Safety and Efficacy of Ankaferd Blood Stopper in Dental Surgery.

Ercetin, Sevil; Haznedaroğlu, İbrahim C.; Kurt, Mevlüt; Önal, İbrahi̇m Koral; Aktaş, Alper; Kurt, Özlem Kar; Göker, Hakan; Özdemir, Oktay; Kirazlı, Şerafettin; Firat, Hüseyin

doi:10.4999/uhod.09032

Cited by 39 publications

(52 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Use of ABS as a hemostatic agent in external hemorrhages and in dental treatment in humans provided the first data showing that ABS was safe and effective in humans [9]. A phase I double-blind, randomized crossover, placebo-controlled clinical study with a 5-d washout period between the crossover periods that included healthy volunteers reported that ABS was safe [20].…”

Section: Nf-κbmentioning

confidence: 99%

“…The hemostatic effects of ABS have been observed in vitro and in vivo [12][13][14][15][16][17][18]. Use of ABS as a hemostatic agent in external hemorrhages and in dental treatment in humans provided the first data showing that ABS was safe and effective in humans [9]. A phase I randomized, double-blind crossover placebo-controlled clinical study performed with healthy volunteers reported that topical ABS usage was safe [20].…”

Section: Introductionmentioning

confidence: 99%

“…ABS is a hemostatic agent that can be used effectively in clinical practice to control external bleeding, dental and periodontal hemorrhaging, dermal bleed- Anahtar kelimeler: Ankaferd ® , endotel, transkripsiyon faktörleri, eritrosit ing, and/or superficial mucosal blood leakage [8][9][10][11]. The hemostatic effects of ABS have been observed in vitro and in vivo [12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The effects of Ankaferd Blood Stopper on transcription factors in HUVEC and erythrocyte protein profile

Yılmaz¹,

Güleç²,

Torun³

et al. 2011

Turk J Hematol

View full text Add to dashboard Cite

Objective: Ankaferd ® Blood Stopper (ABS) is an herbal extract that has historically been used as a hemostatic agent in traditional Turkish medicine. ABS is comprised of a standardized herbal mixture of T. vulgaris, G. glabra, V. vinifera, A. officinarum, and U. dioica. ABS's basic mechanism of action is the formation of an encapsulated protein web, which represents the focal point for vital erythrocyte masses. The hemostatic effects of ABS have been observed in vitro and in vivo. ABS was registered as a hemostatic agent for external hemorrhages and dental bleeding following phase I randomized, double-blind crossover placebo-controlled clinical research, and safety and efficacy reports. In terms of the potential use of ABS, transcription factors may be novel factors that play a role in the hemostatic and other pleiotropic effects of ABS. Materials and Methods: Hence, the present study aimed to investigate the effects of ABS on endothelium, and possible transcription factor changes in HUVEC (human umbilical vein endothelial cells) and the erythrocyte membrane profile. ABS (5 μL and 50 μL) was administered to HUVEC (in 75 cm 2 ; ~75% fullness) for 5 min and 15 min. Results: ABS caused significant increases in the level of activation of the following transcription factors; AP2, AR, CRE/ATF1, CREB, E2F1-5, E2F6, EGR, GATA, HNF-1, ISRE, Myc-Max, NF-1, NFkB, p53, PPAR, SMAD 2/3, SP1, TRE/AP1, and YY1. Following erythrocyte membrane isolation, protein complexes were undissolved, but denatured. The protein complex formed was resistant to heat and detergent. Trypsin and sonication were used in order to break this complex; the complex dissolved and erythrocyte membrane proteins were released in SDS-PAGE. Conclusion: ABS established a very fast and solid protein web, and increased the level of transcription factor activation. Therefore the cellular effects of ABS could be related to different intracellular biological pathways. (Turk J Hematol 2011; 28: 276-85)

show abstract

Section: Nf-κbmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The effects of Ankaferd Blood Stopper on transcription factors in HUVEC and erythrocyte protein profile

Yılmaz¹,

Güleç²,

Torun³

et al. 2011

Turk J Hematol

View full text Add to dashboard Cite

show abstract

“…[6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] It affects not only hemostasis but also wound healing with some antibacterial properties. [7][8][16][17][18][19][34][35][36][37][38][39] Clinical conditions of ABS use are increased in course of time as hemorrhagic problems persist despite available anti-hemorrhagic interventions.…”

Section: Discussionmentioning

confidence: 99%

Effects of Ankaferd Hemostat on Retinal Apoptosis in an Experimental Rabbit Retinal Model

Çıtırık¹,

Dilsiz²,

Haznedaroğlu³

et al. 2013

Turkiye Klinikleri J Med Sci

View full text Add to dashboard Cite

836nkaferd is an herbal extract, which has been used historically as a hemostatic agent in traditional Turkish medicine for centuries. The medicine comprised the standardized mixture of herbs Thymus vul- Effects of Ankaferd Hemostat on Retinal Apoptosis in an ExperimentalRabbit Retinal Model A AB BS ST TR RA AC CT T O Ob bj je ec ct ti iv ve e: : To assess ultrastructural alterations and cellular apoptotic effects of Ankaferd Blood Stopper ® (ABS) in an experimental rabbit retinal model. Retinal changes following the intravitreal injection of ABS were investigated on ten retina tissues of five rabbits. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : ABS was injected into the vitreous of right eye of five rabbits. The left eyes of rabbits received an intravitreal injection of balanced salt solution and were used as the control group. Oxidative damage, apoptosis, protein carbonylation and DNA fragmentation were studied. R Re es su ul lt ts s: : In the ABS injected eyes, the results of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) pattern of a few protein bands were up-regulated whereas some other bands showed down-regulation. The apoptosis was delayed, indicated by the morphological analyses and caspase activity in the ABS injected eyes. Furthermore, ABS-induced protein carbonyle formation and DNA fragmentation were also demonstrated in the rabbit retina. C Co on nc cl lu us si io on n: : ABS has de novo effects on apoptosis. ABS-induced down-regulations in the expressions of apoptotic molecules in the retinal microenvironment indicated that ABS might act as a topical biological response modifier. Intravitreal injection of ABS also has cytotoxic effects. Since ABS is currently being developed in basic and clinical grounds, those novel observations will guide future studies focusing on the pleiotropic effects of this unique hemostatic agent. K Ke ey y W Wo or rd ds s: : Ankaferd blood stoper; apoptosis; DNA fragmentation; intravitreal injections; protein carbonylation; retina Ö ÖZ ZE ET T A Am ma aç ç: : Deneysel tavşan retina modelinde Ankaferd Kan Durdurucu ® (ABS)'nun hücresel apopitotik etkisini ve neden olduğu ince yapısal değişiklikleri değerlendirmek. ABS'nin intravitreal enjeksiyonundan sonra beş tavşandaki on retina dokusu üzerinde meydana gelen değişiklikler incelendi. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Beş tavşanın sağ gözüne vitreus içine ABS enjekte edildi. Tavşanların sol gözleri kontrol grubu olarak kullanıldı ve vitreus içine dengeli tuz solüsyonu enjekte edildi. Oksidatif hasar, apopitoz, protein karbonilasyonu ve DNA fragmantasyonu incelendi. B Bu ul lg gu ul la ar r: : ABS enjekte edilen gözlerde, sodyum dodesil sülfat poliakrilamid jel elektroforezi (SDS-PAGE)'nde birkaç protein bandında artma ve bazı protein bantlarında azalma görüldü. ABS enjekte edilen gözlerde kaspaz aktivitesi ve morfolojik analizler ile tespit edilen apopitozda gecikme belirlendi. Ayrıca, tavşan retinalarında ABS'nin indüklediği protein karbonilasyonu oluşumu ve DNA fr...

show abstract

“…[33][34][35][36][37] Future experimental trials should focus on the in vivo ABS effects on renal disease expression patterns. ABS protein library and its tissue effects seem to be a starting point to begin designing those kinds of investigations in the kidney system.…”

Section: Discussionmentioning

confidence: 99%

Histopathological Alterations in the Kidney Tissue Following Topical Ankaferd Hemostat Application in a Rat Renal Injury Model

Karakoç¹,

Akar²,

Aksu³

et al. 2012

UHOD

View full text Add to dashboard Cite

Ankaferd Blood Stopper (ABS) is a novel topical hemostatic agent. ABS has been approved in Turkey for clinical hemorrhages, when the conventional control of bleeding by ligature and/or conventional hemostatic measures is ineffective. ABS has many cellular effects and could modulate numerous hemostatic proteins at the tissue and blood. ABS-induced formation of the protein network with vital erythroid aggregation covers the entire physiological hemostatic process. The aim of this study was to assess histopathological alterations due to topical ABS administration at the renal tissue level. Thirty-six Wistar rats weighing 70 to 80 gm were included into the study. The rats were divided into two groups as "the ABS-applied group" (ABS−G) and "the control group" (C−G). The animals in both groups were then again divided into the three subgroups of "postoperative (Postop.) 60th minutes", "Postop. 48th hours", and "Postop. 15th day". Therefore, there were six rats in each of the subgroups at the end of the analyses. The standard renal injury sites in the rats of ABS−G were applied 2 ml. of topical ABS, whereas 2 ml. of topical saline was applied to the renal injury sites of the rats in the C−G group. We detected significant erythrocyte aggregation and the accumulation of siderophages in the kidney tissue just after 60 minutes of ABS application persisting over 15 days. Our results indicated red blood cell accumulation and siderophages following the use of ABS are compatible with the suggested 'mechanism-of-action' of ABS that ABS-induced formation of the protein network with vital erythroid aggregation covers the entire physiological hemostatic process. Further experimental search is needed to find out the molecules inside the ABS protein library leading to the ABS-induced aggregation at the renal tissue level.

show abstract

Safety and Efficacy of Ankaferd Blood Stopper in Dental Surgery.

Cited by 39 publications

References 20 publications

The effects of Ankaferd Blood Stopper on transcription factors in HUVEC and erythrocyte protein profile

The effects of Ankaferd Blood Stopper on transcription factors in HUVEC and erythrocyte protein profile

Effects of Ankaferd Hemostat on Retinal Apoptosis in an Experimental Rabbit Retinal Model

Histopathological Alterations in the Kidney Tissue Following Topical Ankaferd Hemostat Application in a Rat Renal Injury Model

Contact Info

Product

Resources

About