Safeguarding the catalytic activity and stability of polyaniline chitosan silver nanocomposite bound beta-galactosidase against product inhibitors and structurally related compound

Khan, Maryam; Husain, Qayyum

doi:10.1080/21691401.2019.1593189

Cited by 11 publications

(3 citation statements)

References 42 publications

(47 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Differential scanning calorimetry (DSC) analysis 24 was carried out using DSC (Setaram, DSC131evo, France,). The samples were chitosan, vitamin C, mixture of chitosan and vitamin C (1:1), and the CSVC complex.…”

Section: Experimental Methodsmentioning

confidence: 99%

Study on Preparation of a Chitosan/Vitamin C Complex and Its Properties in Cosmetics

Liu

Ding

et al. 2020

Natural Product Communications

View full text Add to dashboard Cite

In order to expand the application of chitosan in cosmetics, it was modified with vitamin C to prepare a chitosan/vitamin C complex (CSVC complex), which was characterized using ultraviolet-visual, Fourier transform infrared, and differential scanning calorimetry techniques. Its hygroscopicity, moisture retention, antioxidant and antibacterial activities, and environmental stability were also determined. The results showed that a mass ratio of chitosan to vitamin C of 1:1 that reacted at 25℃ for 90 minutes in a medium of isopropanol–water, away from light, with the unreacted vitamin C being removed by dialysis gave a white powder (CSVC complex) after freeze-drying. The CSVC complex had good water solubility, a viscosity average molecular weight of 233.7 kDa, and a vitamin C content of 22.64%. Structure characterization confirmed that the CSVC complex was composed of the amine (C-2 position) of chitosan and the hydroxyl group (C-3 position) of vitamin C by electrostatic attraction. The complex had a significantly better scavenging effect on hydroxyl free radicals than chitosan, and the minimum inhibitory concentration against Escherichia coli was 5 mg/mL. Its moisturizing and hygroscopic properties were better than chitosan, and its stability was better than that of vitamin C. These results provide the basis for the CSVC complex as a multifunctional raw material for cosmetic use because of its antioxidant, moisturizing, antibacterial, and film-forming properties.

show abstract

Section: Experimental Methodsmentioning

confidence: 99%

Study on Preparation of a Chitosan/Vitamin C Complex and Its Properties in Cosmetics

Liu

Ding

et al. 2020

Natural Product Communications

View full text Add to dashboard Cite

show abstract

“…The intestinal microvilli secrete β-galactosidase (EC 3.2.1.23), commonly known as lactase in the jejunum (4,20,21). It is synthesized as a precursor peptide of 220 kDa that goes through post-translational modifications as it gets transported to the cell surface, where it is still in its inactive form.…”

Section: β-Galactosidasementioning

confidence: 99%

Intolerance to Milk Lactose, Diagnostic Tests and Dietary Management: A Recent Update

Shafi

Husain

2022

Avicenna J Med Biochem

Self Cite

View full text Add to dashboard Cite

Lactose intolerance is a common pathology that occurs due to the reduced activity of β-galactosidase leaving undigested lactose in the intestine. About 70% of the world population suffers from this condition. The gastro intestinal symptoms associated with this condition are diarrhoea, pain, nausea, bloating, flatulence, etc. It has been reported that these individuals are at a risk of developing several other pathologies like irritable bowel disease, osteoporosis, etc. Hence, proper diagnosis and treatment is essential for dealing with this condition. Various methods are used for providing an accurate diagnosis, such as hydrogen breath test (HBT), lactose intolerance test, genetic test, intestinal biopsy, etc. Depending on the type of intolerance, several methods are adopted for treating it, such as replacing enzyme, using exogenous enzymes, following lactose free diet, as well as consuming prebiotics and probiotics. Different methods are applied to synthesize lactose free dairy products to help lactose intolerant individuals suffering from important vitamins and minerals deprivation. Recently, plant-based milks are also used as a substitute for providing calcium and vitamins. The last few years have seen improvement in the quality and availability of lactose-free dairy products offering tempting foodstuffs to consumers. This narrative article aimed to review the existing science on lactose intolerance, along with its epidemiology, diagnosis, and clinical management.

show abstract

“…Aniline have been oxidized to polyaniline (PANi) using silver nitrate [24] or silver wire [14,25]. Subsequently, Ag+ ions are reduced to AgNPs [24,[26][27][28][29][30][31][32][33][34]. Doing so, both conductive PANi and conductive AgNps are produced.…”

Section: Introductionmentioning

confidence: 99%

A novel method for the preparation of conductive methyl cellulose-silver nanoparticles-polyaniline-nanocomposite

et al. 2020

View full text Add to dashboard Cite

A NOvel green method for the synthesis of conductive nanocomposite-using silver nitrate, methyl cellulose (MC), glycerol, and aniline-was adopted. Here, silver nitrate was used as a precursor, methyl cellulose as both reducing and capping agent, glycerol as a co-reducing and ductility agent and water as solvent. While silver nitrate oxidizes aniline to polyaniline, it is reduced to silver nanoparticles. Doing so, both conductive polyaniline and conductive silver nanoparticle were in-situ synthesized via the formation of methyl cellulosesilver-polyaniline nanocomposite. Reproducibility of the nanocomposite revealed that the synthesis process is consistent. The nanocomposite film was characterized by FTIR, SEM, eDAX, XRD, and conductivity. XRD pattern showed poly crystallinity. TeM of the colloidal solution showed different particle shapes of AgNPs with size from 4-36 nm and d-spacing of 0.22 nm. SAED exhibits ring pattern with bright spots displaying polycrystallinity. Nanocomposite film, coated glass, cotton and polyester fabrics showed promising conductivity.

show abstract

Safeguarding the catalytic activity and stability of polyaniline chitosan silver nanocomposite bound beta-galactosidase against product inhibitors and structurally related compound

Cited by 11 publications

References 42 publications

Study on Preparation of a Chitosan/Vitamin C Complex and Its Properties in Cosmetics

Study on Preparation of a Chitosan/Vitamin C Complex and Its Properties in Cosmetics

Intolerance to Milk Lactose, Diagnostic Tests and Dietary Management: A Recent Update

A novel method for the preparation of conductive methyl cellulose-silver nanoparticles-polyaniline-nanocomposite

Contact Info

Product

Resources

About