Safety and efficacy of rice seed-based oral allergy vaccine for Japanese cedar pollinosis in Japanese monkeys

Sakai, Saburo; Takagi, Hidenori; Wakasa, Yuhya; Ozawa, Kyoko; Takaiwa, Fumio

doi:10.1016/j.molimm.2020.06.019

Cited by 7 publications

(5 citation statements)

References 43 publications

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“…The results open the window to the possibility of developing a new convenient route of oral allergen-specific immunotherapy that could be safely administered by the patient at home. A similar idea was demonstrated by Saito et al They used a transgenic rice seed-based oral allergy vaccine to treat Japanese cedar pollen allergic Japanese monkeys, and noted a suppression of allergen-specific cell proliferation after only two months of administration [35].…”

Section: Plos Onementioning

confidence: 60%

“…Gram-positive non-pathogenic lactic acid bacteria have long been widely used in the food industry. The protective or modulatory effects of recombinant L.lactis strains for several diseases have been verified in animal models and clinical trials [29][30][31][32][33][34][35]. In this study, we developed a recombinant L. lactis vaccine containing three of the most common indoor aeroallergens and investigated its effectiveness and safety for preventing respiratory allergy in mice.…”

Section: Introductionmentioning

confidence: 99%

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Effectiveness and safety of oral lactococci-based vaccine encoding triple common allergens to prevent airway allergy in mice

et al. 2021

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Allergic airway disease is the most common chronic airway inflammatory disorder in developed countries. House dust mite, cockroach, and mold are the leading allergens in most tropical and subtropical countries, including Taiwan. As allergen avoidance is difficult for patients allergic to these perennial indoor allergens, allergen-specific immunotherapy (ASIT) is the only available allergen-specific and disease-modifying treatment. However, for patients sensitized to multiple allergens, ASIT using each corresponding allergen is cumbersome. In the present study, we developed a recombinant L. lactis vaccine against the three most common indoor aeroallergens and investigated its effectiveness for preventing respiratory allergy and safety in mice. Three recombinant clones of Der p 2 (mite), Per a 2 (roach), and Cla c 14 (mold) were constructed individually in pNZ8149 vector and then electroporated into host strain L.lactis NZ3900. BALB/c mice were fed with the triple vaccine 5 times per week for 4 weeks prior to sensitization. The effectiveness and safety profile were then determined. Oral administration of the triple vaccine significantly alleviated allergen-induced airway hyper-responsiveness in the vaccinated mice. The allergen-specific IgG2a was upregulated. IL-4 and IL-13 mRNA expressions as well as inflammatory cell infiltration in the lungs decreased significantly in the vaccinated groups. No body weight loss or abnormal findings in the liver and kidneys were found in any of the groups of mice. This is the first report to describe a triple-aeroallergen vaccine using a food-grade lactococcal expression system. We developed a convenient oral delivery system and intend to extend this research to develop a vaccination that can be self-administered at home by patients.

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Section: Plos Onementioning

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Effectiveness and safety of oral lactococci-based vaccine encoding triple common allergens to prevent airway allergy in mice

et al. 2021

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“…The same principle has been used to produce recombinant allergy vaccines in rice seeds for allergen‐specific oral immunotherapy (Takaiwa et al ., 2015 ). A peptide vaccine‐containing seven linked human‐predominant T‐cell epitopes derived from Japanese cedar pollen allergens were also produced in rice (Saito et al ., 2020 ), and the efficacy and safety of the transgenic rice grains were confirmed after oral administration to JC pollinosis patients (Endo et al ., 2021 ).…”

Section: Oral Delivery Of Proteins To Confer Immunity or Tolerancementioning

confidence: 93%

Contributions of the international plant science community to the fight against infectious diseases in humans—part 2: Affordable drugs in edible plants for endemic and re‐emerging diseases

Baysal

Lobato-Gómez

et al. 2021

Plant Biotechnology Journal

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The fight against infectious diseases often focuses on epidemics and pandemics, which demand urgent resources and command attention from the health authorities and media. However, the vast majority of deaths caused by infectious diseases occur in endemic zones, particularly in developing countries, placing a disproportionate burden on underfunded health systems and often requiring international interventions. The provision of vaccines and other biologics is hampered not only by the high cost and limited scalability of traditional manufacturing platforms based on microbial and animal cells, but also by challenges caused by distribution and storage, particularly in regions without a complete cold chain. In this review article, we consider the

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“…Next, before clinical studies in humans, transgenic rice seeds were orally administered to naturally sensitized Japanese monkeys exhibiting similar clinical allergic symptoms. 53 Curative efficiency was suggested from results showing that suppression of allergen-specific PMBC proliferation was observed within just 2 months and allergen-specific IgE levels decreased in some monkeys. Furthermore, oral administration of transgenic rice to Japanese monkeys with JC pollinosis or healthy monkeys did not induce side effects.…”

Section: Modification Of Allergens (Design Of Allergy Vaccines)mentioning

confidence: 99%

Next-Generation Allergen-Specific Immunotherapy for Japanese Cedar Pollinosis Using Molecular Approaches

Takaiwa¹

2021

ITT

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Japanese cedar (JC) pollinosis is the most major IgE-mediated type I allergic disease in Japan. Allergen-specific immunotherapy is the only curative treatment for allergic diseases. Subcutaneous immunotherapy and sublingual immunotherapy have been introduced in Japan for JC pollinosis, but do not avoid some adverse side effects, because the natural allergens used as tolerogens cross-link with specific IgE types on mast cells and basophils. To make immunotherapy for JC pollinosis safer, more effective and convenient, rice-based oral allergy vaccines using hybrid peptides composed of multiple T-cell epitopes or recombinant deconstructed hypoallergenic derivatives derived from major allergens — Cry j 1 and Cry j 2 — have been developed and their efficacy and safety evaluated by oral administration of transgenic rice seeds. Furthermore, recombinant modified JC allergens conjugated with various immunomodulatory molecules and DNA-based vaccines have been created and their efficacy assessed.

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Safety and efficacy of rice seed-based oral allergy vaccine for Japanese cedar pollinosis in Japanese monkeys

Cited by 7 publications

References 43 publications

Effectiveness and safety of oral lactococci-based vaccine encoding triple common allergens to prevent airway allergy in mice

Effectiveness and safety of oral lactococci-based vaccine encoding triple common allergens to prevent airway allergy in mice

Contributions of the international plant science community to the fight against infectious diseases in humans—part 2: Affordable drugs in edible plants for endemic and re‐emerging diseases

Next-Generation Allergen-Specific Immunotherapy for Japanese Cedar Pollinosis Using Molecular Approaches

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