Safe therapeutics of murine melanoma model using a novel antineoplasic, the partially methylated mannogalactan from Pleurotus eryngii

Abstract

A heteropolysaccharide was isolated by cold aqueous extraction from edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps and its biological properties were evaluated. Structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses. A mannogalactan having a main chain of (1→6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl residues, both partially substituted at OH-2 by β-d-Manp (MG-Pe) single-unit was found. Biological effects of mannogalactan from P. eryngii (MG-Pe) were tested against murine melanoma cells. MG-Pe was non-cytotoxic, but reduced in vitro melanoma cells invasion. Also, 50mg/kg MG-Pe administration to melanoma-bearing C57BL/6 mice up to 10days decreased in 60% the tumor volume compared to control. Additionally, no changes were observed when biochemical profile, complete blood cells count (CBC), organs, and body weight were analyzed. Mg-Pe was shown to be a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice.

Anti-Thrombin, Anti-Adhesive, Anti-Migratory, and Anti-Proliferative Activities of Sulfated Galactans from the Tropical Green Seaweed, Udotea flabellum

“…However, such drugs might damage or kill normal and healthy cells. Therefore, recently, there is a search for compounds that do not alter the cellular viability, but rather cause cellular modifications that lead to antitumor effects, with minimum side effects [40,41]. Based on this, the results presented on this study reveal a promising agent against melanoma cells, which is a potential candidate for in vivo evaluation of its antitumor effect in future studies.…”

MG-Pe: A Novel Galectin-3 Ligand with Antimelanoma Properties and Adjuvant Effects to Dacarbazine

“…We previously demonstrated that the administration of partially methylated mannogalactan extracted from mushroom Pleurotus eryngii (MG- Pe ) (50 mg/kg) inhibited primary melanoma tumor growth ( versus control PBS) without inducing toxicity [ 15 ]. Here, in this study, we investigated MG- Pe treatment versus dacarbazine (DTIC—chemotherapy agent with known antitumor effect), alone or in combination (adjuvant effect).…”

“…Given that galectins bind glycans contain galactose moieties, and MG- Pe has galactose as a component [ 15 ], we sought to assess whether Gal-3/MG- Pe interaction could mediate the antitumor effects of this polysaccharide. We, therefore, evaluated MG- Pe direct binding to Gal-3 using physicochemical methods.…”

“…Our group has described non-toxic compounds with antitumor properties, a partially methylated mannogalactan (MG- Pe ), extracted from the edible mushroom Pleurotus eryngii . MG- Pe showed a significant antimelanoma activity modulating in vitro (B16-F10 murine cell) malignancy-related cell processes, such as cell invasion, and reducing solid tumor development in vivo without toxicity [ 15 ].…”

Mycochemicals in wild and cultivated mushrooms: nutrition and health

“…Recently, a mannogalactan with the main chain of (1 ? 6)-linked-a-D-galactopyranosyl and 3-Omethyl-a-D-galactopyranosyl residues, both partially substituted at OH-2 by b-D-Manp units was isolated from P. eryngii and tested against murine melanoma cells (Biscaia et al 2017). Zhang et al (2014) isolated three subfractions of intracellular zinc polysaccharides (IZPS) from P. cornucopiae.…”