannel polymer scaffold seeded with activated Schwann cells and bone mesenchymal stem cells improves axonal regeneration and functional recovery after rat spinal cord injury

Yang

Zhang

et al. 2017

Acta Pharmacol Sin

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“…5 Briefly, the sural nerve of the patient that was collected from the calf region was sliced into 1-to 2-mm pieces and treated with collagenase (1.4 U ml − 1 ; Sigma, St. Louis, MO, USA) and Dispase (2.4 U ml − 1 ; Sigma). At first, the cells were incubated in DMEM/F12, not including fetal bovine serum (FBS, Gibco), for 5 days.…”

Section: Cell Isolation and Transplantationmentioning

confidence: 99%

“…The isolated cells were determined with S100 immunocytological staining and double checked with P75 immunocytological staining using the same protocol used in our previous study. 5 Bone marrow blood (100 ml) was aspirated from the iliac bone for collection of bone marrow stem cells according to the protocol that we used in our previous study. 5 In brief, the samples went through a density gradient by Ficoll (1.077 g l − 1 , Sigma) at a ratio of 1:3.…”

Section: Cell Isolation and Transplantationmentioning

confidence: 99%

“…5 Bone marrow blood (100 ml) was aspirated from the iliac bone for collection of bone marrow stem cells according to the protocol that we used in our previous study. 5 In brief, the samples went through a density gradient by Ficoll (1.077 g l − 1 , Sigma) at a ratio of 1:3. After centrifugation, the mononuclear cell layer was recovered from the gradient interface.…”

Section: Cell Isolation and Transplantationmentioning

confidence: 99%

“…4 In our previous study, we assessed the safety and possible outcome of autologous SC and bone marrow MSC co-transplantation for the treatment of patients with chronic spinal cord injury through direct transplantation of this combination into the injured area. 5 In many studies, the cells have been injected into the lesion directly through a needle. In addition to the procedure, this approach is potentially harmful to the parenchyma; direct injection into the spine lesion with a needle is clinically unrealistic because the lesion is widely spread or multifocal.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Co-transplantation of autologous bone marrow mesenchymal stem cells and Schwann cells through cerebral spinal fluid for the treatment of patients with chronic spinal cord injury: safety and possible outcome

et al. 2015

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Advances in Skin Regeneration: Application of Electrospun Scaffolds

Norouzi

Boroujeni

Omidvarkordshouli

et al. 2015

Adv Healthcare Materials

237

182

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The paucity of cellular and molecular signals essential for normal wound healing makes severe dermatological ulcers stubborn to heal. The novel strategies of skin regenerative treatments are focused on the development of biologically responsive scaffolds accompanied by cells and multiple biomolecules resembling structural and biochemical cues of the natural extracellular matrix (ECM). Electrospun nanofibrous scaffolds provide similar architecture to the ECM leading to enhancement of cell adhesion, proliferation, migration and neo tissue formation. This Review surveys the application of biocompatible natural, synthetic and composite polymers to fabricate electrospun scaffolds as skin substitutes and wound dressings. Furthermore, the application of biomolecules and therapeutic agents in the nanofibrous scaffolds viz growth factors, genes, antibiotics, silver nanoparticles, and natural medicines with the aim of ameliorating cellular behavior, wound healing, and skin regeneration are discussed.

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Safety and possible outcome assessment of autologous Schwann cell and bone marrow mesenchymal stromal cell co-transplantation for treatment of patients with chronic spinal cord injury

Cited by 60 publications

References 36 publications

Multichannel polymer scaffold seeded with activated Schwann cells and bone mesenchymal stem cells improves axonal regeneration and functional recovery after rat spinal cord injury

Multichannel polymer scaffold seeded with activated Schwann cells and bone mesenchymal stem cells improves axonal regeneration and functional recovery after rat spinal cord injury

Co-transplantation of autologous bone marrow mesenchymal stem cells and Schwann cells through cerebral spinal fluid for the treatment of patients with chronic spinal cord injury: safety and possible outcome

Advances in Skin Regeneration: Application of Electrospun Scaffolds

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