Safety and Clinical Impact of a Single Red Light Irradiation on Breast Tumor‐Bearing Mice

Abstract

Low‐level light therapy has been used in health care as a therapeutic strategy for different diseases. However, its effects on cancer are controversial. This work evaluated the effects of three energies on breast cancer‐bearing mice after a single red light‐emitting diode (LED) irradiation. 4T1 cells were inoculated into the mammary fat pad of female BALB/c mice. When tumor volume reached 100 mm3, animals were irradiated by a LED irradiator (660 ± 11 nm) with energies of 1.2, 3.6, and 6.0 J. Control without irradiation and healthy animals were also evaluated. Mice were monitored regarding tumor volume and total blood count. After euthanasia, their organs were examined. We observed that a single irradiation does not increase tumor volume. All irradiated groups exhibited better clinical conditions than control, which presented a significant decrease in platelet and red blood cell levels compared with healthy mice. The energy of 3.6 J arrested neutrophil‐lymphocyte rate besides promoting longer survival and a lower number of metastatic nodules in the lungs. These findings suggest that a single red LED irradiation causes no impact on the course of the disease. Besides, the intermediary dose‐effect should be further investigated since it seems to promote better outcomes on breast cancer‐bearing mice.

Noninvasive Red Laser Intervention before Radiotherapy of Triple-negative Breast Cancer in a Murine Model

“…A trained and blinded veterinarian assigned scores for health status for each animal such as hypokinesia (0 = normal activity; 1 = reduced activity; 5 = inactive), piloerection (0 = no; 1 = yes), and hunching (0 = no; 1 = yes) ranging from 0 to 7. Higher scores indicate pain and distress, while lower scores denote better health status ( 23 , 25 ). The sum of the scores was computed for each experimental day.…”