Safety and efficacy profile of a phytase produced by fermentation and used as a feed additive

Thorsen, Michael; Nielsen, L.; Zhai, Huan-Chen; Zhang, Qian; Wulf-Andersen, Linda; Skov, L.K.

doi:10.1016/j.heliyon.2021.e07237

Cited by 12 publications

(3 citation statements)

References 36 publications

(31 reference statements)

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“…In addition, the depression of the phytase enzyme was influenced by the number of bacterial colonies. A high number of bacterial colonies will produce more phytase enzyme, and conversely, a decrease in phytase enzyme would portend a depression in bacterial colony number (Thorsen et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

Pre-treatments of Mirasolia diversifolia using Lactobacillus bulgaricus at different dosages and fermentation times: Phytic acid concentration, enzyme activity, and fermentation characteristics

Pazla,

Putri,

Jamarun

et al. 2024

SA J. An. Sci.

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The present study aimed to determine the optimal dosage and fermentation time of Mirasolia diversifolia (leaves and stems) using Lactobacillus bulgaricus bacteria by observing the reduction in phytic acid, the activity of enzymes, pH of fermentation, number of bacterial colonies, and tannin concentration. An experiment was conducted using a completely randomized design (CRD) and 2×3 factorial design with three replications. The treatment factors were Factor A (L. bulgaricus dosages): A1 = 2% (g/v), A2 = 3% (g/v); and Factor B (fermentation times): B1 = 1 d, B2 = 3 d, B3 = 5 d. The dosage of L. bulgaricus and fermentation time had an interaction on phytic acid and its degradation, enzyme activities, pH fermentation, and the number of bacterial colonies. Tannin concentration was not affected. Mirasolia diversifolia fermented with 3% of L. bulgaricus for 5 d resulted in the lowest phytic acid content. Future research requires evaluating in vitro and in vivo dietary formulations for cattle using M. diversifolia fermented with L. bulgaricus.

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Section: Resultsmentioning

confidence: 99%

Pre-treatments of Mirasolia diversifolia using Lactobacillus bulgaricus at different dosages and fermentation times: Phytic acid concentration, enzyme activity, and fermentation characteristics

Pazla,

Putri,

Jamarun

et al. 2024

SA J. An. Sci.

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“…They studied the toxicity effects of PhyG (bacterial phytase variant) developed via fermentation using Trichoderma reesei and observed non-toxic when consumed on remarkably higher doses of consumption in broilers. Another study by Thorsen et al (2021) evaluated the safety of Phytase HM derived from Aspergillus oryzae . They studied the effects in vivo and observed no mutagenic or inflammatory effects.…”

Section: Introductionmentioning

confidence: 99%

Insight into phytase-producing microorganisms for phytate solubilization and soil sustainability

et al. 2023

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The increasing demand for food has increased dependence on chemical fertilizers that promote rapid growth and yield as well as produce toxicity and negatively affect nutritional value. Therefore, researchers are focusing on alternatives that are safe for consumption, non-toxic, cost-effective production process, and high yielding, and that require readily available substrates for mass production. The potential industrial applications of microbial enzymes have grown significantly and are still rising in the 21st century to fulfill the needs of a population that is expanding quickly and to deal with the depletion of natural resources. Due to the high demand for such enzymes, phytases have undergone extensive research to lower the amount of phytate in human food and animal feed. They constitute efficient enzymatic groups that can solubilize phytate and thus provide plants with an enriched environment. Phytases can be extracted from a variety of sources such as plants, animals, and microorganisms. Compared to plant and animal-based phytases, microbial phytases have been identified as competent, stable, and promising bioinoculants. Many reports suggest that microbial phytase can undergo mass production procedures with the use of readily available substrates. Phytases neither involve the use of any toxic chemicals during the extraction nor release any such chemicals; thus, they qualify as bioinoculants and support soil sustainability. In addition, phytase genes are now inserted into new plants/crops to enhance transgenic plants reducing the need for supplemental inorganic phosphates and phosphate accumulation in the environment. The current review covers the significance of phytase in the agriculture system, emphasizing its source, action mechanism, and vast applications.

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“…Phytase, a phosphatase enzyme, can hydrolyze phytic acid (Inositolhexakisphosphate) to inositol, inorganic phosphate, and potentially chelated minerals which are readily absorbed by monogastric animals e.g. poultry and fish (Ajith et al, 2018).The main objective of phytase supplementation in animal diet is to (i) use the inorganic P source and other important minerals already present in the plant-based feed (ii)improve the availability of Myo-inositol (iii) counter the anti-nutritional effect of phytate (iv) conserve non-renewable sources of phosphorus due to decreasing requirement of its addition in the animal feed (v) minimize the environmental excretion of phosphate (Mahmood et al, 2021;Thorsen et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Stimulatory effect of medium components on phytase production by Aspergillus niger and biotechnological application as a poultry feed additive

Mahmood

Shahid

Nadeem

et al. 2022

KJS publishes peer-review articles in Mathematics, Computer Sci

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The present study was conducted for the phytase production using solid-state fermentation (SSF) by Aspergillus niger. Optimization of various medium components was carried out for better production of phytase. Maximum enzyme activity (265 IU/g) was obtained, when the fungus was grown in the optimized culture medium containing glucose (1%), NH4NO3 (0.5%), FeSO4.7H2O (0.1%), KCl (0.1%), and MgSO4.7H2O (0.1%) at 35 °C after 5 days of incubation time with pH 6.Then phytase was supplemented as a poultry feed additive and given to broiler chickens during a feeding trial of 5 weeks. For this purpose, the birds of the control group (T0) were fed on a basal diet without external phytase, whereas, the birds of the experimental group (T3) were given basal feed + 3000 IU Phytase per Kg diet. The results exhibited that there was an improvement in the body weight gain (BWG) of chicks i.e. 1903 g-2090 g for the control group (T0) and experimental group (T3), respectively. The current study thus indicated the affectivity of phytase as a supplement in the broilers diet for better growth performance and recommended its use as an efficient additive in poultry feed formulation.

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Safety and efficacy profile of a phytase produced by fermentation and used as a feed additive

Cited by 12 publications

References 36 publications

Pre-treatments of <i>Mirasolia diversifolia</i> using <i>Lactobacillus bulgaricus</i> at different dosages and fermentation times: Phytic acid concentration, enzyme activity, and fermentation characteristics

Pre-treatments of <i>Mirasolia diversifolia</i> using <i>Lactobacillus bulgaricus</i> at different dosages and fermentation times: Phytic acid concentration, enzyme activity, and fermentation characteristics

Insight into phytase-producing microorganisms for phytate solubilization and soil sustainability

Stimulatory effect of medium components on phytase production by Aspergillus niger and biotechnological application as a poultry feed additive

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